Supplementation Effect of Early Pregnancy Factor‐Positive Serum into Bovine In Vitro Fertilization Culture Medium

PROBLEM: Early pregnancy factor (EPF) has been detected in pregnant bovine serum, and its activity appeared from 24 to 48 hr after insemination. However, in bovine in vitro fertilization (IVF), an EPF‐like substance(s) had been detected in the culture medium of fertilized ovum. Therefore, we think that EPF and EPF‐like substance(s) are very important materials for the development of the embryo. In this study, we examined the development of the embryo when fertilized bovine ova were cultured with IVF culture medium supplemented with EPF‐positive or ‐negative serum.

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