This study was conducted to evaluate the nutrients intake and the productive performance ofdairy cows and steers fed diets containing glycerol. The animals were distributed in a completely randomized design with factorial arrangement 4 glycerol levels (0, 6, 12 and 24%) x2sexes (steers and cows) and three replications. The consumption of dry matter (DM) crude protein (CP) and non-fiber carbohydrates (NFC) were not affected by inclusion of glycerol in the diets of cows and steers.However, there was a linear decrease in the consumption of neutral detergent fiber (NDF) (g/kg BW) for cows, but it did not affect the consumption of NDF and ethereal extract (EE) for steers when expressed in kg/day, but showed a quadratic response to NDF intake expressed as%BW and a linear decrease for the consumption of EE expressed in g/kgBW 0.75 and %BW. The cows showed a higher intake of DM, CP, NDF, NFC, expressed in kg/day, than the steers, when the diets included 6 and 12% glycerol and higher intakes of DM, NDF and NFC, expressed in g/kg/BW 0.75 , when diet included 6% glycerol. The daily weight gain was not affected by the inclusion of glycerol. We concluded that glycerol can be included up to 24% of total DM of diets ofdairy steers and cows without compromising animal performance. KEYWORDS: biodiesel byproducts; crude glycerin; nutritional value.