DNA unwinding produced by site-specific intrastrand cross-links of the antitumor drug cis-diamminedichloroplatinum(II).

The DNA unwinding produced by specific adducts of the antitumor drug cis-diamminedichloroplatinum(II) has been quantitatively determined. Synthetic DNA duplex oligonucleotides of varying lengths with two base pair cohesive ends were synthesized and characterized that contained site-specific intrastrand N7-purine/N7-purine cross-links. Included are cis-[Pt(NH3)2[d(GpG)]], cis-[Pt(NH3)2(d(ApG)]], and cis-[Pt(NH3)2[d(GpTpG)]] adducts, respectively referred to as cis-GG, cis-AG, and cis-GTG. Local DNA distortions at the site of platination were amplified by polymerization of these monomers and quantitatively evaluated by using polyacrylamide gel electrophoresis. The extent of DNA unwinding was determined by systematically varying the interplatinum distance, or phasing, in polymers containing the adducts. The multimer that migrates most slowly gives the optimal phasing for cooperative bending, from which the degree of unwinding can be obtained. We find that the cis-GG and cis-AG adducts both unwind DNA by 13 degrees, while the cis-GTG adduct unwinds DNA by 23 degrees. In addition, experiments are presented that support previous studies revealing that a hinge joint forms at the sites of platination in DNA molecules containing trans-GTG adducts. On the basis of an analysis of the present and other published studies of site-specifically modified DNA, we propose that local duplex unwinding is a major determinant in the recognition of DNA damage by the Escherichia coli (A)BC excinuclease. In addition, local duplex unwinding of 13 degrees and bending by 35 degrees are shown to correlate well with the recognition of platinated DNA by a previously identified damage recognition protein (DRP) in human cells.

[1]  L. Grossman,et al.  Dimerization of Escherichia coli UvrA and its binding to undamaged and ultraviolet light damaged DNA. , 1991, Biochemistry.

[2]  J. Hearst,et al.  Site-specific mutagenesis of conserved residues within Walker A and B sequences of Escherichia coli UvrA protein. , 1991, Biochemistry.

[3]  A. Sancar,et al.  Isolation and characterization of functional domains of UvrA. , 1991, Biochemistry.

[4]  D. Crothers,et al.  Helical repeat and chirality effects on DNA gel electrophoretic mobility. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[5]  W. Sundquist,et al.  Mechanistic studies of a novel class of trisubstituted platinum(II) antitumor agents. , 1991, Cancer research.

[6]  A. Rahmouni,et al.  Interstrand cross-links are preferentially formed at the d(GC) sites in the reaction between cis-diamminedichloroplatinum (II) and DNA. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[7]  C. Job,et al.  Transcription by eucaryotic and procaryotic RNA polymerases of DNA modified at a d(GG) or a d(AG) site by the antitumor drug cis-diamminedichloroplatinum(II). , 1991, Biochemistry.

[8]  S. Chaney,et al.  Role of carrier ligand in platinum resistance in L1210 cells. , 1990, Cancer research.

[9]  S. Lippard,et al.  Effect of the antitumor drug cis-diamminedichloroplatinum(II) and related platinum complexes on eukaryotic DNA replication. , 1990, Biochemistry.

[10]  A. Sancar,et al.  Structure and function of the (A)BC excinuclease of Escherichia coli. , 1990, Mutation research.

[11]  L. Grossman,et al.  The UvrABC endonuclease system of Escherichia coli--a view from Baltimore. , 1990, Mutation research.

[12]  M. Leng,et al.  Distortions induced in double-stranded oligonucleotides by the binding of cis- or trans-diammine-dichloroplatinum(II) to the d(GTG) sequence. , 1990, Nucleic acids research.

[13]  R. Fuchs,et al.  Single d(ApG)/cis-diamminedichloroplatinum(II) adduct-induced mutagenesis in Escherichia coli. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[14]  D. K. Treiber,et al.  Characterization of a DNA damage-recognition protein from mammalian cells that binds specifically to intrastrand d(GpG) and d(ApG) DNA adducts of the anticancer drug cisplatin. , 1990, Biochemistry.

[15]  A. Sancar,et al.  Molecular mechanisms of DNA repair inhibition by caffeine. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[16]  S. Lippard,et al.  Bending studies of DNA site-specifically modified by cisplatin, trans-diamminedichloroplatinum(II) and cis-[Pt(NH3)2(N3-cytosine)Cl]+. , 1990, Biophysical chemistry.

[17]  M. Leng.  DNA bending induced by covalently bound drugs. Gel electrophoresis and chemical probe studies. , 1990, Biophysical chemistry.

[18]  P. Hagerman,et al.  Application of the method of phage T4 DNA ligase-catalyzed ring-closure to the study of DNA structure. I. Computational analysis. , 1990, Journal of molecular biology.

[19]  S. Chaney,et al.  Effect of the diaminocyclohexane carrier ligand on platinum adduct formation, repair, and lethality. , 1990, Biochemistry.

[20]  S. Lippard,et al.  Isolation of cDNAs encoding a human protein that binds selectively to DNA modified by the anticancer drug cis-diamminedichloroplatinum(II) , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[21]  A. Sancar,et al.  大腸菌(A)BCエクシヌクレアーゼの構造と機能 | 文献情報 | J-GLOBAL 科学技術総合リンクセンター , 1990 .

[22]  R. Wood,et al.  Use of in vivo and in vitro assays for the characterization of mammalian excision repair and isolation of repair proteins. , 1990, Mutation research.

[23]  A. Sancar,et al.  A new mechanism for repairing oxidative damage to DNA: (A)BC excinuclease removes AP sites and thymine glycols from DNA. , 1989, Biochemistry.

[24]  D M Crothers,et al.  Artificial nucleosome positioning sequences. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[25]  A. Sancar,et al.  The (A)BC excinuclease of Escherichia coli has only the UvrB and UvrC subunits in the incision complex. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[26]  M. Leng,et al.  The DNA bending by acetylaminofluorene residues and by apurinic sites. , 1989, Journal of molecular biology.

[27]  D. Crothers,et al.  DNA bending by the bulge defect. , 1989, Biochemistry.

[28]  A. Eastman,et al.  DNA repair in cells sensitive and resistant to cis-diamminedichloroplatinum(II): host cell reactivation of damaged plasmid DNA. , 1989, Biochemistry.

[29]  M. Leng,et al.  Chemical probes of the conformation of DNA modified by cis-diamminedichloroplatinum(II). , 1989, Biochemistry.

[30]  D. Crothers,et al.  Phased psoralen cross-links do not bend the DNA double helix. , 1988, Biochemistry.

[31]  N. Cozzarelli,et al.  Helical repeat and linking number of surface-wrapped DNA. , 1988, Science.

[32]  A. Eastman,et al.  Enhanced DNA repair as a mechanism of resistance to cis-diamminedichloroplatinum(II). , 1988, Biochemistry.

[33]  S. Lippard,et al.  Chemical and biological studies of the major DNA adduct of cis-diamminedichloroplatinum(II), cis-[Pt(NH3)2(d(GpG]], built into a specific site in a viral genome. , 1988, Biochemistry.

[34]  D. Crothers,et al.  The major adduct of the antitumor drug cis-diamminedichloroplatinum(II) with DNA bends the duplex by approximately equal to 40 degrees toward the major groove. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[35]  J. Griffith,et al.  Thymine dimers bend DNA. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[36]  A. Sancar,et al.  DNA repair enzymes. , 1988, Annual review of biochemistry.

[37]  A. Klug,et al.  The bending of DNA in nucleosomes and its wider implications. , 1987, Philosophical transactions of the Royal Society of London. Series B, Biological sciences.

[38]  S. Lippard,et al.  Structural aspects of platinum anticancer drug interactions with DNA , 1987 .

[39]  A. V. van Oosterom,et al.  cis-Diamminedichloroplatinum(II)-induced DNA adducts in peripheral leukocytes from seven cancer patients: quantitative immunochemical detection of the adduct induction and removal after a single dose of cis-diamminedichloroplatinum(II). , 1987, Cancer research.

[40]  L. Grossman,et al.  Helicase properties of the Escherichia coli UvrAB protein complex. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[41]  L. Grossman,et al.  The effect of Escherichia coli Uvr protein binding on the topology of supercoiled DNA. , 1986, Nucleic acids research.

[42]  S. Lippard,et al.  Site-specifically platinated DNA, a new probe of the biological activity of platinum anticancer drugs , 1986 .

[43]  S. Holbrook,et al.  Action mechanism of ABC excision nuclease on a DNA substrate containing a psoralen crosslink at a defined position. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[44]  A. Eastman.  Reevaluation of interaction of cis-dichloro(ethylenediamine)platinum(II) with DNA. , 1986, Biochemistry.

[45]  D. Crothers,et al.  Topological distributions and the torsional rigidity of DNA. A Monte Carlo study of DNA circles. , 1986, Journal of molecular biology.

[46]  Hen-Ming Wu,et al.  DNA bending at adenine · thymine tracts , 1986, Nature.

[47]  S. Lippard,et al.  In vivo effects of cis- and trans-diamminedichloroplatinum(II) on SV40 chromosomes: differential repair, DNA-protein cross-linking, and inhibition of replication. , 1985, Biochemistry.

[48]  A. Sancar,et al.  Reactions of the UVRABC excision nuclease with DNA damaged by diamminedichloroplatinum(II). , 1985, Nucleic acids research.

[49]  M. Tang,et al.  Repair of psoralen and acetylaminofluorene DNA adducts by ABC excinuclease. , 1985, Journal of molecular biology.

[50]  S. Lippard,et al.  Sequence-dependent termination of in vitro DNA synthesis by cis- and trans-diamminedichloroplatinum (II). , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[51]  F. Collart,et al.  Unwinding of supercoiled DNA by cis- and trans-diamminedichloroplatinum(II): influence of the torsional strain on DNA unwinding. , 1985, Nucleic acids research.

[52]  P. Lohman,et al.  Adducts of the antitumor drug cis-diamminedichloroplatinum(II) with DNA: formation, identification, and quantitation. , 1985, Biochemistry.

[53]  Hen-Ming Wu,et al.  The locus of sequence-directed and protein-induced DNA bending , 1984, Nature.

[54]  M. Ciomei,et al.  Structural alterations of pathologically or physiologically modified DNA. , 1984, Nucleic acids research.

[55]  J. Wang,et al.  Torsional rigidity of DNA and length dependence of the free energy of DNA supercoiling. , 1984, Journal of molecular biology.

[56]  A. Sancar,et al.  A novel repair enzyme: UVRABC excision nuclease of Escherichia coli cuts a DNA strand on both sides of the damaged region , 1983, Cell.

[57]  O. Lumpkin.  Mobility of DNA in gel electrophoresis. , 1982, Biopolymers.

[58]  H. Frisch,et al.  Why does the electrophoretic mobility of DNA in gels vary with the length of the molecule? , 1982, Biopolymers.

[59]  W. Scovell,et al.  Cis- and trans-diamminedichloroplatinum(II) binding products different tertiary structural changes on SV40 DNA. , 1982, Biochemical and biophysical research communications.

[60]  A. M. Pedrini,et al.  Determination of pyrimidine dimer unwinding angle by measurement of DNA electrophoretic mobility. , 1982, Journal of molecular biology.

[61]  A. Klug,et al.  Helical periodicity of DNA determined by enzyme digestion , 1980, Nature.

[62]  S. Lippard,et al.  Binding of cis- and trans-dichlorodiammineplatinum(II) to the nucleosome core. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[63]  J. Barton,et al.  Binding of cis- and trans-dichlorodiammineplatinum(II) to DNA: evidence for unwinding and shortening of the double helix. , 1979, Science.

[64]  J. Wang,et al.  Helical repeat of DNA in solution. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[65]  J. Miller,et al.  Covalent intercalative binding to DNA in relation to the mutagenicity of hydrocarbon epoxides and N-acetoxy-2-acetylaminofluorene. , 1978, Cancer research.

[66]  W. Bauer,et al.  Structure and reactions of closed duplex DNA. , 1978, Annual review of biophysics and bioengineering.

[67]  F. B. Fuller.  The writhing number of a space curve. , 1971, Proceedings of the National Academy of Sciences of the United States of America.