LABEL-FREE QUANTITATIVE AND QUALITATIVE ANALYSIS OF ESCHERICHIA COLI GROWN ON DIFFERENT CARBON SOURCES

Many studies involving protein analysis of complex protein mixtures have been accomplished by combining the well-established separation capabilities of twodimensional polyacrylamide gel electrophoresis (2DPAGE) with mass spectrometry (MS)-based or tandem mass spectrometry (MS/MS)-based sequence identification of the gel-resolved proteins. Although 2DPAGE has been used successfully in quantitative proteomics, it is subject to mass range and pI limitations and it requires considerable effort to generate reproducible results. Gel spots may also contain more than one protein which will affect subsequent quantitative analysis.