Stereological estimation of average cell volume in monolayer culture by combined light and electron microscopy

In order to convert stereological ratio estimates into ‘absolute’ values (‘per cell’ values) the average cell volume must be estimated. The present paper describes a stereological method based on well‐known point counting procedures for the estimation of average cell volumes in monolayer cultures fixed in situ. This method involves estimation of the average attachment area per cell by light microscopy combined with estimation of the attachment membrane surface density by electron microscopy. There is no need for any assumption as to cellular or nuclear shape.

[1]  L M Cruz-Orive,et al.  Measuring error and sampling variation in stereology: comparison of the efficiency of various methods for planar image analysis , 1981, Journal of microscopy.

[2]  H. Gundersen,et al.  Notes on the estimation of the numerical density of arbitrary profiles: the edge effect , 1977 .

[3]  T. Mayhew,et al.  Ultrastructural morphometry of isolated cells: methods, models and applications. , 1980, Pathology, research and practice.

[4]  T. M. Mayhew,et al.  Caveat on the use of the Delesse principle of areal analysis for estimating component volume densities , 1974 .

[5]  E. Weibel,et al.  PRACTICAL STEREOLOGICAL METHODS FOR MORPHOMETRIC CYTOLOGY , 1966, The Journal of cell biology.

[6]  S Chien,et al.  Morphometry of human leukocytes. , 1980, Blood.

[7]  R. Østerby,et al.  Optimizing sampling efficiency of stereological studies in biology: or ‘Do more less well!‘ , 1981, Journal of microscopy.

[8]  K. H. Langer,et al.  Characterization of cells involved in the formation of granuloma , 1981, Virchows Archiv. B, Cell pathology including molecular pathology.

[9]  E. Beachey,et al.  Formation of multinucleated giant cells from human monocyte precursors. Mediation by a soluble protein from antigen-and mitogen-stimulated lymphocytes , 1982, The Journal of experimental medicine.

[10]  P. Katz,et al.  Human Blood Monocytes: Characterization of Negatively Selected Human Monocytes and Their Suspension Cell Culture Derivatives , 1981, Scandinavian journal of immunology.

[11]  L. Ko,et al.  Morphologic and morphometric analyses of butyrate-induced alterations of rat glioma cells in vitro. , 1980, Journal of the National Cancer Institute.

[12]  J M Papadimitriou,et al.  The measurement of cell volume and surface area by SEM photogrammetry , 1981, Journal of microscopy.

[13]  J. Lamvik,et al.  Structural and functional properties of blood monocytes cultured in vitro. , 2009, Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology.

[14]  G. Unsgaard,et al.  Activation of human monocytes by mediators from lymphocytes stimulated with Corynebacterium parvum. , 1979, Acta pathologica et microbiologica Scandinavica. Section C, Immunology.