The first gamma-H2AX biodosimetry intercomparison exercise of the developing European biodosimetry network RENEB.

In the event of a mass casualty radiation incident, the gamma-H2AX foci assay could be a useful tool to estimate radiation doses received by individuals. The rapid processing time of blood samples of just a few hours and the potential for batch processing, enabling high throughput, make the assay ideal for early triage categorisation to separate the 'worried well' from the low and critically exposed by quantifying radiation-induced foci in peripheral blood lymphocytes. Within the RENEB framework, 8 European laboratories have taken part in the first European gamma-H2AX biodosimetry exercise, which consisted of a telescoring comparison of 200 circulated foci images taken from 8 samples, and a comparison of 10 fresh blood lymphocyte samples that were shipped overnight to participating labs 4 or 24 h post-exposure. Despite large variations between laboratories in the dose-response relationship for foci induction, the obtained results indicate that the network should be able to use the gamma-H2AX assay for rapidly identifying the most severely exposed individuals within a cohort who could then be prioritised for accurate chromosome dosimetry.

[1]  E. Rogakou,et al.  Megabase Chromatin Domains Involved in DNA Double-Strand Breaks in Vivo , 1999, The Journal of cell biology.

[2]  Elizabeth A Ainsbury,et al.  Manual versus automated γ-H2AX foci analysis across five European laboratories: can this assay be used for rapid biodosimetry in a large scale radiation accident? , 2013, Mutation research.

[3]  Kai Rothkamm,et al.  Evidence for a lack of DNA double-strand break repair in human cells exposed to very low x-ray doses , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[4]  Y. Yao,et al.  Adapting the γ-H2AX Assay for Automated Processing in Human Lymphocytes. 1. Technological Aspects , 2011, Radiation research.

[5]  S. Baatout,et al.  Radiation-induced double strand breaks and subsequent apoptotic DNA fragmentation in human peripheral blood mononuclear cells. , 2012, International journal of molecular medicine.

[6]  Elizabeth A Ainsbury,et al.  DOSE ESTIMATION SOFTWARE FOR RADIATION BIODOSIMETRY , 2010, Health physics.

[7]  Kai Rothkamm,et al.  gamma-H2AX as protein biomarker for radiation exposure. , 2009, Annali dell'Istituto superiore di sanita.

[8]  William F. Blakely,et al.  The Use of Gamma-H2AX as a Biodosimeter for Total-Body Radiation Exposure in Non-Human Primates , 2010, PloS one.

[9]  Pascale Voisin,et al.  Quantification of γ-H2AX Foci in Human Lymphocytes: A Method for Biological Dosimetry after Ionizing Radiation Exposure , 2010, Radiation research.

[10]  A. Nakamura,et al.  Use of the γ-H2AX assay to monitor DNA damage and repair in translational cancer research. , 2012, Cancer letters.

[11]  F Trompier,et al.  Review of retrospective dosimetry techniques for external ionising radiation exposures. , 2011, Radiation protection dosimetry.

[12]  Kai Rothkamm,et al.  Candidate protein biomarkers as rapid indicators of radiation exposure , 2011 .

[13]  Kai Rothkamm,et al.  Inter-individual and inter-cell type variation in residual DNA damage after in vivo irradiation of human skin. , 2011, Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology.

[14]  Vicky Goh,et al.  Leukocyte DNA damage after multi-detector row CT: a quantitative biomarker of low-level radiation exposure. , 2007, Radiology.

[15]  Ulrike Kulka,et al.  Laboratory Intercomparison on the γ-H2AX Foci Assay , 2013, Radiation research.

[16]  Kai Rothkamm,et al.  The shape of the radiation dose response for DNA double-strand break induction and repair , 2013, Genome Integrity.

[17]  Volodymyr A Vinnikov,et al.  A comparison of six statistical distributions for analysis of chromosome aberration data for radiation biodosimetry. , 2013, Radiation protection dosimetry.

[18]  F Trompier,et al.  Realising the European Network of Biodosimetry (RENEB). , 2012, Radiation protection dosimetry.

[19]  Kai Rothkamm,et al.  Gamma-H2AX-Based Dose Estimation for Whole and Partial Body Radiation Exposure , 2011, PloS one.

[20]  H. Thierens,et al.  Dose-length product of scanners correlates with DNA damage in patients undergoing contrast CT. , 2012, European journal of radiology.

[21]  M. Uder,et al.  Induction and repair of DNA double-strand breaks in blood lymphocytes of patients undergoing 18F-FDG PET/CT examinations , 2012, European Journal of Nuclear Medicine and Molecular Imaging.