Antimicrobial and L-asparaginase activities of endophytic fungi isolated from Datura innoxia and Hyoscyamus muticus medicinal plants

Thirty-six species and two varieties belonging to 16 genera of fungal endophytes were isolated from the leaves of Datura innoxia and Hyoscyamus muticus plants. The most prevailing fungi were: Aspergillus fumigatus , A. niger , A. terreus var. africanus , Cladosporium cucumerinum, C . oxysporum , Penicil lium aurantiogriseum and P. chrysogenum . Endophytic fungi from D . innoxia and H . muticus plants were tested for antibacterial and antifungal activities from which 68.98 and  78.26% respectively showed antibacterial against at least one of the tested microbe, but didn’t had effect on tested fungal isolates. Aspergillus niger SVUAn1 was in the top in producing L-asparaginase among tested isolates obtained from two plants. Maximum production of this enzyme obtained after 4 days of incubation with culture medium containing glucose as a carbon source. This study indicated that the endophytic fungi from D . innoxia and H . muticus plant another potential source of bioactive antimicrobial and anticancer agents.

[1]  Y. Gherbawy,et al.  Endophytic fungi associated with high-altitude Juniperus trees and their antimicrobial activities , 2016 .

[2]  Qinggui Wang,et al.  Antibacterial and Antifungal Compounds from Marine Fungi , 2015, Marine drugs.

[3]  D. C. Raju,et al.  Isolation, characterization and antibacterial activity of endophytic fungi from Calophyllum inophyllum L , 2015 .

[4]  Amrutha. V. Audipudi,et al.  Screening for antimicrobial activities of endophytic fungi isolated from ripened fruit of Capsicum Frutescence L. , 2015 .

[5]  F. Pha,et al.  SCREENING OF ENDOPHYTIC FUNGI ISOLATED FROM CELASTRUS PANICULATUS FOR ANTIMICROBIAL POTENTIAL , 2015 .

[6]  I. Kalyanasundaram,et al.  PRODUCTION, PURIFICATION AND CHARACTERISATION OF EXTRACELLULAR L-ASPARAGINASE FROM SALT MARSH FUNGAL ENDOPHYTES , 2015 .

[7]  K. Raju.  OPTIMIZATION OF PROCESS PARAMETERS FOR L- ASPARAGINASE PRODUCTION BY Aspergillus terreus MTCC 1782 UNDER SOLID STATE FERMENTATION USING MIXED SUBSTRATE , 2015 .

[8]  S. Mirzaei,et al.  Asparaginase and amylase activity of thyme endophytic fungi , 2014 .

[9]  S. More,et al.  Isolation and production of clinical and food grade L- Asparaginase enzyme from fungi , 2014 .

[10]  Xinru Liu,et al.  Three new sterigmatocystin analogues from marine-derived fungus Aspergillus versicolor MF359 , 2014, Applied Microbiology and Biotechnology.

[11]  C. Srinivas,et al.  Antimicrobial activity and phytochemical analysis of crude extractsof endophytic fungi isolated from Plumeria acuminata L. and Plumeria obtusifolia L. , 2014 .

[12]  S. Sandhu,et al.  Isolation and detection of anti-bacterial activity of endophytic fungi from Bombex cebia and Argemone mexicana , 2014 .

[13]  S. Yagi,et al.  Endophytic Fungal Communities Associated with Ethno-medicinal Plants from Sudan and their Antimicrobial and Antioxidant Prospective , 2014 .

[14]  K. Selvi.  ISOLATION AND SCREENING OF ENDOPHYTIC FUNGI FROM MEDICINAL PLANTS OF VIRUDHUNAGAR DISTRICT FOR ANTIMICROBIAL ACTIVITY , 2014 .

[15]  S. Sarkar,et al.  Production of L-Asparaginase By Fusarium Oxysporum Using Submerged Fermentation , 2014 .

[16]  Devi Nn,et al.  Bioactive metabolites from an endophytic fungus Penicillium sp. isolated from Centella asiatica , 2014 .

[17]  Kumala Shirly,et al.  ISOLATION IPG3 - 1 AND IPG3 - 3 , ENDOPHYTIC FUNGI FROM DELIMA ( PU NICA GRANATUM LINN .) TWIGS AND IN VITRO ASSESSMENT OF THEIR ANTI MICROBIAL ACTIVITY , 2013 .

[18]  S. Singh,et al.  Endophytic fungi from Jatropha curcus: a preliminary study. , 2013 .

[19]  I. Ahmed,et al.  Production of L-Asparaginase from Aspergillus Niger using Agro Wastes By-Products in Submerged Fermentation Process , 2013 .

[20]  S. More,et al.  Isolation, Purification and Characterization of Fungal Extracellular L-Asparaginase from Mucor hiemalis , 2013 .

[21]  M. Shaaban,et al.  Bioactive secondary metabolites from endophytic Aspergillus fumigatus: structural elucidation and bioactivity studies , 2013 .

[22]  Randa ABDOUa.  BIOACTIVE METABOLITES FROM THE ENDOPHYTE BOTRYOSPHERIA OBTUSE OF THE MEDICINAL PLANT BIDENS PILOSA RANDA , 2013 .

[23]  Xiao‐Ming Li,et al.  Anthraquinone Derivatives Produced by Marine-Derived Fungus Aspergillus versicolor EN-7 , 2012, Bioscience, biotechnology, and biochemistry.

[24]  Radhika Tippani,et al.  Nutritional factors effecting the production of L-asparaginase by the Fusarium sp. , 2012 .

[25]  L. Sundaram,et al.  ISOLATION AND SCREENING OF L-ASPARAGINASE PRODUCING FUNGI FROM SOIL SAMPLES Research Article , 2012 .

[26]  Kamal Uddin Zaidi,et al.  L-Asparaginase: A Promising Enzyme for Treatment of Acute Lymphoblastic Leukiemia , 2012 .

[27]  M. V. Toledo,et al.  Science against microbial pathogens : communicating current research and technological advances , 2012 .

[28]  N. Gusmão.  Endophytic fungi from brazilian mangrove plant Laguncularia racemosa (L.) Gaertn. (Combretaceae): their antimicrobial potential , 2011 .

[29]  T. S. Murali,et al.  L-asparaginase from marine derived fungal endophytes of seaweeds , 2011 .

[30]  Mi Ae Kim,et al.  Bioactive metabolites from the sponge-derived fungus Aspergillus versicolor , 2010, Archives of pharmacal research.

[31]  S. Ito,et al.  ANTIFUNGAL ACTIVITY OF ENDOPHYTIC FUNGI ISOLATED FROM EGYPTIAN HENBANE (HYOSCYAMUS MUTICUS L.) , 2010 .

[32]  Shakeel A. Khan,et al.  Communities of endophytic fungi in medicinal plant Withania somnifera. , 2010 .

[33]  K. C. Biswal,et al.  METHODS: Optimization of culture medium for production of recombinant dengue protein in Escherichia coli , 2009 .

[34]  Mohammad Hossein Morowvat,et al.  An Optimized Medium for Screening of L-Asparaginase production by Escherichia coli , 2008 .

[35]  Y. L. Krishnamurthy,et al.  Fungal communities in herbaceous medicinal plants from the malnad region, southern India. , 2008, Microbes and environments.

[36]  R. Tan,et al.  Biology and chemistry of endophytes. , 2006, Natural product reports.

[37]  D. Goodsell.  The Molecular Perspective: l‐Asparaginase , 2005, Stem cells.

[38]  K. Sridhar,et al.  Antimicrobial and Enzyme Activity of Mangrove Endophytic Fungi of Southwest Coast of India , 2005 .

[39]  S. Talhouk,et al.  Screening of selected indigenous plants of Lebanon for antimicrobial activity. , 2004, Journal of ethnopharmacology.

[40]  Maria Francisca Simas Teixeira,et al.  Endophytic fungi from Melia azedarach , 2003 .

[41]  Siddalingeshwara K.G.  Production and Characterization of L- Asparaginase-A Tumour inhibitor , 2003 .

[42]  H. Chun,et al.  Design and synthesis of highly potent fumagillin analogues from homology modeling for a human MetAP-2. , 2000, Bioorganic & medicinal chemistry letters.

[43]  A. Rossman,et al.  Protocols for an All Taxa Biodiversity Inventory of Fungi in a Costa Rican Conservation Area , 1997 .

[44]  R. K. Saxena,et al.  A rapid plate assay for screening l‐asparaginase producing micro‐organisms , 1997, Letters in applied microbiology.

[45]  G. Bills.  Isolation and analysis of endophytic fungal communities from woody plants. , 1996 .

[46]  K. Domsch,et al.  Compendium of Soil Fungi , 1995 .

[47]  J. Helenius,et al.  Toxicity of plant extracts to three storage beetles (Coleoptera) , 1992 .

[48]  T. Kinoshita,et al.  Synerazol, a new antifungal antibiotic. , 1991, The Journal of antibiotics.

[49]  B. Ganguli,et al.  Fumifungin, a new antifungal antibiotic from Aspergillus fumigatus Fresenius 1863. , 1987, The Journal of antibiotics.

[50]  R. K. Saxena,et al.  L-asparaginase and glutaminase activities in the culture filtrates of Aspergillus nidulans , 1981 .

[51]  E. Freireich,et al.  L‐asparaginase for the treatment of cancer , 1973, CA: a cancer journal for clinicians.

[52]  S. Igarasi,et al.  Asparaginase and glutaminase activities of micro-organisms. , 1973, Journal of general microbiology.

[53]  V. E. Zurita,et al.  [Production of l-asparaginase by Aspergillus terreus]. , 1971, Annali dell'Istituto superiore di sanita.

[54]  A. Bauer,et al.  Antibiotic susceptibility testing by a standardized single disk method. , 1966, American journal of clinical pathology.

[55]  J. Stevenson,et al.  A monograph of the Chaetomiaceae. , 1963 .

[56]  G. Martin.  The Genus Aspergillus. , 1946, Science.

[57]  K. Soytong.  The fungal endophyte dilemma , 2022 .