Hyper Rayleigh scattering of protein-mediated gold nanoparticles aggregates

The second harmonic generation response from protein-mediated gold nanoparticles assemblies in solution has been studied by the technique of hyper Rayleigh scattering (HRS). It is found that the HRS intensity from biotinylated bovine serum albumin coated gold nanoparticles is enhanced when StreptAvidin is added into the solution. This increase in intensity is attributed to the aggregation of the gold nanoparticles through the binding of biotin and StreptAvidin. Comparison with photo-absorption spectroscopy indicates that the technique of HRS is a potential tool in detecting small levels of particle aggregation in liquid samples.

[1]  Persoons,et al.  Hyper-Rayleigh scattering in solution. , 1991, Physical review letters.

[2]  Z. Kam,et al.  Absorption and Scattering of Light by Small Particles , 1998 .

[3]  Eric Hendrickx,et al.  Second-order nonlinear optics in isotropic liquids: Hyper-Rayleigh scattering in solution , 1995 .

[4]  D. D. Yue,et al.  Theory of Electric Polarization , 1974 .

[5]  Ethan A Merritt,et al.  Cooperative hydrogen bond interactions in the streptavidin–biotin system , 2006, Protein science : a publication of the Protein Society.

[6]  N. Thanh,et al.  Laser-based double beam absorption detection for aggregation immunoassays using gold nanoparticles , 2002, Analytical and bioanalytical chemistry.

[7]  A. Schuurs,et al.  Sol particle agglutination immunoassay for human chorionic gonadotrophin , 1980 .

[8]  Christian Jonin,et al.  Electric dipole origin of the second harmonic generation of small metallic particles , 2005 .

[9]  Nikolai G Khlebtsov,et al.  A protein assay based on colloidal gold conjugates with trypsin. , 2005, Analytical biochemistry.

[10]  Leigh B. Bangs,et al.  New developments in particle-based immunoassays: Introduction , 1996 .

[11]  E. Coronado,et al.  The Optical Properties of Metal Nanoparticles: The Influence of Size, Shape, and Dielectric Environment , 2003 .

[12]  Zeev Rosenzweig,et al.  Development of an aggregation-based immunoassay for anti-protein A using gold nanoparticles. , 2002, Analytical chemistry.

[13]  C. Plotz,et al.  The latex fixation test. I. Application to the serologic diagnosis of rheumatoid arthritis. , 1956, The American journal of medicine.

[14]  J. Hupp,et al.  Enormous Hyper-Rayleigh Scattering from Nanocrystalline Gold Particle Suspensions , 1998 .

[15]  R. W. Christy,et al.  Optical Constants of the Noble Metals , 1972 .

[16]  H. Girault,et al.  Enhancement of the Second Harmonic Response by Adsorbates on Gold Colloids: The Effect of Aggregation , 1999 .

[17]  S. Dähne,et al.  Anomalous fluorescence behaviour of organic dyes , 1974 .

[18]  Xin Wang,et al.  Hyper-Rayleigh scattering of protein-modified gold nanoparticles. , 2003, Analytical biochemistry.

[19]  Pierre-François Brevet,et al.  Wavelength dependence of the hyper Rayleigh scattering response from gold nanoparticles. , 2004, The Journal of chemical physics.

[20]  Isabelle Russier-Antoine,et al.  Multipolar Contributions of the Second Harmonic Generation from Silver and Gold Nanoparticles , 2007 .