Distributed fiber-optic sensors employ the conventional fiber as the sensing medium and allow all locations along the optical fiber to be measured simultaneously. Thus the sensing scheme provides an efficient way of simultaneously achieving multi-points sensing with a convenient and low-cost configuration. One of the most popular technologies that enables distributed sensing is based on Brillouin scattering. Characteristics of Brillouin scattering are determined mostly by the material properties of the optical fiber and the Brillouin Frequency Shift (BFS) has linear dependence on both the temperature and strain of the fiber under test (FUT). Based on this principle, intensive research efforts have been made in distributed Brillouin fiber sensors during the past two decades. Among them, Brillouin Optical Time-Domain Analysis (BOTDA) has attracted intensive research interest due to its promising properties for temperature and strain monitoring. In BOTDA the local Brillouin Gain Spectrum (BGS) is reconstructed by scanning the frequency offset of a continuous wave and an optical pulse around BFS when they are counter-propagating inside FUT, and the local BFS and hence the temperature and/ or strain information are retrieved accordingly. Usually curve fitting techniques are employed in determining the BFSs of the measured BGSs along the FUT, in which the measured BGS is fitted using an ideal curve and the BFS is found to be the frequency with the peak gain on the fitted curve. Fitting techniques using different lineshape for ideal curves, e.g. Lorentzian curve fitting (LCF), Gaussian curve fitting (GCF), pseudo-Voigt curve fitting (pVCF), and quadratic curve fitting (QCF) have been used to find the BFS distribution along FUT. However, the curve fitting techniques require careful initialization of model parameters and as many data points as possible collected on the measured BGS to ensure the fitting accuracy, otherwise the accuracy in BFS determination will degrade significantly. In addition, long processing time is needed for the iteration of fitting algorithms to find the BFS, especially when the sensing distance is long with a large number of BGSs collected and processed. Recently we have utilized the nonlinear mapping capability of Artificial Neural Network (ANN) in a BOTDA system and has successfully extracted temperature distribution from the measured BGSs along the FUT. Before temperature extraction ANN is trained to acquire the knowledge about the BGS patterns under different temperatures, thus it allows better accuracy even if the data points on the collected BGS become fewer when large frequency scanning step is adopted during the acquisition of BGSs. Moreover, the processing speed of ANN for temperature extraction is much faster than that of curving fitting techniques. In this presentation, we will review our work on using ANN for temperature extraction in a BOTDA system. Different idea spectrum profiles are used for training ANN, and the corresponding performances are compared with those using conventional curve fitting techniques. Some potential applications using ANN in BOTDA are also discussed. We believe that ANN can be an attractive tool for direct temperature or strain extraction in BOTDA system at high accuracy and fast speed.