A highly sensitive impedimetric aptasensor for the selective detection of acetamiprid and atrazine based on microwires formed by platinum nanoparticles.

A novel impedimetric biosensor was developed for the detection of the two extensively used pesticides, acetamiprid and atrazine. By employing the sputtering and e-beam lithography techniques, platinum nanoparticles (Pt NPs) were deposited in a bridge-like arrangement, in between interdigitated electrodes (IDEs). The resulting Pt NP microwires were chemically functionalized to allow the covalent immobilization of aptamers against the two target analytes onto the sensor surfaces. The biosensing platform facilitated charge transfer through the microwire-bridged IDEs, while upon analyte binding to the immobilized aptamers electron transfer was hindered, resulting in an increase of the electrochemical cell's impedance. The combination of Pt NPs microwires and aptamers allowed the sensitive and highly selective detection of acetamiprid with a linear range of response in the range of 10pM to 100nM with a limit of detection (LoD) at 1pM, and of atrazine with a linear range of responses from 100pM to 1μM and a LoD at 10pM respectively. Its performance was tested against a number of other commonly used pesticides as well as in real water samples.

[1]  P. Tchounwou,et al.  Atrazine potentiation of arsenic trioxide‐induced cytotoxicity and gene expression in human liver carcinoma cells (HepG2) , 2001, Molecular and Cellular Biochemistry.

[2]  Kun Wang,et al.  Silver nanoparticles anchored on nitrogen-doped graphene as a novel electrochemical biosensing platform with enhanced sensitivity for aptamer-based pesticide assay. , 2015, The Analyst.

[3]  Hanwen Sun,et al.  Rapid selective accelerated solvent extraction and simultaneous determination of herbicide atrazine and its metabolites in fruit by ultra high performance liquid chromatography. , 2016, Journal of Separation Science.

[4]  Meihua Yang,et al.  A gold-based nanobeacon probe for fluorescence sensing of organophosphorus pesticides. , 2015, Analytica chimica acta.

[5]  J. Sudji,et al.  Cryptorchidism and pesticides: Is there a connection? , 2017, Journal of pediatric surgery.

[6]  Mohammad Ramezani,et al.  A novel electrochemical aptasensor based on arch-shape structure of aptamer-complimentary strand conjugate and exonuclease I for sensitive detection of streptomycin. , 2016, Biosensors & bioelectronics.

[7]  Gajendra S Shekhawat,et al.  Immunoanalytical techniques for analyzing pesticides in the environment , 2009 .

[8]  Tianyan You,et al.  Resonance energy transfer from CdTe quantum dots to gold nanorods using MWCNTs/rGO nanoribbons as efficient signal amplifier for fabricating visible-light-driven “on-off-on” photoelectrochemical acetamiprid aptasensor , 2016 .

[9]  Jiang He,et al.  Isolation and identification of the DNA aptamer target to acetamiprid. , 2011, Journal of agricultural and food chemistry.

[10]  Yanbin Li,et al.  An electrochemical aptasensor based on gold nanoparticles dotted graphene modified glassy carbon electrode for label-free detection of bisphenol A in milk samples. , 2014, Food chemistry.

[11]  R. Blizard,et al.  Autism genes are selectively targeted by environmental pollutants including pesticides, heavy metals, bisphenol A, phthalates and many others in food, cosmetics or household products , 2016, Neurochemistry International.

[12]  R. Mesnage,et al.  Major Pesticides Are More Toxic to Human Cells Than Their Declared Active Principles , 2014, BioMed research international.

[13]  M. Ishizuka,et al.  Relationship between Urinary N-Desmethyl-Acetamiprid and Typical Symptoms including Neurological Findings: A Prevalence Case-Control Study , 2015, PloS one.

[14]  Ki-Hyun Kim,et al.  Immunosensing of Atrazine with Antibody-Functionalized Cu-MOF Conducting Thin Films. , 2015, ACS applied materials & interfaces.

[15]  B. Janegitz,et al.  Determination of Atrazine in Natural Water Samples by Differential Pulse Adsorptive Stripping Voltammetry Using a Bismuth Film Electrode , 2012 .

[16]  Mark D. Miller,et al.  Childhood Leukemia: A Preventable Disease , 2016, Pediatrics.

[17]  Vikas Gupta,et al.  Potential pathways of pesticide action on erectile function – A contributory factor in male infertility , 2015 .

[18]  Marianneza Chatzipetrou,et al.  Surface functionalization studies and direct laser printing of oligonucleotides toward the fabrication of a micromembrane DNA capacitive biosensor , 2012 .

[19]  Guohua Zhao,et al.  Aptamer-based colorimetric sensing of acetamiprid in soil samples: sensitivity, selectivity and mechanism. , 2013, Journal of hazardous materials.

[20]  R. Cooper,et al.  Atrazine and reproductive function: mode and mechanism of action studies. , 2007, Birth defects research. Part B, Developmental and reproductive toxicology.

[21]  Robert J. Messinger,et al.  Making it stick: convection, reaction and diffusion in surface-based biosensors , 2008, Nature Biotechnology.

[22]  P. Sanchez.  DNA Aptamer Development for Detection of Atrazine and Protective Antigen Toxin Using Fluorescence Polarization , 2012 .

[23]  Jing Li,et al.  A sensitive and label-free photoelectrochemical aptasensor using Co-doped ZnO diluted magnetic semiconductor nanoparticles. , 2016, Biosensors & bioelectronics.

[24]  Baoxin Li,et al.  A simple and rapid chemiluminescence aptasensor for acetamiprid in contaminated samples: Sensitivity, selectivity and mechanism. , 2016, Biosensors & bioelectronics.

[25]  Kun Wang,et al.  Label-free impedimetric aptasensor for detection of femtomole level acetamiprid using gold nanoparticles decorated multiwalled carbon nanotube-reduced graphene oxide nanoribbon composites. , 2015, Biosensors & bioelectronics.

[26]  S. M. Taghdisi,et al.  Electrochemical aptamer based assay for the neonicotinoid insecticide acetamiprid based on the use of an unmodified gold electrode , 2017, Microchimica Acta.

[27]  Alessandra Bonanni,et al.  DNA hybridization detection by electrochemical impedance spectroscopy using interdigitated gold nanoelectrodes , 2010 .

[28]  C. Carter.  The barrier, airway particle clearance, placental and detoxification functions of autism susceptibility genes , 2016, Neurochemistry International.

[29]  Y. Gan,et al.  A new electrochemical platform for ultrasensitive detection of atrazine based on modified self-ordered Nb2O5 nanotube arrays , 2017 .

[30]  Guohua Zhao,et al.  A highly selective electrochemical impedance spectroscopy-based aptasensor for sensitive detection of acetamiprid. , 2013, Biosensors & bioelectronics.

[31]  David S. Wishart,et al.  Simulations of Interdigitated Electrode Interactions with Gold Nanoparticles for Impedance-Based Biosensing Applications , 2015, Sensors.

[32]  Akhtar Hayat,et al.  Aptamer based electrochemical sensors for emerging environmental pollutants , 2014, Front. Chem..

[33]  Kun Wang,et al.  A facile label-free colorimetric aptasensor for acetamiprid based on the peroxidase-like activity of hemin-functionalized reduced graphene oxide. , 2015, Biosensors & bioelectronics.

[34]  Richa Sharma,et al.  Recent advances in nanoparticle based aptasensors for food contaminants. , 2015, Biosensors & bioelectronics.

[35]  Susana Cardoso,et al.  Label-free disposable immunosensor for detection of atrazine. , 2016, Talanta.

[36]  Marianneza Chatzipetrou,et al.  Label-free DNA biosensor based on resistance change of platinum nanoparticles assemblies. , 2016, Biosensors & bioelectronics.

[37]  Anthony J. Killard,et al.  Atrazine analysis using an amperometric immunosensor based on single-chain antibody fragments and regeneration-free multi-calibrant measurement , 2003 .

[38]  M. Marco,et al.  Electrochemical biosensing of pesticide residues based on affinity biocomposite platforms. , 2007, Biosensors & bioelectronics.

[39]  E. Prossnitz,et al.  G-Protein–Coupled Receptor 30 and Estrogen Receptor-α Are Involved in the Proliferative Effects Induced by Atrazine in Ovarian Cancer Cells , 2008, Environmental health perspectives.

[40]  Jennifer Bethsass,et al.  European Union Bans Atrazine, While the United States Negotiates Continued Use , 2006, International journal of occupational and environmental health.

[41]  Marit Nilsen-Hamilton,et al.  Aptamers in analytics. , 2016, The Analyst.

[42]  Letha J. Sooter,et al.  In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element against Atrazine , 2014, International journal of molecular sciences.