The role of intermolecular interactions: studies on model systems for bacterial biofilms.

The mechanical stability of biofilms and other microbial aggregates is of great importance for both the maintenance of biofilm processes and the removal of undesired biofilms. The binding forces are weak interactions such as London dispersion forces, electrostatic interactions and hydrogen bonds. In a first attempt to rank their contribution, the viscosity of solutions of extracellular polymeric substances (EPS) from a mucoid strain of Pseudomonas aeruginosa is measured. In order to distinguish the binding forces, substances are chosen which individually address the different types of bonds. Polyacrylic acid is identified as a suitable model system for EPS when molecular interactions are studied. Electrostatic interactions and hydrogen bonds are found to be the dominating forces among macromolecules within the biofilm.

[1]  J. Bryers,et al.  Effects of carbon and oxygen limitations and calcium concentrations on biofilm removal processes , 1991, Biotechnology and bioengineering.

[2]  L. Melo,et al.  Biofilms--science and technology , 1992 .

[3]  K. Cooksey.  Extracellular Polymers in Biofilms , 1992 .

[4]  Hans-Curt Flemming,et al.  Sorption sites in biofilms , 1995 .

[5]  N. Blumenkrantz,et al.  New method for quantitative determination of uronic acids. , 1973, Analytical biochemistry.

[6]  A. Axelsson,et al.  Diffusion in gels containing immobilized cells: A critical review , 1991, Biotechnology and bioengineering.

[7]  V. Lazarova,et al.  Biofilm characterization and activity analysis in water and wastewater treatment , 1995 .

[8]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.

[9]  Per Halkjær Nielsen,et al.  Conceptual Model for Production and Composition of Exopolymers in Biofilms , 1997 .

[10]  W. Borchard,et al.  Investigations of the Complex Shear Modulus and the Optical Rotation in the System Gelatin-Water During the Thermoreversible Gelation Process , 1989 .

[11]  C. Slichter.  Principles of magnetic resonance , 1963 .

[12]  S. Wuertz,et al.  Sorption Properties of Biofilms , 1998 .

[13]  B. Christensen.  The role of extracellular polysaccharides in biofilms , 1989 .

[14]  E. Purcell,et al.  Effects of Diffusion on Free Precession in Nuclear Magnetic Resonance Experiments , 1954 .

[15]  F. Smith,et al.  COLORIMETRIC METHOD FOR DETER-MINATION OF SUGAR AND RELATED SUBSTANCE , 1956 .

[16]  J. Wingender,et al.  Characterization of mucoid Pseudomonas aeruginosa strains isolated from technical water systems. , 1995, The Journal of applied bacteriology.

[17]  K. Müller,et al.  Deuteron N.M.R. relaxation studies of phospholipid membranes , 1988 .