Bacterial Adhesion to Protein-Coated Hydrogels

Extended wear soft contact lenses have been implicated in the increased occurrence of corneal bacterial infections. This research investigated the effects of polymer chemistry, water content, and pre-sorbed proteins upon the adherence of Pseudomonas aeruginosa to model hydrogels with chemistries similar to those of extended wear soft contact lenses. The hydrogels were exposed to washed suspensions of R aeruginosa in a laminar flow cell. Albumin, fibrinogen, desialylated fibrinogen, or mucin were deposited on the hydrogels before exposure to the bacteria. Results showed that with or without protein pre-exposure, bacterial adhesion decreased as water content increased. In the presence of the sorbed protein, the number of adherent bacteria increased by about 45%, and all four proteins caused similar increases in adhesion. Bacterial adhesion was not significantly influenced by the presence of sialic acid residues in the pre-sorbed protein.

[1]  E. C. Poggio,et al.  The relative risk of ulcerative keratitis among users of daily-wear and extended-wear soft contact lenses. A case-control study. Microbial Keratitis Study Group. , 1989, The New England journal of medicine.

[2]  H. Busscher,et al.  Adhesion of oral streptococci from a flowing suspension to uncoated and albumin-coated surfaces. , 1987, Journal of general microbiology.

[3]  M. Devleeschouwer,et al.  Prevention of the adhesion of Pseudomonas aeruginosa to human buccal epithelial cells , 1989 .

[4]  H. Busscher,et al.  Effect of Zeta Potential and Surface Energy on Bacterial Adhesion to Uncoated and Saliva-coated Human Enamel and Dentin , 1988, Journal of dental research.

[5]  L. Hazlett,et al.  In vivo identification of sialic acid as the ocular receptor for Pseudomonas aeruginosa , 1986, Infection and immunity.

[6]  Secretory IgA adsorption and oral streptococcal adhesion to human enamel and artificial solid substrata with various surface free energies. , 1991, Journal of biomaterials science. Polymer edition.

[7]  M. Refojo,et al.  Pseudomonas attachment to new hydrogel contact lenses. , 1987, Archives of ophthalmology.

[8]  E J Cohen,et al.  Corneal ulcers associated with contact lens wear. , 1984, Archives of ophthalmology.

[9]  J. Feijen,et al.  Adhesion of coagulase-negative staphylococci to methacrylate polymers and copolymers. , 1986, Journal of biomedical materials research.

[10]  F. Stapleton,et al.  Nonulcerative complications of contact lens wear. Relative risks for different lens types. , 1992, Archives of ophthalmology.

[11]  Stuart L. Cooper,et al.  Protein adsorption on polymeric biomaterials I. Adsorption isotherms , 1988 .

[12]  W. Pitt,et al.  Bacterial adhesion to poly(HEMA)-based hydrogels. , 1993, Journal of biomedical materials research.

[13]  D. Frazer,et al.  Risks of keratitis and patterns of use with disposable contact lenses. , 1992, Archives of ophthalmology.

[14]  R. D. Stulting,et al.  Corneal ulcer and adverse reaction rates in premarket contact lens studies. , 1991, American journal of ophthalmology.

[15]  S. Klotz,et al.  Contact lens wear enhances adherence of Pseudomonas aeruginosa and binding of lectins to the cornea. , 1990, Cornea.

[16]  R. Ramphal,et al.  Adherence of Pseudomonas aeruginosa to the injured cornea: a step in the pathogenesis of corneal infections. , 1981, Annals of ophthalmology.

[17]  R. Ramphal,et al.  Adherence of mucoid and nonmucoid Pseudomonas aeruginosa to acid-injured tracheal epithelium , 1983, Infection and Immunity.

[18]  Q. Myrvik,et al.  Extended-wear lenses, biofilm, and bacterial adhesion. , 1987, Archives of ophthalmology.

[19]  W. Pitt,et al.  Air-water interface displaces adsorbed bacteria. , 1993, Biomaterials.

[20]  S. Klotz,et al.  Contact lens surface deposits increase the adhesion of Pseudomonas aeruginosa. , 1990, Current eye research.

[21]  R. Lyman Ott.,et al.  An introduction to statistical methods and data analysis , 1977 .

[22]  S. Cooper,et al.  Protein adsorption on polymeric biomaterials: II. Adsorption kinetics , 1988 .

[23]  G. Stern,et al.  The effect of enzymatic contact lens cleaning on adherence of Pseudomonas aeruginosa to soft contact lenses. , 1987, Ophthalmology.

[24]  T. Díaz-Mauriño,et al.  Desialylation of fibrinogen with neuraminidase. Kinetic and clotting studies. , 1982, Thrombosis research.

[25]  N. Topham,et al.  Enhanced bacterial adhesion on surfaces pretreated with fibrinogen and fibronectin. , 1988, ASAIO transactions.

[26]  G. Stern,et al.  The pathogenesis of contact lens-associated Pseudomonas aeruginosa corneal ulceration. II. An animal model. , 1986, Cornea.

[27]  L. Hazlett,et al.  Characterization of Pseudomonas aeruginosa adherence to mouse corneas in organ culture , 1990, Infection and immunity.

[28]  A. Gristina,et al.  Staphylococcal adhesion to collagen in intra-articular sepsis. , 1988, Biomaterials.

[29]  G. Stern,et al.  The interaction between Pseudomonas aeruginosa and the corneal epithelium. An electron microscopic study. , 1985, Archives of ophthalmology.