Inheritance of resistance to Aspergillus ear rot and aflatoxin production of corn from tex6.

ABSTRACT The inheritance of resistance to Aspergillus ear rot and aflatoxin production in corn (Zea mays L.) caused by Aspergillus flavus was studied in progeny derived from crosses between the resistant corn inbred cv. Tex6 and susceptible inbred cvs. B73 and Mo17. From 1994 to 1996, plant generations included were the P(1) (susceptible B73 or Mo17), P(2) (resistant Tex6), F(1), F(2), F(3), BCP(1), BCP(1)-selfed, and BCP(2). The BCP(2)-selfed generation was added in 1995 and 1996 for the B73 x Tex6 cross. Primary ears were pinboard inoculated and evaluated for Aspergillus ear rot severity. F(1) means deviated from the midparent value toward resistance for aflatoxin production and toward susceptibility for ear rot in both crosses. Analyses of generation means indicate that additive gene action was most important in the resistance to both ear rot and aflatoxin production in the B73 x Tex6 cross. Mo17 was somewhat resistant to both traits, so resistance from Tex6 was not well defined in this cross. Broad-sense heritabilities for ear rot and aflatoxin production were 58 and 63% for Mo17 x Tex6, and 66 and 65% for B73 x Tex6. Narrow-sense heritabilities for ear rot and aflatoxin production were 39 and 45% for B73 x Tex6. It is estimated that one cycle of selection for resistance within B73 x Tex6 F(3) families would reduce the percentage of ear rot severity by 8.5% and aflatoxin concentration by 19 ng/g.

[1]  N. W. Widstrom,et al.  Ear Resistance of Maize Inbreds to Field Aflatoxin Contamination 1 , 1984 .

[2]  B. Hayman.  The separation of epistatic from additive and dominance variation in generation means , 1958, Heredity.

[3]  K. W. Campbell,et al.  Inheritance of Resistance to Aflatoxin Production in the Cross Between Corn Inbreds B73 and LB31. , 1997, Phytopathology.

[4]  P. Hamilton,et al.  PLANTING DATE HARVEST DATE AND IRRIGATION EFFECTS ON INFECTION AND AFLA TOXIN PRODUCTION BY ASPERGILLUS FLAVUS IN FIELD CORN , 1981 .

[5]  E. Gamble.  GENE EFFECTS IN CORN (ZEA MAYS L.): I. SEPARATION AND RELATIVE IMPORTANCE OF GENE EFFECTS FOR YIELD , 1962 .

[6]  K. W. Campbell,et al.  Evaluation of corn genotypes for resistance to Aspergillus ear rot, kernel infection, and aflatoxin production , 1995 .

[7]  J. Irwin,et al.  Independent Inheritance of Genes Conditioning Resistance to Phytophthora Root Rot from Diploid and Tetraploid Alfalfa 1 , 1987 .

[8]  G. Payne,et al.  Corn Seed Proteins Inhibitory to Aspergillus flavus and Aflatoxin Biosynthesis. , 1997, Phytopathology.

[9]  Trevathan Le,et al.  Effect of four inoculation techniques on infection and aflatoxin concentration of resistant and susceptible corn hybrids inoculated with Aspergillus flavus. , 1986 .

[10]  A. Hallauer,et al.  Quantitative Genetics in Maize Breeding , 1981 .

[11]  Michael J. Kearsey,et al.  Genetical Analysis of Quantitative Traits , 2020 .

[12]  John Milton Poehlman,et al.  Breeding Field Crops , 1959 .

[13]  K. W. Campbell,et al.  Determination of resistance to aflatoxin production in maize kernels and detection of fungal colonization using an Aspergillus flavus transformant expressing Escherichia coli β-glucuronidase. , 1995 .

[14]  K. W. Campbell,et al.  Inheritance of resistance to Aspergillus ear rot and aflatoxin in corn genotypes. , 1995 .

[15]  M. S. Zuber,et al.  Aflatoxin Contamination of Corn Before Harvest: Aspergillus flavus Association with Insects Collected from Developing Ears , 1978 .

[16]  N. W. Widstrom,et al.  Segregation for Resistance to Aflatoxin Contamination among Seeds on an Ear of Hybrid Maize , 1987 .

[17]  J. R. Wallin,et al.  Genetic Control of Aflatoxin Production in Maize , 1987 .

[18]  T. Cleveland,et al.  Combining Ability for Resistance to Field Aflatoxin Accumulation in Maize Grain , 1992 .

[19]  G. Payne,et al.  Aflatoxin in maize , 1992 .

[20]  K. W. Campbell,et al.  Sources of resistance in F1 corn hybrids to ear rot caused by Aspergillus flavus , 1993 .

[21]  N. W. Widstrom.  The Role of Insects and Other Plant Pests in Aflatoxin Contamination of Corn, Cotton, and Peanuts—A Review , 1979 .

[22]  J. Widholm,et al.  β-l,3-Glucanase and Resistance to Aspergillus flavus Infection in Maize , 1998 .

[23]  M. S. Zuber,et al.  Aflatoxin Production in Aspergillus flavus Inoculated Ears of Corn Grown at Diverse Locations , 1975 .

[24]  M. S. Zuber.  Aflatoxin B1Production in an Eight-Line Diallel of Zea mays infected with Aspergillus flavus , 1978 .

[25]  T. Cleveland,et al.  Resistance to Aspergillus flavus in Corn Kernels Is Associated with a 14-kDa Protein. , 1998, Phytopathology.

[26]  G. E. Scott,et al.  Sources of Resistance in Maize to Kernel Infection by Aspergillus flavus in the Field , 1988 .