Glucose nanosensors based on redox polymer/glucose oxidase modified carbon fiber nanoelectrodes.

This paper describes glucose nanosensors based on the co-electrodeposition of a poly(vinylimidazole) complex of [Os(bpy)(2)Cl](+/2+) and glucose oxidase (GOD) on a low-noise carbon fiber nanoelectrodes (CFNE). The SEM image shows that the osmium redox polymer/enzyme composite film is uniform. The film modified CFNE exhibits the classical features of a kinetically fast redox couple bound to the electrode surface. A strong and stable electrocatalytic current is observed in the presence of glucose. Under the optimal experimental conditions, the nanosensor offers a highly sensitive and rapid response to glucose at an operating potential of 0.22V. A wide linear dynamic rang of 0.01-15mM range was achieved with a detection limit of 0.004mM. Compared with the conventional gold electrode, the nanosensor possessed higher sensitivity and longer stability. Successful attempts were made in real time monitoring rabbit blood glucose levels.

[1]  R. Wightman,et al.  Simultaneous detection of catecholamine exocytosis and Ca2+ release from single bovine chromaffin cells using a dual microsensor. , 1998, Analytical chemistry.

[2]  C. Danilowicz,et al.  An Os(byp)2ClPyCH2NHPoly(allylamine) hydrogel mediator for enzyme wiring at electrodes , 1998 .

[3]  E. F. Bowden,et al.  Enzyme-Substrate Kinetics of Adsorbed Cytochrome c Peroxidase on Pyrolytic Graphite Electrodes , 1994 .

[4]  Hongyuan Chen,et al.  Properties of poly-β-aminoanthraquinone modified carbon fiber electrode as a basis for hemoglobin biosensors , 1996 .

[5]  W. Zhang,et al.  Fabrication, characterization, and potential application of carbon fiber cone nanometer-size electrodes. , 1996, Analytical chemistry.

[6]  Adam Heller,et al.  Long tethers binding redox centers to polymer backbones enhance electron transport in enzyme "Wiring" hydrogels. , 2003, Journal of the American Chemical Society.

[7]  Wei-Hua Huang,et al.  Transport, location, and quantal release monitoring of single cells on a microfluidic device. , 2004, Analytical chemistry.

[8]  Zhiqiang Gao,et al.  An amperometric biosensor for glucose based on electrodeposited redox polymer/glucose oxidase film on a gold electrode. , 2003, Analytical sciences : the international journal of the Japan Society for Analytical Chemistry.

[9]  R. Murray,et al.  Solid-state linear sweep voltammetry: a probe of diffusion in thin films of polymer ion conductors on microdisk electrodes , 1987 .

[10]  M Ohwa,et al.  Design of a stable charge transfer complex electrode for a third-generation amperometric glucose sensor. , 1996, Analytical chemistry.

[11]  Baohong Liu,et al.  Al2O3 sol–gel derived amperometric biosensor for glucose , 1999 .

[12]  C. Lowe,et al.  Immobilization of glucose oxidase in ferrocene-modified pyrrole polymers. , 1988, Analytical chemistry.

[13]  Adam Heller,et al.  Electrical Connection of Enzyme Redox Centers to Electrodes , 1992 .

[14]  C Hong-Yuan,et al.  Amperometric glucose sensor based on coimmobilization of glucose oxidase and Poly(p-phenylenediamine) at a platinum microdisk electrode. , 2000, Analytical biochemistry.

[15]  H. S. Kim,et al.  Affinity biosensor for avidin using a double functionalized dendrimer monolayer on a gold electrode. , 2000, Analytical biochemistry.

[16]  C. Danilowicz,et al.  Layer-by-layer self-assembly of glucose oxidase and Os(Bpy)2CIPyCH2NH-poly(allylamine) bioelectrode. , 2001, Analytical chemistry.

[17]  J. Zen,et al.  A glucose biosensor employing a stable artificial peroxidase based on ruthenium purple anchored cinder. , 2003, Analytical chemistry.

[18]  Adam Heller,et al.  Screen printing of nucleic acid detecting carbon electrodes. , 2002, Analytical chemistry.

[19]  R. Wightman,et al.  Dopamine transport into a single cell in a picoliter vial. , 2002, Analytical chemistry.

[20]  Wei-Hua Huang,et al.  Carbon fiber nanoelectrodes modified by single-walled carbon nanotubes. , 2003, Analytical chemistry.

[21]  G. S. Wilson,et al.  Pulsed amperometric detection of glucose in biological fluids at a surface-modified gold electrode. , 1989, Analytical chemistry.

[22]  R. Wightman,et al.  Response of microvoltammetric electrodes to homogeneous catalytic and slow heterogeneous charge-transfer reactions , 1980 .

[23]  Andrew G. Ewing,et al.  Characterization of submicron-sized carbon electrodes insulated with a phenol-allylphenol copolymer , 1992 .

[24]  A. Ewing,et al.  Amperometric monitoring of stimulated catecholamine release from rat pheochromocytoma (PC12) cells at the zeptomole level. , 1994, Analytical chemistry.

[25]  Katz,et al.  Integration of Layered Redox Proteins and Conductive Supports for Bioelectronic Applications. , 2000, Angewandte Chemie.

[26]  A Heller,et al.  Amperometric glucose microelectrodes prepared through immobilization of glucose oxidase in redox hydrogels. , 1991, Analytical chemistry.

[27]  Ursula E. Spichiger,et al.  Glucose Nanosensor Based on Prussian-Blue Modified Carbon-Fiber Cone Nanoelectrode and an Integrated Reference Electrode , 1999 .

[28]  D. Pang,et al.  A method for the fabrication of low-noise carbon fiber nanoelectrodes. , 2001, Analytical chemistry.