In vitro maturation of collared peccary (Pecari tajacu) oocytes after different incubation times

ABSTRACT: Oocyte in vitro maturation (IVM) is the first step of the in vitro reproductive technologies that enables mature oocytes to be generated ex vivo and after used for embryo production. In this sense, the establishment of culture environment, as oocyte incubation time, is essential for the success of the IVM. Therefore, the study was carried out to investigate the relationship between the meiotic potential and the IVM times of collared peccary oocytes, wild mammals of great commercial and ecological interest. Thus, ovaries were collected of females derived from captivity and transported to the laboratory within 1 hour of slaughtering. The oocytes derived from follicles (3-6mm in diameter) were recovered by aspirated and sliced. Good quality oocytes (evenly granulated cytoplasm with a least one layer of surrounding cumulus cells) were selected and subjected to culture in TCM 199 supplemented with 10µg/mL FSH, 10% FBS and 100µM cysteamine at 38.5°C, 5% CO2 and maximum humidity for 24 or 48 hours. After the incubation period, the nuclear status, the presence of first polar body and the expansion of cumulus cells of oocytes were assessed. The data obtained were analyzed by Fisher exact test (P<0.05). A total of four sessions (2-3 females per session) were performed, resulting in eighteen aspirated and sliced ovaries with normal morphological characteristics. An oocyte recovery rate of about 83.1% (59/71) was obtained with 3.3 oocytes/ovary and 2.3 viable oocytes/ovary. After different incubation times, differences (P<0.05) were observed in 24 and 48 hours for expansion of the cumulus cells (38.1% vs. 100%), presence of first polar body (52.4% vs. 90.5%) and nuclear status in second metaphase (19.0% vs. 76.2%), respectively. In conclusion, 48 hours is suitable time for the in vitro maturation of oocytes derived from collared peccaries when compared to the time of 24 hours, according to the meiotic potential observed. Additional studies should be conducted to improve the quality of the oocyte culture environment, as medium composition, aiming to obtain viable mature oocytes for other in vitro biotechnologies.

[1]  C. Ahuja-Aguirre,et al.  Progesterone and estradiol profiles in different reproductive stages of captive collared peccary (Pecari tajacu) females assessed by fecal metabolites. , 2017, Animal reproduction science.

[2]  M. Nasr-Esfahani,et al.  Interspecies somatic cell nuclear transfer in Asiatic cheetah using nuclei derived from post-mortem frozen tissue in absence of cryo-protectant and in vitro matured domestic cat oocytes. , 2017, Theriogenology.

[3]  S. Hyun,et al.  Effect of zeaxanthin on porcine embryonic development during in vitro maturation. , 2017, Journal of biomedical research.

[4]  T. Takada,et al.  Early germinal vesicle breakdown is a predictor of high preimplantation developmental competent oocytes in mice , 2016, Zygote.

[5]  S. Ebeling,et al.  Effect of Follicle Size on In Vitro Maturation of Pre-Pubertal Porcine Cumulus Oocyte Complexes. , 2016, Reproduction in domestic animals = Zuchthygiene.

[6]  M. S. Ferraz,et al.  Técnica de fatiamento do ovário para obtenção de oócitos em cutias (Dasyprocta prymnolopha) , 2016 .

[7]  P. Pawlak,et al.  Mitochondria and mitochondrial DNA in porcine oocytes and cumulus cells--A search for developmental competence marker. , 2016, Mitochondrion.

[8]  P. Papa,et al.  Morphology and vascularization of the corpus luteum of peccaries (Pecari tajacu, Linnaeus, 1758) throughout the estrous cycle , 2016 .

[9]  M. Ješeta,et al.  Energy Status Characteristics of Porcine Oocytes During In Vitro Maturation is Influenced by Their Meiotic Competence. , 2015, Reproduction in domestic animals = Zuchthygiene.

[10]  A. Van Soom,et al.  Increasing the cAMP concentration during in vitro maturation of pig oocytes improves cumulus maturation and subsequent fertilization in vitro. , 2015, Theriogenology.

[11]  Yong-chao Jiang,et al.  Lysophosphatidic acid improves porcine oocyte maturation and embryo development in vitro , 2015, Molecular reproduction and development.

[12]  L. B. Campos,et al.  Estrus cycle monitoring of captive collared peccaries (Pecari tajacu) in semiarid conditions1 , 2015 .

[13]  M. F. Oliveira,et al.  Monitoramento de ciclo estral em catetos (Pecari tajacu) criados em cativeiro em condições semiáridas , 2014 .

[14]  S. Hyun,et al.  Developmental competence of IVM pig oocytes after SCNT in relation to the shrinkage pattern induced by hyperosmotic treatment. , 2014, Theriogenology.

[15]  M. C. Schoeman,et al.  Ecological correlates of small mammal assemblage structure at different spatial scales in the savannah biome of South Africa , 2014 .

[16]  D. Eaton,et al.  The effects of deforestation on white-lipped peccary (Tayassu pecari) home range in the southern Pantanal , 2014 .

[17]  C. Grupen.  The evolution of porcine embryo in vitro production. , 2014, Theriogenology.

[18]  M. Shamiah,et al.  IN VITRO MATURATION, FERTILIZATION AND DEVELOPMENT OF PREPUBERTAL AND MATURE BUFFALO OOCYTES , 2014 .

[19]  A. Rodrigues,et al.  Morphological Characterization of the Ovarian Preantral Follicle Population of Collared Peccaries (Tayassu tajacu Linnaeus, 1758) , 2013, Anatomia, histologia, embryologia.

[20]  D. Guimarães,et al.  Ovarian folliculogenesis in collared peccary, Pecari tajacu (Artiodactyla: Tayassuidae). , 2012, Revista de biologia tropical.

[21]  Alexandre Rossetto Garcia,et al.  Aspectos reprodutivos de caititus (Tayassu tajacu) , 2009 .

[22]  J. Jarazo,et al.  A unique method to produce transgenic embryos in ovine, porcine, feline, bovine and equine species. , 2008, Reproduction, fertility, and development.

[23]  R. Abdullah,et al.  IN VITRO MATURATION OF OOCYTES WITH SPECIAL REFERENCE TO GOAT: A REVIEW , 2008 .

[24]  F. G. Basiouni.  Follicular Wave Pattern, Folliculogenesis and Assisted Reproductive Techniques in the Non-pregnant Female Dromedary Camel (Camelus dromedarius): A Review , 2007 .

[25]  F. Richard,et al.  Contribution of the oocyte to embryo quality. , 2006, Theriogenology.

[26]  F. López-Gatius,et al.  Integrating ultrasonography within the reproductive management of the collared peccary (Tayassu tajacu). , 2005, Theriogenology.

[27]  F. Jori,et al.  A large-scale commercial farming of collared peccary (Tayassu tajacu) in north-eastern Brazil , 2004 .

[28]  P. Palta,et al.  Folliculogenesis in buffalo (Bubalus bubalis): a review. , 2002, Reproduction, fertility, and development.

[29]  S. Stice,et al.  Utility of Rapidly Matured Oocytes as Recipients for Production of Cloned Embryos from Somatic Cells in the Pig1 , 2002, Biology of reproduction.

[30]  L. R. Abeydeera.  In vitro production of embryos in swine. , 2002, Theriogenology.

[31]  R. Prather,et al.  Cytoplasmic changes in relation to nuclear maturation and early embryo developmental potential of porcine oocytes: Effects of gonadotropins, cumulus cells, follicular size, and protein synthesis inhibition * , 2001, Molecular reproduction and development.

[32]  S. Meczyński.  Morphohistological structure of female genital organs in sousliks. , 1974, Acta theriologica.