Isolation of Y chromosome-specific microsatellites in the horse and cross-species amplification in the genus Equus.

Y chromosome polymorphisms such as microsatellites or single nucleotide polymorphisms represent a paternal counterpart to mitochondrial DNA (mtDNA) for evolutionary and phylogeographic studies. The use of Y chromosome haplotyping in natural populations of species other than humans is still hindered by the lack of sequence information necessary for polymorphism screening. Here we used representational difference analysis (RDA) followed by a screen of a bacterial artificial chromosome (BAC) library for repetitive sequences to obtain polymorphic Y-chromosomal markers. The procedure was performed for the domestic horse (Equus caballus) and we report the first six Y-chromosomal microsatellite markers for this species. Three markers were also useful for haplotyping taxa of the zebra/ass lineage. Y-chromosomal microsatellite markers show a single haplotype in the domestic horse, whereas notable variation has been observed in the other members of the genus Equus.

[1]  A. Jeffreys,et al.  Isolation of human simple repeat loci by hybridization selection. , 1994, Human molecular genetics.

[2]  A Sajantila,et al.  Characteristics and frequency of germline mutations at microsatellite loci from the human Y chromosome, as revealed by direct observation in father/son pairs. , 2000, American journal of human genetics.

[3]  M. Wigler,et al.  Cloning the differences between two complex genomes , 1993, Science.

[4]  R. W. Davis,et al.  Population genetic implications from sequence variation in four Y chromosome genes. , 2000, Proceedings of the National Academy of Sciences of the United States of America.

[5]  G. Brem,et al.  Base substitutions in the sequences flanking microsatellite markers HMS3 and ASB2 interfere with parentage testing in the Lipizzan horse. , 2001, Animal genetics.

[6]  R. Wayne,et al.  Widespread origins of domestic horse lineages. , 2001, Science.

[7]  Han N. Lim,et al.  A survey of equid mitochondrial DNA: Implications for the evolution, genetic diversity and conservation of Equus , 2000, Conservation Genetics.

[8]  G. Brem,et al.  Polymorphic DNA microsatellites in the domestic pigeon, Columba livia var. domestica , 2000, Molecular ecology.

[9]  J. Talag,et al.  Characterization of transposable elements in the genome of rice (Oryza sativa L.) using Representational Difference Analysis (RDA) , 2002, Molecular Genetics and Genomics.

[10]  E. Cribiu,et al.  Construction of a horse BAC library and cytogenetical assignment of 20 type I and type II markers , 1998, Mammalian Genome.

[11]  S. Ewart,et al.  Polymorphism identification within 50 equine gene-specific sequence tagged sites. , 2001, Animal genetics.

[12]  M. Levine.  Botai and the Origins of Horse Domestication , 1999 .

[13]  D. Page,et al.  The human Y chromosome: overlapping DNA clones spanning the euchromatic region. , 1992, Science.

[14]  M. Hurles,et al.  Mitochondrial DNA and the origins of the domestic horse , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[15]  M. McClelland,et al.  DNA rehybridization during PCR: the 'Cot effect' and its consequences. , 1996, Nucleic acids research.

[16]  M. Hurles,et al.  Haploid chromosomes in molecular ecology: lessons from the human Y , 2001, Molecular ecology.

[17]  C. Beattie,et al.  Development of a bovine X chromosome linkage group and painting probes to assess cattle, sheep, and goat X chromosome segment homologies. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[18]  L. S. Cram,et al.  The mouse Y chromosome: enrichment, sizing, and cloning by bivariate flow cytometry. , 1998, Genomics.

[19]  F. Sato,et al.  Linear SRY transcript in equine testis. , 1999, The Journal of veterinary medical science.

[20]  E. Dunnington.  Przewalski's horse — The history and biology of an endangered species , 1995 .

[21]  R J Mitchell,et al.  The geographic distribution of human Y chromosome variation. , 1997, Genetics.