A Novel Nonionic, Multi-Surfactant System and Separation Method for the Synthesis of Active Carbonic Anhydrase Nanoparticles

This paper describes a new synthesis and separation method of nanoparticles (NPs) using a non-toxic, non-ionic surfactant systems. The purification steps did not use ethanol or acetone. Results indicate that the wild type bovine Carbonic Anhydrase (CA) activity was enhanced almost 4 times more than the CA encapsulated NPs fabricated by the traditional method. The NPs have are more hydrophilic and also have a higher zeta potential. The well dispersed CA PAA NPs with of 10-30 nm in diameter were obtained. This work also demonstrates a universal method for immobilizing fragile biomacromolecules in NP carriers for biomedical applications.