Auxin regulation of a proton translocating ATPase in pea root plasma membrane vesicles.

Pea root microsomal vesicles have been fractionated on a Dextran step gradient to give three fractions, each of which carries out ATP-dependent proton accumulation as measured by fluorescence quenching of quinacrine. The fraction at the 4/6% Dextran interface is enriched in plasma membrane, as determined by UDPG sterol glucosyltransferase and vanadate-inhibited ATPase. The vanadate-sensitive phosphohydrolase is not specific for ATP, has a K(m) of about 0.23 millimolar for MgATP, is only slightly affected by K(+) or Cl(-) and is insensitive to auxin. Proton transport, on the other hand, is more specific for ATP, enhanced by anions (NO(3) (-) > Cl(-)) and has a K(m) of about 0.7 millimolar. Auxins decrease the K(m) to about 0.35 millimolar, with no significant effect on the V(max), while antiauxins or weak acids have no such effect. It appears that auxin has the ability to alter the efficiency of the ATP-driven proton transport.

[1]  A. G. Filoteo,et al.  Regulation of plasma membrane Ca2+ ATPases by lipids of the phosphatidylinositol cycle. , 1984, Biochemical and biophysical research communications.

[2]  D. Morré,et al.  Phosphatidylinositol turnover in isolated soybean membranes stimulated by the synthetic growth hormone 2,4-dichlorophenoxyacetic acid. , 1984, The Journal of biological chemistry.

[3]  H. Sze.  H+‐translocating ATPases of the plasma membrane and tonoplast of plant cells , 1984 .

[4]  R. Addison,et al.  On the subunit composition of the Neurospora plasma membrane H+-ATPase. , 1984, The Journal of biological chemistry.

[5]  R. M. Spanswick,et al.  Effects of vanadate on the plasma membrane ATPase of red beet and corn. , 1984, Plant physiology.

[6]  C. Romieu,et al.  Local Ionic Environment of Plant Membranes: Effects on Membrane Functions , 1984 .

[7]  E. Racker,et al.  Effect of lipid composition on the calcium/adenosine 5'-triphosphate coupling ratio of the Ca2+-ATPase of sarcoplasmic reticulum. , 1984, Biochemistry.

[8]  R. Serrano.  Purification and reconstitution of the proton-pumping ATPase of fungal and plant plasma membranes. , 1983, Archives of biochemistry and biophysics.

[9]  J. Dubacq,et al.  In Situ and IAA-Induced Cell Elongation is Correlated to the Oleoyl Phosphatidylcholine Content along the Vigna radiata Hypocotyl , 1983 .

[10]  M. Evans,et al.  Promotion of growth and hydrogen ion efflux by auxin in roots of maize pretreated with ethylene biosynthesis inhibitors. , 1982, Plant physiology.

[11]  R. Cleland,et al.  Evidence for a Cl-Stimulated MgATPase Proton Pump in Oat Root Membranes. , 1982, Plant physiology.

[12]  L. Taiz,et al.  Vanadate inhibition of auxin-enhanced H secretion and elongation in pea epicotyls and oat coleoptiles. , 1980, Proceedings of the National Academy of Sciences of the United States of America.

[13]  H. Sze.  Nigericin-stimulated ATPase activity in microsomal vesicles of tobacco callus. , 1980, Proceedings of the National Academy of Sciences of the United States of America.

[14]  W. Briggs,et al.  Auxin receptors of maize coleoptile membranes do not have ATPase activity. , 1978, Plant physiology.

[15]  K. Kasamo,et al.  Effect of auxin on Mg++-activated and -inhibited ATPases from mung bean hypocotyls , 1974 .

[16]  R. Bieleski.  Phosphate Pools, Phosphate Transport, and Phosphate Availability , 1973 .

[17]  R. Colombo,et al.  Correlation between cell enlargement in pea internode segments and decrease in the pH of the medium of incubation: II. Effects of inhibitors of respiration, oxidative phosphorylation and protein synthesis , 1973 .

[18]  Oliver H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.

[19]  R. E. Mccarty.  H+-ATPases in Oxidative and Photosynthetic Phosphorylation , 1985 .

[20]  J. B. Hanson,et al.  Control of proton efflux from corn root tissue by an injury-sensing mechanism , 1982 .

[21]  R. Cleland.  Auxin and H+-Excretion: The State of Our Knowledge , 1980 .

[22]  P. Benveniste,et al.  Sterol biosynthetic capability of purified membrane fractions from maize coleoptiles , 1978 .

[23]  R. Cleland,et al.  Control of plant cell enlargement by hydrogen ions. , 1977, Current topics in developmental biology.