SUPPLEMENTAL EXPERIMENTAL PROCEDURES

SUPPLEMENTAL EXPERIMENTAL PROCEDURES Plasmid constructions The full-length coding region of human HIF2α or HIF1α genes (gifts from Dr. Richard Bruick and Dr. Amato Giaccia, respectively) was PCR amplified in frame with a forward linker encoding HA tag downstream a NheI restriction site and a reverse linker with a Not1 site downstream cDNA sequence (see supplemental table 3 for oligonucleotide sequences). The amplified PCR fragments and pCMV hygro 2xFLAG expression vector (gift from Dr. Pierre Lutz, IPBS, Toulouse) were digested by Nhe1 and Not1 restriction enzymes, purified and sub-cloned. Fidelity of PCR and identity of the constructs were confirmed by sequencing the inserts.

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