Splenic phagocytic function after partial splenectomy and splenic autotransplantation.

We investigated splenic reticuloendothelial activity after splenic preservation procedures to determine their effect upon the phagocytic function of the spleen. We performed the following procedures in Sprague-Dawley rats: sham laparotomy, total splenectomy, hemisplenectomy, subtotal splenectomy, or total splenectomy with intraperitoneal splenic autotransplantation. At nine weeks after operation, phagocytic function of the spleen was determined by measuring radiocolloid uptake. Mean (+/- SEM) splenic phagocytic indices for sham laparotomy (41.2 +/- 2.9), hemisplenectomy (44 +/- 2.9), and subtotal splenectomy (43.2 +/- 5.2) were similar; however, the phagocytic index was reduced markedly after autotransplantation (15.8 +/- 2.2). These data demonstrate that the phagocytic function of the spleen after hemisplenectomy and subtotal splenectomy correlates highly with the weight of the splenic remnant; however, phagocytic function after autotransplantation remains reduced even after accounting for differences in splenic weight.

[1]  I. Chaudry,et al.  Beneficial effects of implanted muscle tissue on pulmonary particulate retention and survival following splenectomy. , 1983, Journal of Trauma.

[2]  K. Sirinek,et al.  Improved survival rate for intraperitoneal autotransplantation of the spleen following pneumococcal pneumonia. , 1983, Surgery, gynecology & obstetrics.

[3]  D. Coln,et al.  The Importance of Splenic Blood Flow In Clearing Pneumococcal Orgnanisms , 1982, Annals of surgery.

[4]  J. Church,et al.  Antibody responses after splenectomy and splenic autoimplantation in rats. , 1981, The Journal of surgical research.

[5]  D. Cooney,et al.  Comparative methods of splenic preservation. , 1981, Journal of pediatric surgery.

[6]  R. Strunk,et al.  Humoral immunity in experimental hyposplenism. , 1978, Surgery.

[7]  J. Winkelstein,et al.  Lack of Protective Effect of Autotransplanted Splenic Tissue to Pneumococcal Challenge , 1978 .

[8]  V. Likhite.  Opsonin and leukophilic γ globulin in chronically splenectomised rats with and without heterotropic autotransplanted splenic tissue , 1975, Nature.

[9]  I. Clarét,et al.  Immunological studies in the postsplenectomy syndrome. , 1975, Journal of pediatric surgery.

[10]  L. Weiss,et al.  A scanning electron microscopic study of the spleen. , 1974, Blood.

[11]  M. Tavassoli,et al.  Studies on regeneration of heterotopic splenic autotransplants. , 1973, Blood.

[12]  T. Saba.  Physiology and physiopathology of the reticuloendothelial system. , 1970, Archives of internal medicine.

[13]  H. Stern,et al.  Preparation, distribution and utilization of technetium-99m-sulfur colloid. , 1966, Journal of nuclear medicine : official publication, Society of Nuclear Medicine.

[14]  H. Jacob,et al.  REGULATION OF SPLEEN GROWTH AND SEQUESTERING FUNCTION. , 1963, The Journal of clinical investigation.

[15]  M. Witte,et al.  Critical splenic mass for survival from experimental pneumococcemia. , 1980, The Journal of surgical research.

[16]  Malangoni Ma,et al.  Blunt splenic trauma: a reassessment of surgical therapy based on laboratory and clinical observations. , 1980 .

[17]  Barnhart Mi,et al.  Structural physiology of the human spleen. , 1979 .