Studies on pharmacokinetics of pharmacokinetic-markers in Huanglianjiedu Decoction to cerebral ischemia reperfusion model mice

Aim The purpose of this study was to investigate the pharmacokinetics of harmacokinetic-markers, berberine (BR), baicalin (BC) and jasminoidin (JD), in Huanglianjiedu Decoction. The clinical medication program of Huanglianjiedu Decoction would be determined, by detecting the differences of the drug concentration between normal and cerebral ischemia reperfusion model mice, and the concentration dependent relationship between blood plasma and brain tissue of each component. Methods The HPLC method was established for determining three effective components in blood plasma and brain tissue of mice. The mice are divided into two groups, the normal and cerebral ischemia reperfusion model. Huanglianjiedu decoction was administered orally. At different time spot, the eyeballs of mice were removed to collect blood, and the brain tissues were separated. Methanol was used to precipitate protein and the clear supernatant liquid was obtained by centrifugation. The pharmacokinetic parameters were calculated with pharmacokinetic software 3p97. The t-test was used to analyze the differences of the data. Results The plasma drug concentration-time curve characteristics of BH, BC and JD in Huanglianjiedu Decoction in normal and cerebral ischemia reperfusion model mice were fitted for two-compartment model. The brain tissue concentration-time curves of BH, BC and JD in Huanglianjiedu Decoction in normal and cerebral ischemia reperfusion model mice were also fitted by one -compartment model. The concentrations of BH, BC and JD in absorption and elimination phase showed strong correlation between plasma and brain tissue in normal and cerebral ischemia reperfusion model mice. Conclusion The pharmacokinetics parameters of BH, BC and JD in Huanglianjiedu Decoction in model mice indicate the faster absorption and elimination compared with normal mice. Besides, the strong correlation of concentration between plasma and brain tissue of BH, BC and JD, reveals that the variation of concentrations in plasma can reflect the variation of concentrations in brain tissue.