Prokaryotic Expression and Purification of LscW Protein of Lawsonia intracellularis for biomedical engineering

In order to study the LscW of Lawsonia intracellularis, the prokaryotic expression vector of LscW protein was constructed. Two primers were designed in this experiment. The 1197 bp lscW gene was amplified from L. intracellularis B3903 strain by PCR and inserted into pCold expression vector to construct recombinant plasmid pCold/lscW, which was transformed into BL21 competent cells. The positive clones were induced by IPTG, purified by protein purification column, and confirmed by SDS-PAGE gel electrophoresis. The LscW was expressed in the supernatant and purified to obtain a single target protein band.