PatJAZ6 Acts as a Repressor Regulating JA-Induced Biosynthesis of Patchouli Alcohol in Pogostemon Cablin

The JASMONATE ZIM DOMAIN (JAZ) proteins act as negative regulators in the jasmonic acid (JA) signaling pathways of plants, and these proteins have been reported to play key roles in plant secondary metabolism mediated by JA. In this study, we firstly isolated one JAZ from P. cablin, PatJAZ6, which was characterized and revealed based on multiple alignments and a phylogenic tree analysis. The result of subcellular localization indicated that the PatJAZ6 protein was located in the nucleus of plant protoplasts. The expression level of PatJAZ6 was significantly induced by the methyl jasmonate (MeJA). Furthermore, by means of yeast two-hybrid screening, we identified two transcription factors that interact with the PatJAZ6, the PatMYC2b1 and PatMYC2b2. Virus-induced gene silencing (VIGS) of PatJAZ6 caused a decrease in expression abundance, resulting in a significant increase in the accumulation of patchouli alcohol. Moreover, we overexpressed PatJAZ6 in P. cablin, which down-regulated the patchoulol synthase expression, and then suppressed the biosynthesis of patchouli alcohol. The results demonstrate that PatJAZ6 probably acts as a repressor in the regulation of patchouli alcohol biosynthesis, contributed to a model proposed for the potential JA signaling pathway in P. cablin.

[1]  Xiuzhen Chen,et al.  Comparative iTRAQ-based proteomic analysis provides insight into a complex regulatory network of Pogostemon cablin in response to exogenous MeJA and Ethrel , 2019, Industrial Crops and Products.

[2]  Hiroto Hirano,et al.  Virus-induced gene silencing in chili pepper by apple latent spherical virus vector. , 2019, Journal of virological methods.

[3]  H. Goh,et al.  Methyl jasmonate-induced compositional changes of volatile organic compounds in Polygonum minus leaves. , 2019, Journal of plant physiology.

[4]  Fuguang Li,et al.  The cotton GhWIN2 gene activates the cuticle biosynthesis pathway and influences the salicylic and jasmonic acid biosynthesis pathways , 2019, BMC Plant Biology.

[5]  D. Wei,et al.  Significantly enhanced production of patchoulol in metabolically engineered Saccharomyces cerevisiae. , 2019, Journal of agricultural and food chemistry.

[6]  Yongfeng Yang,et al.  Tobacco transcription repressors NtJAZ: Potential involvement in abiotic stress response and glandular trichome induction. , 2019, Plant physiology and biochemistry : PPB.

[7]  Hai Zheng,et al.  Molecular identification and expression of sesquiterpene pathway genes responsible for patchoulol biosynthesis and regulation in Pogostemon cablin , 2019, Botanical Studies.

[8]  Xiuzhen Chen,et al.  Full-length transcriptome sequencing and methyl jasmonate-induced expression profile analysis of genes related to patchoulol biosynthesis and regulation in Pogostemon cablin , 2019, BMC Plant Biology.

[9]  D. Lian,et al.  Unraveling the Novel Protective Effect of Patchouli Alcohol Against Helicobacter pylori-Induced Gastritis: Insights Into the Molecular Mechanism in vitro and in vivo , 2018, Front. Pharmacol..

[10]  G. Howe,et al.  JAZ repressors of metabolic defense promote growth and reproductive fitness in Arabidopsis , 2018, Proceedings of the National Academy of Sciences.

[11]  Dongfeng Yang,et al.  Transcriptional Profiles of SmWRKY Family Genes and Their Putative Roles in the Biosynthesis of Tanshinone and Phenolic Acids in Salvia miltiorrhiza , 2018, International journal of molecular sciences.

[12]  Longfu Zhu,et al.  GhJAZ2 attenuates cotton resistance to biotic stresses via the inhibition of the transcriptional activity of GhbHLH171. , 2018, Molecular plant pathology.

[13]  Juane Dong,et al.  SmJAZ8 acts as a core repressor regulating JA-induced biosynthesis of salvianolic acids and tanshinones in Salvia miltiorrhiza hairy roots , 2018, Journal of experimental botany.

[14]  Cheng Peng,et al.  Patchouli alcohol isolated from Pogostemon cablin mediates endothelium-independent vasorelaxation by blockade of Ca2+ channels in rat isolated thoracic aorta. , 2017, Journal of ethnopharmacology.

[15]  I. Baldwin,et al.  Flower-specific jasmonate signaling regulates constitutive floral defenses in wild tobacco , 2017, Proceedings of the National Academy of Sciences.

[16]  P. Ni,et al.  Genome-wide identification and characterization of JAZ gene family in upland cotton (Gossypium hirsutum) , 2017, Scientific Reports.

[17]  Cheng Peng,et al.  Availability, Pharmaceutics, Security, Pharmacokinetics, and Pharmacological Activities of Patchouli Alcohol , 2017, Evidence-based complementary and alternative medicine : eCAM.

[18]  Z. Su,et al.  In Vitro and In Vivo Antibacterial Activities of Patchouli Alcohol, a Naturally Occurring Tricyclic Sesquiterpene, against Helicobacter pylori Infection , 2017, Antimicrobial Agents and Chemotherapy.

[19]  K. Tang,et al.  HOMEODOMAIN PROTEIN 1 is required for jasmonate-mediated glandular trichome initiation in Artemisia annua. , 2017, The New phytologist.

[20]  Pei Liu,et al.  Effects of MeJA on Arabidopsis metabolome under endogenous JA deficiency , 2016, Scientific Reports.

[21]  J. Memelink,et al.  The basic helix-loop-helix transcription factor BIS2 is essential for monoterpenoid indole alkaloid production in the medicinal plant Catharanthus roseus. , 2016, The Plant journal : for cell and molecular biology.

[22]  M. K. Swamy,et al.  Patchouli (Pogostemon cablin Benth.): Botany, agrotechnology and biotechnological aspects , 2016 .

[23]  G. Stacey,et al.  An Improved Transient Expression System Using Arabidopsis Protoplasts. , 2016, Current protocols in plant biology.

[24]  Shilin Chen,et al.  SmMYC2a and SmMYC2b played similar but irreplaceable roles in regulating the biosynthesis of tanshinones and phenolic acids in Salvia miltiorrhiza , 2016, Scientific Reports.

[25]  Youguo Li,et al.  A JAZ Protein in Astragalus sinicus Interacts with a Leghemoglobin through the TIFY Domain and Is Involved in Nodule Development and Nitrogen Fixation , 2015, PloS one.

[26]  P. Griffin,et al.  Structural basis of JAZ repression of MYC transcription factors in jasmonate signaling , 2015, Nature.

[27]  M. K. Swamy,et al.  A Comprehensive Review on the Phytochemical Constituents and Pharmacological Activities of Pogostemon cablin Benth.: An Aromatic Medicinal Plant of Industrial Importance , 2015, Molecules.

[28]  Y. Toda,et al.  Overexpression of the JAZ factors with mutated jas domains causes pleiotropic defects in rice spikelet development , 2014, Plant signaling & behavior.

[29]  K. Mysore,et al.  Tobacco rattle virus–based virus-induced gene silencing in Nicotiana benthamiana , 2014, Nature Protocols.

[30]  J. Franco-Zorrilla,et al.  bHLH003, bHLH013 and bHLH017 Are New Targets of JAZ Repressors Negatively Regulating JA Responses , 2014, PloS one.

[31]  C. Wasternack,et al.  Jasmonates: biosynthesis, perception, signal transduction and action in plant stress response, growth and development. An update to the 2007 review in Annals of Botany. , 2013, Annals of botany.

[32]  Yitao Wang,et al.  Analysis of Pogostemon cablin from pharmaceutical research to market performances , 2013, Expert opinion on investigational drugs.

[33]  Matthias Lange,et al.  Virus-induced gene silencing (VIGS) in plants: an overview of target species and the virus-derived vector systems. , 2013, Methods in molecular biology.

[34]  Qin Chen,et al.  Over-expression of a novel JAZ family gene from Glycine soja, increases salt and alkali stress tolerance. , 2012, Biochemical and biophysical research communications.

[35]  A. Goossens,et al.  The JAZ Proteins: A Crucial Interface in the Jasmonate Signaling Cascade , 2011, Plant Cell.

[36]  T. Hashimoto,et al.  Tobacco MYC2 regulates jasmonate-inducible nicotine biosynthesis genes directly and by way of the NIC2-locus ERF genes. , 2011, Plant & cell physiology.

[37]  W. Peng,et al.  The Jasmonate-ZIM Domain Proteins Interact with the R2R3-MYB Transcription Factors MYB21 and MYB24 to Affect Jasmonate-Regulated Stamen Development in Arabidopsis[C][W] , 2011, Plant Cell.

[38]  S. Reinbothe,et al.  Plant oxylipins: role of jasmonic acid during programmed cell death, defence and leaf senescence , 2009, The FEBS journal.

[39]  R. Solano,et al.  Plant oxylipins: COI1/JAZs/MYC2 as the core jasmonic acid‐signalling module , 2009, The FEBS journal.

[40]  D. Inzé,et al.  Expression of the Arabidopsis jasmonate signalling repressor JAZ1/TIFY10A is stimulated by auxin , 2009, EMBO reports.

[41]  A. M. Api,et al.  Fragrance material review on patchouli alcohol. , 2008, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[42]  G. Martin,et al.  Applications and advantages of virus-induced gene silencing for gene function studies in plants. , 2004, The Plant journal : for cell and molecular biology.

[43]  R. Solano,et al.  JASMONATE-INSENSITIVE1 Encodes a MYC Transcription Factor Essential to Discriminate between Different Jasmonate-Regulated Defense Responses in Arabidopsis , 2004, The Plant Cell Online.

[44]  S. Dinesh-Kumar,et al.  Virus-induced gene silencing in tomato. , 2002, The Plant journal : for cell and molecular biology.

[45]  D. Baulcombe,et al.  Technical Advance: Tobacco rattle virus as a vector for analysis of gene function by silencing , 2008 .

[46]  M. Zenk,et al.  Jasmonic acid is a signal transducer in elicitor-induced plant cell cultures. , 1992, Proceedings of the National Academy of Sciences of the United States of America.