论文信息 - Kinetic Studies on the Oxidation of Thiols by Coenzyme PQQ

Kinetic Studies on the Oxidation of Thiols by Coenzyme PQQ

Kinetic studies on the oxidation of thiols by coenzyme PQQ are carried out under anaerobic conditions. A bell shaped pH-rate profile having a maximum rate at around pKa of the thiol is observed. The rate-determining step changes between acidic and basic sides of the profile indicating the existence of at least one intermediate in the course of the reaction.

S. Itoh | M. Mure | Y. Ohshiro | Nobuyuki Kato

[1] S. Itoh,et al. Reaction of Reduced PQQ (PQQH2) and Molecular Oxygen , 1986 .

[2] J. Frank,et al. Covalent addition of H2O, enzyme substrates and activators to pyrrolo-quinoline quinone, the coenzyme of quinoproteins. , 1982, European journal of biochemistry.

[3] J. Frank,et al. Characterization of the second prosthetic group in methanol dehydrogenase from hyphomicrobium X. , 2005, European journal of biochemistry.

[4] E. Loechler,et al. Reduction of flavins by thiols. 1. Reaction mechanism from the kinetics of the attack and breakdown steps , 1980 .

[5] E. Loechler,et al. Reduction of flavins by thiols. 3. The case for concerted N,S-acetal formation in attack and an early transition state in breakdown , 1980 .

[6] T. C. Bruice,et al. Oxidation of thiophenol and nitroalkanes by an electron deficient isoalloxazine , 1975 .

[7] G. Lienhard,et al. Thiol Addition to the Carbonyl Group. Equilibria and Kinetics1 , 1966 .