Tetracycline degradation by ozonation, and evaluation of biodegradability and toxicity of ozonation byproductsPaper submitted to the Journal of Environmental Engineering and Science.

Tetracycline was exposed to ozone by bubbling at different pH levels, and the acute toxicity of ozonation byproducts was tested by luminescent bacteria (Vibrio qinghaiensis sp.-Q67). Residual tetracycline in the solutions was detected by high performance liquid chromatography (HPLC). The results indicated that tetracycline was immediately degraded by ozonation and the degradation was favored at higher pH. Tetracycline (20 mg L–1) was completely degraded after 5 min of ozonation. However, total organic carbon (TOC) hardly decreased even though the ozonation time was extended to 40 min, suggesting that the ozonation process could not mineralize tetracycline. The luminescent bacteria assay indicated that the toxicity of the tetracycline solution was changed after ozonation. Chemical oxygen demand (COD) dropped by 15%, and the BOD5/COD ratio increased from 0.09 to 0.26, suggesting that the biodegradability of tetracycline was enhanced after 20 min of ozonation treatment.

[1]  J. Rivas,et al.  SIMAZINE REMOVAL FROM WATER IN A CONTINUOUS BUBBLE COLUMN BY O3 AND O3/H2O2 , 2001, Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes.

[2]  Christiane Gottschalk,et al.  Ozonation of water and waste water , 2000 .

[3]  R. Augusti,et al.  Monitoring the degradation of tetracycline by ozone in aqueous medium via atmospheric pressure ionization mass spectrometry , 2007, Journal of the American Society for Mass Spectrometry.

[4]  R. Andreozzi,et al.  Lincomycin solar photodegradation, algal toxicity and removal from wastewaters by means of ozonation. , 2006, Water research.

[5]  Jianying Hu,et al.  Antibiotic-resistance profile in environmental bacteria isolated from penicillin production wastewater treatment plant and the receiving river. , 2009, Environmental microbiology.

[6]  Jianying Hu,et al.  EVALUATION OF REACTIVITY OF PESTICIDES WITH OZONE IN WATER USING THE ENERGIES OF FRONTIER , 2000 .

[7]  R Hirsch,et al.  Occurrence of antibiotics in the aquatic environment. , 1999, The Science of the total environment.

[8]  Qiuyun Zhang,et al.  Catalytic ozonation of dimethyl phthalate over cerium supported on activated carbon. , 2009, Journal of Hazardous Materials.

[9]  S. Doğruel,et al.  Combined chemical and biological oxidation of penicillin formulation effluent. , 2004, Journal of environmental management.

[10]  P. Solich,et al.  Tetracycline antibiotics in hospital and municipal wastewaters: a pilot study in Portugal , 2010, Analytical and bioanalytical chemistry.

[11]  Jin-young Jung,et al.  Tetracycline degradation by ozonation in the aqueous phase: proposed degradation intermediates and pathway. , 2010, Journal of hazardous materials.

[12]  G. L. Sant'anna,et al.  Ozonation of azo dyes (Orange II and Acid Red 27) in saline media. , 2009, Journal of hazardous materials.

[13]  Min Yang,et al.  Ozonation of oxytetracycline and toxicological assessment of its oxidation by-products. , 2008, Chemosphere.

[14]  Hans-Peter Bader,et al.  The role of hydroxyl radical reactions in ozonation processes in aqueous solutions , 1976 .

[15]  F. Beltrán,et al.  Ozone and photocatalytic processes to remove the antibiotic sulfamethoxazole from water. , 2008, Water research.

[16]  Cheng-fang Lin,et al.  O(3) and O(3)/H(2)O(2) treatment of sulfonamide and macrolide antibiotics in wastewater. , 2009, Journal of hazardous materials.

[17]  Jun Li,et al.  Occurrence and elimination of antibiotics at four sewage treatment plants in the Pearl River Delta (PRD), South China. , 2007, Water research.

[18]  L. Raskin,et al.  Presence of Macrolide‐Lincosamide‐Streptogramin B and Tetracycline Antimicrobials in Swine Waste Treatment Processes and Amended Soil , 2005, Water environment research : a research publication of the Water Environment Federation.

[19]  Christiane Gottschalk,et al.  Ozonation of Water and Waste Water: A Practical Guide to Understanding Ozone and Its Application , 2000 .

[20]  Cong-Qiang Liu,et al.  [Characteristics of chloramphenicol and tetracyclines in municipal sewage and Nanming River of Guiyang City, China]. , 2009, Huan jing ke xue= Huanjing kexue.

[21]  W. Chu,et al.  Modeling the ozonation of 2,4-dichlorophoxyacetic acid through a kinetic approach. , 2003, Water research.

[22]  Diana S Aga,et al.  Removal of antibiotics in wastewater: Effect of hydraulic and solid retention times on the fate of tetracycline in the activated sludge process. , 2005, Environmental science & technology.

[23]  B. Langlais,et al.  Guideline for Measurement of Ozone Concentration in the Process Gas From an Ozone Generator , 1996 .

[24]  I. Arslan-Alaton,et al.  Ozonation of Procaine Penicillin G formulation effluent Part I: Process optimization and kinetics. , 2005, Chemosphere.

[25]  S. Esplugas,et al.  Sulfamethoxazole abatement by means of ozonation. , 2008, Journal of hazardous materials.

[26]  Johannes Staehelin,et al.  Decomposition of ozone in water: rate of initiation by hydroxide ions and hydrogen peroxide , 1982 .

[27]  Shourong Zheng,et al.  Aqueous photolysis of tetracycline and toxicity of photolytic products to luminescent bacteria. , 2008, Chemosphere.