Excretory/secretory antigen of Toxocara canis: recognition profiles of polyclonal and larvicidal monoclonal antibodies

Summary Five monoclonal antibodies against the excretory/secretory (E/S) antigen of Toxocara canis were obtained and characterized. Immunoprecipitating activity was demonstrated in an in‐vitro micropre‐cipitating assay using live T. canis larvae. Their capacity to kill larvae was also shown in an in‐vitro assay. Two zones of reactivity were observed in 7.5 and 12.5% SDS‐PAGE (177‐77 kD, 43‐15 kD) of immunoprecipitates of human and mouse positive polyclonal anlisera. The murine monoclonal antibodies showed a common pattern of reactivity with the proteins in the 177‐77 kD range.

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