Adhesion of Bacillus spores and Escherichia coli cells to inert surfaces: role of surface hydrophobicity.

The ability of bacterial spores and vegetative cells to adhere to inert surfaces was investigated by means of the number of adherent spores (Bacillus cereus and Bacillus subtilis spores) and Escherichia coli cells and their resistance to cleaning or rinsing procedures (adhesion strength). Six materials (glass, stainless steel, polyethylene high density (PEHD), polyamide-6, polyvinyl chloride, and Teflon) were tested. Slight differences in the number of adherent spores (less than 1 log unit) were observed between materials, but a higher number of adherent E. coli cells was found on the hydrophobic materials PEHD and Teflon. Conversely, the resistance of both B. cereus and B. subtilis spores to a cleaning procedure was significantly affected by the material. Hydrophobic materials were harder to clean. The topography parameter derived from the Abbott-Firestone curve, RVK, and, to a lesser extent, the widely used roughness parameters RA (average roughness) and Rz (maximal roughness), were related to the number of adherent cells. Lastly, the soiling level as well as the adhesion strength were shown to depend largely on the microorganism. The number of adhering B. cereus hydrophobic spores and their resistance to a cleaning procedure were found to be 10 times greater than those of the B. subtilis hydrophilic spores. Escherichia coli was loosely bound to all the materials tested, even after 24 h biofilm formation.

[1]  G. Ripabelli,et al.  Prevalence of Salmonellae, Listeriae, and Yersiniae in the Slaughterhouse Environment and on Work Surfaces, Equipment, and Workers. , 1997, Journal of food protection.

[2]  L. Barnes,et al.  Effect of Milk Proteins on Adhesion of Bacteria to Stainless Steel Surfaces , 1999, Applied and Environmental Microbiology.

[3]  R. Kolter,et al.  Genetic analysis of Escherichia coli biofilm formation: roles of flagella, motility, chemotaxis and type I pili , 1998, Molecular microbiology.

[4]  John D. Brooks,et al.  Properties of the stainless steel substrate, influencing the adhesion of thermo-resistant streptococci , 2000 .

[5]  U. Rönner,et al.  The influence of hydrophobic, electrostatic and morphologic properties on the adhesion of Bacillus spores , 1992 .

[6]  P. Bremer,et al.  The influence of cell surface properties of thermophilic streptococci on attachment to stainlesssteel , 1997, Journal of applied microbiology.

[7]  M. Daeschel,et al.  The adhesion and detachment of bacteria and spores on food-contact surfaces , 1996 .

[8]  A. D. Warth.  Relationship between the heat resistance of spores and the optimum and maximum growth temperatures of Bacillus species , 1978, Journal of bacteriology.

[9]  C. Alexander,et al.  Bacterial Adhesion at Synthetic Surfaces , 1999, Applied and Environmental Microbiology.

[10]  W. Zingg,et al.  Surface thermodynamics of bacterial adhesion , 1983, Applied and environmental microbiology.

[11]  J. Remon,et al.  Kinetics of Pseudomonas aeruginosa adhesion to 304 and 316-L stainless steel: role of cell surface hydrophobicity , 1990, Applied and environmental microbiology.

[12]  J. Holah,et al.  A comparative evaluation with respect to the bacterial cleanability of a range of wall and floor surface materials used in the food industry , 1996 .

[13]  F. Gavini,et al.  Injury and Lethality of Heat Treatment of Bacillus cereus Spores Suspended in Buffer and in Poultry Meat. , 1997, Journal of food protection.

[14]  G. Wirtanen,et al.  Evaluation of cleaning procedures for elimination of biofilm from stainless steel surfaces in open process equipment , 1996 .

[15]  U. Rönner,et al.  Adhesion of Bacillus cereus spores to different solid surfaces: Cleaned or conditioned with various food agents , 1993 .

[16]  M. Chaudhury,et al.  Interfacial Lifshitz-van der Waals and polar interactions in macroscopic systems , 1988 .

[17]  M. Bellon-Fontaine,et al.  The influence of metallic surface wettability on bacterial adhesion , 1993 .

[18]  C. J. Oss,et al.  On the Predominant Electron- Donicity of Polar Solid Surfaces* , 1997 .

[19]  U. Rönner,et al.  Forces involved in adhesion of Bacillus cereus spores to solid surfaces under different environmental conditions. , 1990, The Journal of applied bacteriology.

[20]  M. V. van Loosdrecht,et al.  The role of bacterial cell wall hydrophobicity in adhesion , 1987, Applied and environmental microbiology.

[21]  Å. Henriksson,et al.  Adhesion of bacillus spores in relation to hydrophobicity. , 1990, The Journal of applied bacteriology.

[22]  P. E. Granum,et al.  What problems does the food industry have with the spore-forming pathogens Bacillus cereus and Clostridium perfringens? , 1995, International journal of food microbiology.

[23]  Sharron McEldowney,et al.  Variability of the Influence of Physicochemical Factors Affecting Bacterial Adhesion to Polystyrene Substrata , 1986, Applied and environmental microbiology.

[24]  E. Mettler,et al.  Hygienic Quality of Floors in Relation to Surface Texture , 1999 .

[25]  G. Wirtanen,et al.  Microbial Evaluation of the Biotransfer Potential from Surfaces with Bacillus Biofilms after Rinsing and Cleaning Procedures in Closed Food-Processing Systems. , 1996, Journal of food protection.

[26]  S. Kozuka,et al.  Properties and Origin of Filamentous Appendages on Spores of Bacillus cereus , 1985, Microbiology and immunology.

[27]  J. Carballo,et al.  Attachment of Salmonella spp. and Listeria monocytogenes to stainless steel, rubber and polytetrafluorethylene: the influence of free energy and the effect of commercial sanitizers , 2000 .

[28]  D. Rittschof,et al.  Adhesion and motility of gliding bacteria on substrata with different surface free energies , 1990, Applied and environmental microbiology.

[29]  M. Pierson,et al.  Influence of environmental stress on the kinetics and strength of attachment of Listeria monocytogenes Scott A to Buna-N rubber and stainless steel. , 1998, Journal of food protection.

[30]  T. Benezech,et al.  Influence of physicochemical properties on the hygienic status of stainless steel with various finishes , 2000 .

[31]  T. Koshikawa,et al.  Surface hydrophobicity of spores of Bacillus spp. , 1989, Journal of general microbiology.

[32]  C. J. Oss,et al.  Microbial adhesion to solvents: a novel method to determine the electron-donor/electron-acceptor or Lewis acid-base properties of microbial cells , 1996 .

[33]  H. Busscher,et al.  Deposition of leuconostoc mesenteroides and streptococcus thermophilus to solid substrata in a parallel plate flow cell , 1990 .

[34]  E. Rosenberg,et al.  Bacterial adherence to hydrocarbons and to surfaces in the oral cavity , 1983 .

[35]  M. Bellon-Fontaine,et al.  Adhesion of streptococcus thermophilus to stainless steel with different surface topography and roughness , 1997 .

[36]  Y. Dufrêne,et al.  Adhesion of Azospirillum brasilense: Role of proteins at the cell-support interface , 1996 .

[37]  J. Holah,et al.  Cleanability in relation to bacterial retention on unused and abraded domestic sink materials. , 1990, The Journal of applied bacteriology.

[38]  J. Holah,et al.  The use of epifluorescence microscopy to determine surface hygiene , 1989 .

[39]  S. Kozuka,et al.  Exosporia and Appendages of Spores of Bacillus Species , 1984, Microbiology and immunology.

[40]  T. Benezech,et al.  Potential occurrence of adhering living Bacillus spores in milk product processing lines , 2001, Journal of applied microbiology.

[41]  Rosário Oliveira,et al.  Influence of surface characteristics on the adhesion of Alcaligenes denitrificans to polymeric substrates , 1999 .

[42]  J. Snijders,et al.  Critical points in meat production lines regarding the introduction of Listeria monocytogenes. , 1993, The Veterinary quarterly.