Real-time monitoring of blood using coherent anti-Stokes Raman spectroscopy.

We demonstrate a real-time method of measuring the vibrational Raman spectrum of whole blood. Using a novel coherent Raman technique, we record the vibrational spectrum of the red blood cells from picoliters in blood in milliseconds. This method will allow real-time in vivo blood monitoring.

[1]  W. R. Wiley,et al.  Three-Dimensional Vibrational Imaging by Coherent Anti-Stokes Raman Scattering , 1999 .

[2]  Kevin R Ward,et al.  Oxygenation monitoring of tissue vasculature by resonance Raman spectroscopy. , 2007, Analytical chemistry.

[3]  W. Tolles,et al.  A Review of the Theory and Application of Coherent Anti-Stokes Raman Spectroscopy (CARS) , 1977 .

[4]  Marlan O. Scully,et al.  Real-time detection of bacterial spores using coherent anti-Stokes Raman spectroscopy , 2008 .

[5]  D. Wiersma,et al.  Real-time visualization of intracellular hydrodynamics in single living cells. , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[6]  Thomas G. Spiro,et al.  Biological applications of Raman spectroscopy , 1987 .

[7]  R. W. Terhune,et al.  Study of Optical Effects Due to an Induced Polarization Third Order in the Electric Field Strength , 1965 .

[8]  W. Tolles,et al.  A Comparative Analysis of the Analytical Capabilities of Coherent Anti-Stokes Raman Spectroscopy (CARS) Relative to Raman Scattering and Absorption Spectroscopy , 1977 .

[9]  Yaochun Shen,et al.  Introduction to Nonlinear Laser Spectroscopy , 1984 .

[10]  M. Bonn,et al.  Spectroscopic analysis of the oxygenation state of hemoglobin using coherent anti-Stokes Raman scattering. , 2006, Journal of biomedical optics.

[11]  Marlan O Scully,et al.  Single-shot detection of bacterial endospores via coherent Raman spectroscopy , 2007, Proceedings of the National Academy of Sciences.

[12]  Mischa Bonn,et al.  Direct extraction of Raman line-shapes from congested CARS spectra. , 2006, Optics express.

[13]  Hiro-o Hamaguchi,et al.  Dispersion‐compensated supercontinuum generation for ultrabroadband multiplex coherent anti‐Stokes Raman scattering spectroscopy , 2006 .

[14]  Marlan O Scully,et al.  Optimizing the Laser-Pulse Configuration for Coherent Raman Spectroscopy , 2007, Science.

[15]  Bayden R. Wood,et al.  Raman excitation wavelength investigation of single red blood cells in vivo , 2002 .

[16]  Don McNaughton,et al.  Resonance Raman spectroscopy of red blood cells using near-infrared laser excitation , 2007, Analytical and bioanalytical chemistry.

[17]  Marlan O Scully,et al.  Sensitive femtosecond coherent anti-Stokes Raman spectroscopy discrimination between dipicolinic acid and dinicotinic acid. , 2006, Optics letters.

[18]  M. S. Zubairy,et al.  FAST CARS: Engineering a laser spectroscopic technique for rapid identification of bacterial spores , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[19]  M. Feld,et al.  Blood analysis by Raman spectroscopy. , 2002, Optics letters.

[20]  X. Xie,et al.  Coherent Anti-Stokes Raman Scattering Microscopy: Instrumentation, Theory, and Applications , 2004 .

[21]  B. Prince,et al.  Development of simultaneous frequency- and time-resolved coherent anti-Stokes Raman scattering for ultrafast detection of molecular Raman spectra. , 2006, The Journal of chemical physics.

[22]  M D Duncan,et al.  Scanning coherent anti-Stokes Raman microscope. , 1982, Optics letters.

[23]  S. Kawata,et al.  Molecular vibration imaging in the fingerprint region by use of coherent anti-Stokes Raman scattering microscopy with a collinear configuration. , 2000, Optics letters.

[24]  Erik M. Vartiainen,et al.  Phase retrieval approach for coherent anti-Stokes Raman scattering spectrum analysis , 1992 .