Hypermethylated SUPERMAN Epigenetic Alleles in Arabidopsis

Mutations in the SUPERMAN gene affect flower development in Arabidopsis. Seven heritable but unstable sup epi-alleles (the clark kent alleles) are associated with nearly identical patterns of excess cytosine methylation within the SUP gene and a decreased level of SUP RNA. Revertants of these alleles are largely demethylated at the SUP locus and have restored levels of SUP RNA. A transgenic Arabidopsis line carrying an antisense methyltransferase gene, which shows an overall decrease in genomic cytosine methylation, also contains a hypermethylated sup allele. Thus, disruption of methylation systems may yield more complex outcomes than expected and can result in methylation defects at known genes. The clark kent alleles differ from the antisense line because they do not show a general decrease in genomic methylation.

[1]  J. Ashby.  References and Notes , 1999 .

[2]  FON1, an Arabidopsis gene that terminates floral meristem activity and controls flower organ number. , 1997, The Plant cell.

[3]  T. Kakutani,et al.  Developmental abnormalities and epimutations associated with DNA hypomethylation mutations. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[4]  Reduced DNA methylation in Arabidopsis thaliana results in abnormal plant development. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[5]  S. Dellaporta,et al.  Demethylation-Induced Developmental Pleiotropy in Arabidopsis , 1996, Science.

[6]  Elliot M. Meyerowitz,et al.  Role of SUPERMAN in maintaining Arabidopsis floral whorl boundaries , 1995, Nature.

[7]  R. Martienssen,et al.  DNA methylation in eukaryotes. , 1995, Current opinion in genetics & development.

[8]  J. C. Gaiser,et al.  The Arabidopsis SUPERMAN Gene Mediates Asymmetric Growth of the Outer Integument of Ovules. , 1995, The Plant cell.

[9]  A. Bird,et al.  Methylation analysis on individual chromosomes: improved protocol for bisulphite genomic sequencing. , 1994, Nucleic acids research.

[10]  R. Martienssen,et al.  Arabidopsis thaliana DNA methylation mutants. , 1993, Science.

[11]  D. Weigel,et al.  SUPERMAN, a regulator of floral homeotic genes in Arabidopsis. , 1992, Development.

[12]  F. B. Pickett,et al.  The FLO10 Gene Product Regulates the Expression Domain of Homeotic Genes AP3 and PI in Arabidopsis Flowers. , 1991, The Plant cell.

[13]  J. Bowman,et al.  Early flower development in Arabidopsis. , 1990, The Plant cell.

[14]  E S Lander,et al.  Restriction fragment length polymorphism linkage map for Arabidopsis thaliana. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[15]  M. Koornneef,et al.  EMS- and relation-induced mutation frequencies at individual loci in Arabidopsis thaliana (L.) Heynh , 1982 .