Binding of some primary aromatic amines to certain rat brain particulate fractions.

The unsubstituted aromatic amines β-phenylethylamine and tryptamine, on addition to rat brain homogenates, were found to form relatively stable 'complexes' with certain particulate fractions. Similar particulate 'complexes' were found following intraventricular injections. As a result of this binding the recovery of small quantities of these amines added to brain homogenates was variable and low when compared to other tissue homogenates. Although p-tyramine formed similar 'complexes' both the strength and extent of the binding was much reduced. Subcellular fractionation revealed that β-phenylethylamine, tryptamine, and p-tyramine were associated with a myelin and synaptosomal fraction.