Optoelectronic method for identifying fentanyl and the main metabolite norfentanyl

This article highlights using liquid chromatography and the MRM method [1], the presence of fentanyl and norfentanyl [2] in a biological sample, in this case urine. In the method you can see the presence of each segment in the ion of the substance, obtained by ElectroSpray ionization [1], for which the confirmation is desired. The time of acquisition and the potential collision used to obtain it are specified. The MRM method is performed with a varying concentration gradient of between 10% and 65% [3] over a 9 minute acquisition time at a constant flow rate of 0.3 ml / minute. The gradient increases from 10% to 40% in the first two minutes, then increases from 40% to 65% over the next 3 minutes. The 65% concentration remains for the next 4 minutes.