Identification of an epitope of SARS-coronavirus nucleocapsid protein

ABSTRACTThe nucleocapsid (N) protein of severe acute respiratory syndrome-coronavirus (SARS-CoV) is a major virion structural protein. In this study, two epitopes (N1 and N2) of the N protein of SARS-CoV were predicted by bioinformatics analysis. After immunization with two peptides, the peptides-specific antibodies were isolated from the immunized rabbits. The further experiments demonstrated that N1 peptide-induced polyclonal antibodies had a high affinity to bind to E. coli expressed N protein of SARS-CoV. Furthermore, it was confirmed that N1 peptide-specific IgG antibodies were detectable in the sera of severe acute respiratory syndrome (SARS) patients. The results indicated that an epitope of the N protein has been identified and N protein specific Abs were produced by peptide immunization, which will be useful for the study of SARS-CoV.

[1]  J. Kwang,et al.  Study of protection by recombinant fowl poxvirus expressing C-terminal nucleocapsid protein of infectious bronchitis virus against challenge. , 2001, Avian diseases.

[2]  Christian Drosten,et al.  Characterization of a Novel Coronavirus Associated with Severe Acute Respiratory Syndrome , 2003, Science.

[3]  J. Robins,et al.  Transmission Dynamics and Control of Severe Acute Respiratory Syndrome , 2003, Science.

[4]  Krishna Shankara Narayanan,et al.  Nucleocapsid-Independent Specific Viral RNA Packaging via Viral Envelope Protein and Viral RNA Signal , 2003, Journal of Virology.

[5]  T. Onodera,et al.  DNA mediated immunization with encoding the nucleoprotein gene of porcine transmissible gastroenteritis virus. , 2001, Virus research.

[6]  G. Brooks,et al.  The Coronavirus Infectious Bronchitis Virus Nucleoprotein Localizes to the Nucleolus , 2001, Journal of Virology.

[7]  Yadi Wu,et al.  IFN-γ up-regulation of IL-12 beta 2 receptor is associated with experimental autoimmune uveitis susceptible B10.A mice, but not in EAU resistant BALB/c mice , 2003, Journal of Neuroimmunology.

[8]  Obi L. Griffith,et al.  The Genome Sequence of the SARS-Associated Coronavirus , 2003, Science.

[9]  Michael B Edmond,et al.  Managing SARS amidst uncertainty. , 2003, The New England journal of medicine.

[10]  J. A. Comer,et al.  A novel coronavirus associated with severe acute respiratory syndrome. , 2003, The New England journal of medicine.

[11]  Ezekiel Emanuel.  The Lessons of SARS , 2003, Annals of Internal Medicine.

[12]  L. Enjuanes,et al.  The SARS Coronavirus: A Postgenomic Era , 2003, Science.

[13]  P. Masters,et al.  Genetic Evidence for a Structural Interaction between the Carboxy Termini of the Membrane and Nucleocapsid Proteins of Mouse Hepatitis Virus , 2002, Journal of Virology.

[14]  Yue Sun,et al.  Pertussis Toxin Enhances Th1 Responses by Stimulation of Dendritic Cells 1 , 2003, The Journal of Immunology.

[15]  Yadi Wu,et al.  The suppressive effect of triptolide on experimental autoimmune uveoretinitis by down-regulating Th1-type response. , 2003, International immunopharmacology.