Effect of arildone on modifications of poliovirus in vitro.

Abstract The effect of arildone, a new antiviral agent, on the biological and physical alterations of poliovirus type 2 induced by heat or alkaline treatment in vitro are described. Arildone prevents inactivation of poliovirus infectivity and physical alterations of the virion at 47°. Although arildone protects the biological activity of poliovirus during treatment at pH 10.5, the drug did not fully prevent the physical alterations induced at alkaline pH. Our results indicate differences in the mechanism of destabilization of poliovirus by heat and alkaline treatment in vitro . Furthermore, these results show that arildone interacts directly with the viral capsid. The stabilization of poliovirus in vitro correlates well with prevention of uncoating in the cell by arildone.

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