Evidence that the level of the p55 component of the interleukin (IL) 2 receptor can control IL 2 responsiveness in a murine IL 3‐dependent cell

Although the role of interleukin 2 (IL 2) in mature T lymphocyte function is well documented, its effect on hematopoietic progenitor cells is less well characterized. Here we have used recombinant retroviruses to transduce and express a cDNA clone encoding the p55 component of the human IL 2 receptor (h‐p55), in a murine IL 3‐dependent cell line, BAF3. While the parental cells do not respond to IL 2, the h‐p55‐expressing cells proliferate upon treatment with recombinant IL 2 after an initial lag period. The responsiveness of individual cell clones is correlated with their level of h‐p55 expression, and can be inhibited by Tac monoclonal antibody. Furthermore, growth at limiting IL 2 concentrations selects a subset of cells expressing higher h‐p55 levels from a bulk population. Detailed 125I‐labeled IL 2 binding analysis on the highest h‐p55‐expressing clone detects the presence of 200 high‐affinity (KD = 25 pM) IL 2 receptors. We therefore propose that the level of h‐p55 expression governs the formation of high‐affinity receptors, and hence IL 2 responsiveness in BAF3 cells.

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