Biocompatibility of Gentamicin-coated Hydroxyapatite (Ha) with osteoblast.

This study was carried out to compare the surface morphological changes and cell-to-cell attachment with different concentrations of gentamicin-coated hydroxyapatite (HA) on the osteoblast cell lines. Osteoblast cell lines were cultured and maintained in complete medium, 1:1 HAM’s F12 Medium Dulbecco's modified Eagle's medium without phenol red (DMEM) and incubated at 37oC in 5% CO2. The cell lines were treated with gentamicin-coated HA and undergone the 3-(4,5-di-methylthiazol-2-yl)-2,5- diphenyl tetrazolium bromide (MTT) assay. After 72 hours treatment, the gentamicin-coated HA was processed for viewing the morphological changes by using scanning electron microscopy. The MTT assay results indicated that varying concentrations of gentamicin coated hydroxyapatite do not significantly affect viability of osteoblast. The osteoblast-like cells attached and spread well on the surface of hydroxyapatite and grew into the pores. In conclusion, the gentamicin-coated HA is biocompatible towards osteoblast.

[1]  V. Guarino,et al.  Mineralization behavior with mesenchymal stromal cells in a biomimetic hyaluronic acid-based scaffold. , 2010, Biomaterials.

[2]  R. Weiss,et al.  Hyaluronic Acid Fillers: A Comprehensive Review , 2009, Facial plastic surgery : FPS.

[3]  S. Shimizu,et al.  Peripheral nerve regeneration by the in vitro differentiated-human bone marrow stromal cells with Schwann cell property. , 2007, Biochemical and biophysical research communications.

[4]  P. Clegg,et al.  Biofilms and their relevance to veterinary medicine. , 2007, Veterinary microbiology.

[5]  A. Martini,et al.  Tissue engineering and cartilage regeneration for auricular reconstruction. , 2006, International journal of pediatric otorhinolaryngology.

[6]  P. Gao,et al.  Passage-restricted differentiation potential of mesenchymal stem cells into cardiomyocyte-like cells. , 2005, Biochemical and biophysical research communications.

[7]  G. Daculsi,et al.  In Vitro and In Vivo Bactericidal Activities of Vancomycin Dispersed in Porous Biodegradable Poly(ε-Caprolactone) Microparticles , 2005, Antimicrobial Agents and Chemotherapy.

[8]  Xiran Zhang,et al.  Mesenchymal Stern Cells from Adult Human Bone Marrow Differentiate into a Cardiomyocyte Phenotype In Vitro , 2004, Experimental biology and medicine.

[9]  E. Drenkard.  Antimicrobial resistance of Pseudomonas aeruginosa biofilms. , 2003, Microbes and infection.

[10]  Weixin Liu,et al.  Growth and differentiation of rat bone marrow stromal cells: does 5-azacytidine trigger their cardiomyogenic differentiation? , 2003, Cardiovascular research.

[11]  E. Sim,et al.  Transformation of adult mesenchymal stem cells isolated from the fatty tissue into cardiomyocytes. , 2003, The Annals of thoracic surgery.

[12]  E. Caterson,et al.  Human marrow-derived mesenchymal progenitor cells , 2002, Molecular biotechnology.

[13]  E. Horwitz,et al.  Bone marrow mesenchymal cells: biological properties and clinical applications. , 2001, Journal of biological regulators and homeostatic agents.

[14]  B Brent,et al.  Technical advances in ear reconstruction with autogenous rib cartilage grafts: personal experience with 1200 cases. , 1999, Plastic and reconstructive surgery.

[15]  A. Caplan.  Mesenchymal stem cells , 1991, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[16]  D. Seligson,et al.  The prophylactic use of antibiotic impregnated beads in open fractures. , 1990, The Journal of trauma.