Immune responses and resistance to brucellosis in mice vaccinated orally with Brucella abortus RB51

Immune responses and resistance to infection with Brucella abortus 2308 (S2308) were measured in mice following oral or intraperitoneal (i.p.) vaccination with strain RB51 (SRB51). Bacteria persisted in the parotid lymph node for 4 weeks following oral vaccination of mice with 5 x 10(8) or 5 x 10(6) CFU of SRB51. Bacteria did not appear in the spleen during 12 weeks after oral vaccination, whereas they did appear in the spleen for 8 weeks following i.p. vaccination of mice with SRB51 (5 x 10(8) or 5 x 10(6) CFU). Increased resistance to S2308 infection occurred at 12 to 20 weeks in mice vaccinated i.p. with SRB51 (5 x 10(8) or 5 x 10(6) CFU) but occurred at 12 weeks only in mice vaccinated orally with SRB51 (5 x 10(8) CFU). Oral SRB51 vaccination induced lower levels of antibodies to the surface antigens of intact SRB51 bacteria than did i.p. vaccination. However, neither route of vaccination induced anamnestic antibody responses to the surface antigens of intact S2308 bacteria after challenge infection of the vaccinated mice with S2308. Mice vaccinated orally with SRB51 and challenged with S2308 at 12 to 20 weeks had lower and less persistent spleen cell proliferation and production of gamma interferon in response to S2308 and certain immunodominant S2308 proteins (32 to < or = 18 kDa) than did mice vaccinated i.p. with SRB51. However, mice vaccinated orally or i.p. with SRB51 and challenged with S2308 had similar spleen cell tumor necrosis factor alpha production. These results indicate that oral vaccination of mice with SRB51 was effective in inducing protective immunity to S2308 infection, although the immunity was lower and less persistent than that induced by i.p. vaccination. The lower protective immunity induced by oral vaccination may have resulted from lower and less persistent cell-mediated immunity and gamma interferon production in response to S2308 and S2308 proteins.

[1]  S. Elberg,et al.  Immunization against Brucella infections: immune response of mice, guinea pigs, and Cynomolgus philipinensis to live and killed Brucella melitensis strain Rev. I administered by various methods. , 1970, The Journal of infectious diseases.

[2]  P. Kennedy,et al.  Pathology of domestic animals , 1970 .

[3]  K. Burchard The hematopoietic system , 2012 .

[4]  P. Pardon,et al.  Resistance of Brucella abortus infected mice to intravenous or intraperitoneal Brucella reinfection. , 1976, Annales d'immunologie.

[5]  E. T. Thorne,et al.  BRUCELLOSIS IN ELK I. SEROLOGIC AND BACTERIOLOGIC SURVEY IN WYOMING1 , 1978, Journal of wildlife diseases.

[6]  N. Bosseray Mother to young transmission of Brucella abortus infection in mouse model. , 1982, Annales de recherches veterinaires. Annals of veterinary research.

[7]  F. Milward,et al.  Protection by oral administration of brucella abortus strain 19 against an oral challenge exposure with a pathogenic strain of Brucella. , 1983, American journal of veterinary research.

[8]  N. Bosseray,et al.  Theoretical, practical and statistical basis for a general control method of activity for anti-Brucella vaccines. , 1984, Developments in biological standardization.

[9]  X. Xin Orally administrable brucellosis vaccine: Brucella suis strain 2 vaccine. , 1986, Vaccine.

[10]  T. Ficht,et al.  BRUCELLA ABORTUS IN CAPTIVE BISON. I. SEROLOGY, BACTERIOLOGY, PATHOGENESIS, AND TRANSMISSION TO CATTLE , 1990, Journal of wildlife diseases.

[11]  G. Schurig,et al.  Experimental infection of goat fetuses in utero with a stable, rough mutant of Brucella abortus. , 1991, Research in veterinary science.

[12]  G. Schurig,et al.  Biological properties of RB51; a stable rough strain of Brucella abortus. , 1991, Veterinary microbiology.

[13]  L. Tabatabai,et al.  Establishment of dose-response relationships in BALB/c mice, using Brucella cell surface protein and lipopolysaccharide. , 1991, American journal of veterinary research.

[14]  G. Schurig,et al.  Bacterial survival, lymph node changes, and immunologic responses of cattle vaccinated with standard and mutant strains of Brucella abortus. , 1992, American journal of veterinary research.

[15]  L. Tabatabai,et al.  Effects of gamma interferon and indomethacin in preventing Brucella abortus infections in mice , 1992, Infection and immunity.

[16]  C. Cheers,et al.  Endogenous gamma interferon mediates resistance to Brucella abortus infection , 1993, Infection and immunity.

[17]  V. Valli,et al.  CHAPTER 2 – The Hematopoietic System , 1993 .

[18]  C. Cheers,et al.  Cytokine response of T-cell subsets from Brucella abortus-infected mice to soluble Brucella proteins , 1993, Infection and immunity.

[19]  A. Kelso,et al.  Cytokine production in the murine response to brucella infection or immunization with antigenic extracts. , 1993, Immunology.

[20]  S. Halling,et al.  Immune responses and protection against infection and abortion in cattle experimentally vaccinated with mutant strains of Brucella abortus. , 1993, American journal of veterinary research.

[21]  X. Jiang,et al.  Effects of cytokines on intracellular growth of Brucella abortus , 1993, Infection and immunity.

[22]  X. Jiang,et al.  Iron augments macrophage-mediated killing of Brucella abortus alone and in conjunction with interferon-gamma. , 1993, Cellular immunology.

[23]  E. Williams,et al.  Brucellosis in Free-ranging Bison (Bison bison) from Teton County, Wyoming , 1993, Journal of wildlife diseases.

[24]  M. Palmer,et al.  Immune and pathologic responses in mice infected with Brucella abortus 19, RB51, or 2308 , 1994, Infection and immunity.

[25]  S. Olsen,et al.  Lymphocyte proliferation in response to immunodominant antigens of Brucella abortus 2308 and RB51 in strain 2308-infected cattle , 1994, Infection and immunity.

[26]  S. Olsen,et al.  Serologic responses in diagnostic tests for brucellosis in cattle vaccinated with Brucella abortus 19 or RB51 , 1994, Journal of clinical microbiology.

[27]  A. Winter,et al.  Macrophages and Brucella. , 1994, Immunology series.

[28]  L. Tabatabai,et al.  Alteration of protective and serologic responses in BALB/c mice vaccinated with chemically modified versus nonmodified proteins of Brucella abortus 19 , 1994, Infection and immunity.

[29]  W. J. Quinn,et al.  Abortion Caused by Brucella abortus Biovar 1 in a Free-ranging Bison (Bison bison) from Yellowstone National Park , 1994, Journal of wildlife diseases.

[30]  J M Blasco,et al.  Vaccination with Brucella abortus rough mutant RB51 protects BALB/c mice against virulent strains of Brucella abortus, Brucella melitensis, and Brucella ovis , 1994, Infection and immunity.

[31]  C. Cheers,et al.  Differential induction of macrophage-derived cytokines by live and dead intracellular bacteria in vitro , 1995, Infection and immunity.

[32]  S. Olsen,et al.  Comparative analysis of immune responses in cattle vaccinated with Brucella abortus strain 19 or strain RB51. , 1995, Veterinary immunology and immunopathology.

[33]  M. G. Stevens,et al.  Effect of Brucella abortus transfer factor in preventing murine brucellosis. , 1995, FEMS immunology and medical microbiology.

[34]  S. Olsen,et al.  Comparison of immune responses and resistance to brucellosis in mice vaccinated with Brucella abortus 19 or RB51 , 1995, Infection and immunity.

[35]  S. Olsen,et al.  Comparison of spleen cell proliferation in response to Brucella abortus 2308 lipopolysaccharide or proteins in mice vaccinated with strain 19 or RB51 , 1995, Infection and immunity.

[36]  S. Olsen,et al.  Lymphocyte proliferation in response to Brucella abortus RB51 and 2308 proteins in RB51-vaccinated or 2308-infected cattle , 1996, Infection and immunity.

[37]  M. Palmer,et al.  Effects of age at vaccination on efficacy of Brucella abortus strain RB51 to protect cattle against brucellosis. , 1996, American journal of veterinary research.

[38]  L. Tabatabai,et al.  Variation of Brucella abortus 2308 infection in BALB/c mice induced by prior vaccination with salt-extractable periplasmic proteins from Brucella abortus 19 , 1996, Infection and immunity.

[39]  S. Olsen,et al.  Antibody responses to Brucella abortus 2308 in cattle vaccinated with B. abortus RB51 , 1996, Infection and immunity.