Composite ECM-alginate microfibers produced by microfluidics as scaffolds with biomineralization potential.

A novel approach to produce artificial bone composites (microfibers) with distinctive features mimicking natural tissue was investigated. Currently proposed inorganic materials (e.g. apatite matrixes) lack self-assembly and thereby limit interactions between cells and the material. The present work investigates the feasibility of creating "bio-inspired materials" specifically designed to overcome certain limitations inherent to current biomaterials. We examined the dimensions, morphology, and constitutive features of a composite hydrogel which combined an alginate based microfiber with a gelatin solution or a particulate form of urinary bladder matrix (UBM). The effectiveness of the composite microfibers to induce and modulate osteoblastic differentiation in three-dimensional (3D) scaffolds without altering the viability and morphological characteristics of the cells was investigated. The present study describes a novel alginate microfiber production method with the use of microfluidics. The microfluidic procedure allowed for precise tuning of microfibers which resulted in enhanced viability and function of embedded cells.

[1]  David J Mooney,et al.  Coating of VEGF-releasing scaffolds with bioactive glass for angiogenesis and bone regeneration. , 2006, Biomaterials.

[2]  M. Pokrywczyńska,et al.  Alginate is not a good material for growth of rapidly proliferating cells. , 2008, Transplantation proceedings.

[3]  R. Gambari,et al.  Correlation between Slug transcription factor and miR-221 in MDA-MB-231 breast cancer cells , 2012, BMC Cancer.

[4]  Jae-Do Nam,et al.  Electrospun dual-porosity structure and biodegradation morphology of Montmorillonite reinforced PLLA nanocomposite scaffolds. , 2005, Biomaterials.

[5]  Jon A. Rowley,et al.  Controlling Mechanical and Swelling Properties of Alginate Hydrogels Independently by Cross-Linker Type and Cross-Linking Density , 2000 .

[6]  Y. Ikada,et al.  Histologic and mechanical evaluation for bone bonding of polymer surfaces grafted with a phosphate-containing polymer. , 1997, Journal of biomedical materials research.

[7]  M. Axelos,et al.  Physico-chemical properties and rheology of alginate gel beads formed with various divalent cations , 1998 .

[8]  V. Y. Ng,et al.  Risk of disease transmission with bone allograft. , 2012, Orthopedics.

[9]  D. Kaplan,et al.  Porosity of 3D biomaterial scaffolds and osteogenesis. , 2005, Biomaterials.

[10]  A. Malkin,et al.  Barus effect in polymer flows through cylindrical and flat dies , 1976 .

[11]  F H Silver,et al.  Collagen fibrillogenesis in vitro: comparison of types I, II, and III. , 1984, Archives of biochemistry and biophysics.

[12]  A. Meunier,et al.  Tissue-engineered bone regeneration , 2000, Nature Biotechnology.

[13]  F. Volke,et al.  Towards a medically approved technology for alginate-based microcapsules allowing long-term immunoisolated transplantation , 2005, Journal of materials science. Materials in medicine.

[14]  P. de Vos,et al.  Alginate-based microcapsules for immunoisolation of pancreatic islets. , 2006, Biomaterials.

[15]  Amy L. Ladd,et al.  Bone graft substitutes , 2002 .

[16]  Antonios G Mikos,et al.  Biomimetic materials for tissue engineering. , 2003, Biomaterials.

[17]  Y. Gong,et al.  A study on the bioactivity of chitosan/nano-hydroxyapatite composite scaffolds for bone tissue engineering , 2006 .

[18]  M Falconi,et al.  Preparation and validation of low cost microfluidic chips using a shrinking approach. , 2014, Lab on a chip.

[19]  P. Ma,et al.  Ionically crosslinked alginate hydrogels as scaffolds for tissue engineering: part 1. Structure, gelation rate and mechanical properties. , 2001, Biomaterials.

[20]  S. Dorozhkin,et al.  Nanosized and nanocrystalline calcium orthophosphates. , 2010, Acta biomaterialia.

[21]  David Cheneler,et al.  Mechanical properties of alginate hydrogels manufactured using external gelation. , 2014, Journal of the mechanical behavior of biomedical materials.

[22]  Melba Navarro,et al.  New macroporous calcium phosphate glass ceramic for guided bone regeneration. , 2004, Biomaterials.

[23]  Mark L. Benson,et al.  Isolation of vascular smooth muscle cells from a single murine aorta. , 2001, Methods in cell science : an official journal of the Society for In Vitro Biology.

[24]  D. Hutmacher,et al.  Scaffolds in tissue engineering bone and cartilage. , 2000, Biomaterials.

[25]  N. Chronis,et al.  A Biochip with a 3D microfluidic architecture for trapping white blood cells. , 2013, Sensors and actuators. B, Chemical.

[26]  G. Whitesides.  The origins and the future of microfluidics , 2006, Nature.

[27]  D. Beruto,et al.  A bone substitute composed of polymethylmethacrylate and alpha-tricalcium phosphate: results in terms of osteoblast function and bone tissue formation. , 2002, Biomaterials.

[28]  Shozo Takagi,et al.  Synergistic reinforcement of in situ hardening calcium phosphate composite scaffold for bone tissue engineering. , 2004, Biomaterials.

[29]  K. Leong,et al.  Solid freeform fabrication of three-dimensional scaffolds for engineering replacement tissues and organs. , 2003, Biomaterials.

[30]  Eleftherios Tsiridis,et al.  Current concepts of molecular aspects of bone healing. , 2005, Injury.

[31]  Hongkai Wu,et al.  Generation of alginate microfibers with a roller-assisted microfluidic system. , 2009, Lab on a chip.

[32]  Richard O C Oreffo,et al.  Bridging the regeneration gap: stem cells, biomaterials and clinical translation in bone tissue engineering. , 2008, Archives of biochemistry and biophysics.

[33]  M. Viana,et al.  Fabrication of porous substrates: a review of processes using pore forming agents in the biomaterial field. , 2008, Journal of pharmaceutical sciences.