论文信息 - Automation of bone marrow aspirate examination using the XE‐2100 automated hematology analyzer

Automation of bone marrow aspirate examination using the XE‐2100 automated hematology analyzer

Attempts to analyze bone marrow aspirates have been reported with the use of several automated blood cell counters, but sufficient accuracy in examination is not acquired yet. Major problems have included difficulties in correctly differentiating various immature cells and interference by lipid in bone marrow aspirates. The goal of this study was to solve these problems to attain more accurate assessment of bone marrow aspirates with automated blood cell counters.

[1] S. Ichiyama,et al. Flow cytometric method for enumeration and classification of reactive immature granulocyte populations. , 2000, Cytometry.

[2] K. Ohta,et al. Automated Enumeration of Cellular Composition in Bone Marrow Aspirate with the CELL-DYN 4000TM Automated Hematology Analyzer , 1999, Acta Haematologica.

[3] B. Houwen,et al. New rapid flow cytometric method for the enumeration of nucleated red blood cells. , 1999, Cytometry.

[4] M. Loken,et al. CD45 Gating for Routine Flow Cytometric Analysis of Human Bone Marrow Specimens , 1993, Annals of the New York Academy of Sciences.

[5] S. Bentley,et al. Correction of bone marrow nucleated cell counts for the presence of fat particles. , 1995, American journal of clinical pathology.

[6] L. Laurenti,et al. Automated Analysis of Bone Marrow Fluid with the Sysmex XE-2100TM Blood Cell Counter , 2001, Transfusion Medicine and Hemotherapy.

[7] M. Borowitz,et al. Immunophenotyping of acute leukemia by flow cytometric analysis. Use of CD45 and right-angle light scatter to gate on leukemic blasts in three-color analysis. , 1993, American journal of clinical pathology.

[8] N. Tatsumi,et al. Counting and differential of bone marrow cells by an electronic method. , 1986, American journal of clinical pathology.