Study of Phytoconstituents and antibacterial activity of Kappaphycus alvarezii

The aim of this investigation was to analyse the antimicrobial activity of Kappaphycus alvarezii and to study its bioactive compounds. The crude extracts of Kappaphycus alvarezii extracted in ethanol and chloroform was subjected to preliminary phytochemical estimation. Its activity against gram positive and negative bacterial strains of Escherichia coli, Staphylococcus aureus, Proteus mirabilis, Streptococcus pneumoniae, Pseudomonas aeruginosa, Vibrio cholerae, and Bacillus subtilis was also investigated. The compounds present were confirmed using HPLC and the functional groups were identified using FT-IR. Phytochemical analysis tested positive for the presence of flavonoids, cardiac glycosides, sterols and quinones. FTIR analysis of the EtOH and CHCl3 extracts of Kappaphycus alvarezii showed similar peaks corresponding to functional groups such as amines (-NH2), alcohols (-OH) and carboxyl (-C=O) groups. The qualitative HPLC fingerprint profile of both extracts showed prospective peaks at lower Rf values indicating the major presence of quinones.The results show that, the presence of bioactive quinones, sterols and isoflavonols, lead to active inhibition of microbial growth in a dose-dependent manner. K e y w o r d s Antibacterial Activity, bioactive , quinones, sterols and isoflavonols

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