Surfactants identified in synovial fluid and their ability to act as boundary lubricants.

Thin-layer chromatography has been used to identify phospholipids extracted from canine synovial fluid, the major component (45%) being phosphatidyl choline (PC). The extracts and their components have been shown to be surface active in reducing the surface tension of water and to be readily adsorbed to hydrophilic solids, whose surfaces then become hydrophobic. These adsorbed monolayers of synovial surfactant were then found to be excellent boundary lubricants in vitro, reducing the coefficient of kinetic friction (mu) in the dry state and under physiological loading by up to 97% for extracts and 99% for PC alone, reaching mu = 0.01. Surface-active phospholipid is put forward as the possible active ingredient in joint lubrication and shown to be consistent with previous biochemical studies to elucidate its identity. The model essentially follows the classical Hardy model for boundary lubrication imparted by surfactants. It is discussed in relation to a new approach in providing artificial lubrication and facilitating tissue release in patients with arthritis.

[1]  B. Hills Contact-angle hysteresis induced by pulmonary surfactants. , 1983, Journal of applied physiology: respiratory, environmental and exercise physiology.

[2]  K. B. Blodgett Films Built by Depositing Successive Monomolecular Layers on a Solid Surface , 1935 .

[3]  C W McCutchen Joint lubrication. , 1983, Bulletin of the Hospital for Joint Diseases Orthopaedic Institute.

[4]  J. Folch,et al.  Preparation of lipide extracts from brain tissue. , 1951, The Journal of biological chemistry.

[5]  L. Sokoloff The Biology of Degenerative Joint Disease , 2015, Perspectives in biology and medicine.

[6]  F. C. Linn Lubrication of Animal Joints , 1969 .

[7]  E. Bywaters The Joints and Synovial Fluid , 1981 .

[8]  J. Faber,et al.  Lubrication of joints. , 1967, Journal of applied physiology.

[9]  I. Paul,et al.  Response of joints to impact loading. 3. Relationship between trabecular microfractures and cartilage degeneration. , 1973, Journal of biomechanics.

[10]  E. Radin,et al.  The lubricating activity of synovial fluid glycoproteins. , 1981, Arthritis and rheumatism.

[11]  E. Radin,et al.  Separation of a Hyaluronate-free Lubricating Fraction from Synovial Fluid , 1970, Nature.

[12]  B. Butler,et al.  Gastric mucosal barrier: hydrophobic lining to the lumen of the stomach. , 1983, The American journal of physiology.

[13]  B. Butler,et al.  Boundary lubrication imparted by pleural surfactants and their identification. , 1982, Journal of applied physiology: respiratory, environmental and exercise physiology.

[14]  D. Shah,et al.  BINDING OF METAL IONS TO MONOLAYERS OF LECITHINS, PLASMALOGEN, CARDIOLIPIN, AND DICETYL PHOSPHATE. , 1965, Journal of lipid research.

[15]  E. R. Booser,et al.  Standard Handbook of Lubrication Engineering , 1968 .

[16]  C. McCutchen Lubrication of Joints , 1964 .

[17]  S. Yariv,et al.  Physical Chemistry of Surfaces , 1979 .

[18]  L. van Deenen,et al.  Molecular species of lecithins from various animal tissues. , 1971, Biochimica et biophysica acta.

[19]  J. Folch,et al.  A simple method for the isolation and purification of total lipides from animal tissues. , 1957, The Journal of biological chemistry.

[20]  G. Gaines,et al.  Insoluble Monolayers at Liquid-gas Interfaces , 1966 .

[21]  D Dowson,et al.  Lubrication of synovial membrane. , 1976, Annals of the rheumatic diseases.

[22]  J. D. Reid,et al.  Studies on the Partial Carboxymethylation of Cotton , 1952 .

[23]  Mcutchen Cw Boundary lubrication by synovial fluid: demonstration and possible osmotic explanation. , 1966 .

[24]  D. Mcclean Studies on diffusing factors: 2. Methods of assay of hyaluronidase and their correlation with skin diffusing activity. , 1943, The Biochemical journal.

[25]  F. C. Linn,et al.  Lubrication of animal joints. 3. The effect of certain chemical alterations of the cartilage and lubricant. , 1968, Arthritis and rheumatism.

[26]  N. Gershfeld Selective phospholipid adsorption and atherosclerosis. , 1979, Science.

[27]  M. Frosolono,et al.  Isolation, characterization, and surface chemistry of a surface-active fraction from dog lung. , 1970, Journal of lipid research.

[28]  M. Macconaill The Function of Intra-Articular Fibrocartilages, with Special Reference to the Knee and Inferior Radio-Ulnar Joints. , 2022, Journal of anatomy.

[29]  EricShirley Jones,et al.  NOTES, COMMENTS, AND ABSTRACTSJOINT LUBRICATION , 1934 .

[30]  B. Hills Analysis of eustachian surfactant and its function as a release agent. , 1984, Archives of otolaryngology.

[31]  Macconaill Ma The Function of Intra-Articular Fibrocartilages, with Special Reference to the Knee and Inferior Radio-Ulnar Joints , 1932 .

[32]  G. Higginson,et al.  The Lubrication of Porous Elastic Solids with Reference to the Functioning of Human Joints , 1974 .

[33]  I. Paul,et al.  Response of joints to impact loading. I. In vitro wear. , 1971, Arthritis and rheumatism.

[34]  S. Tolksdorf,et al.  The turbidimetric assay of hyaluronidase. , 1949, The Journal of laboratory and clinical medicine.

[35]  C. McCutchen,et al.  Mechanism of Animal Joints: Sponge-hydrostatic and Weeping Bearings , 1959, Nature.

[36]  W. D. Harkins,et al.  I. A Simple Accurate Film Balance of the Vertical Type for Biological and Chemical Work, and a Theoretical and Experimental Comparison with the Horizontal Type. II. Tight Packing of a Monolayer by Ions , 1937 .

[37]  F. C. Linn,et al.  Lubrication of animal joints. II. The mechanism. , 1968, Journal of biomechanics.

[38]  B. Hills Water repellency induced by pulmonary surfactants. , 1982, The Journal of physiology.

[39]  C. W. McCutchen,et al.  Lubrication of and by Articular Cartilage , 1983 .

[40]  C. Curtain The nature of the protein in the hyaluronic complex of bovine synovial fluid. , 1955, Biochemical Journal.

[41]  S. Fleischer,et al.  Quantitative analysis of phospholipids by thin-layer chromatography and phosphorus analysis of spots , 2007, Lipids.

[42]  B. Hills What is the true role of surfactant in the lung? , 1981, Thorax.

[43]  C. Meban An electron microscopic study of the acid mucosubstance lining the alveoli of hamster lung , 2005, The Histochemical Journal.

[44]  V. P. Skipski,et al.  THIN-LAYER CHROMATOGRAPHY OF PHOSPHOLIPIDS USING SILICA GEL WITHOUT CALCIUM SULFATE BINDER. , 1963, Journal of lipid research.

[45]  Hugh Davson,et al.  A Textbook of General Physiology , 1952, The Yale Journal of Biology and Medicine.