CD271 Negative Human Dental Pulp Cells Yield Significantly More Adherent Colony Forming Cells than the Positive Phenotype

Cell surface markers for isolating proliferative human dental pulp stromal cells are currently lacking. Other tissues containing mesenchymal stromal cells have been studied in greater depth and candidate markers for cell isolation identified, one such marker being CD271. Previous reports suggest CD271 as a marker for isolating dental pulp stromal cells from rat incisors. We aimed to study the utility of CD271 as a marker for isolating human dental pulp stromal cells. CD271 positive cells from both third molar dental pulp and bone marrow mononuclear cells were isolated by magnetic separation followed by in vitro expansion. Phenotypic analysis was performed by flow cytometry. Our data showed that although CD271 is present in the adult molar dental pulp, significantly greater numbers of colonies are produced from the CD271 negative fraction. The opposite was seen with bone marrow mononuclear cells; all colony forming cells were derived from the CD271 positive fraction. Phenotypic analysis of expanded CD271 negative cells showed that these cells are identical to dental pulp stromal cells isolated by non-selective plastic adherence. We conclude that, in contrast to bone marrow, CD271 is not a positive selection marker for the predominant colony forming cell types from human molar dental pulp.

[1]  S. De Biasi,et al.  Human Dental pulp stem cells (hDPSCs): isolation, enrichment and comparative differentiation of two sub-populations , 2015, BMC Developmental Biology.

[2]  Xuebin B. Yang,et al.  Tissue non-specific alkaline phosphatase production by human dental pulp stromal cells is enhanced by high density cell culture , 2015, Cell and Tissue Research.

[3]  Li Xiao,et al.  Characterization of human dental pulp cells‐derived spheroids in serum‐free medium: Stem cells in the core , 2013, Journal of cellular biochemistry.

[4]  Xuebin B. Yang,et al.  Mesenchymal Stem Cells and Skeletal Regeneration , 2013 .

[5]  N. Takakura,et al.  Origins and Properties of Dental, Thymic, and Bone Marrow Mesenchymal Cells and Their Stem Cells , 2012, PLoS ONE.

[6]  M. Miura,et al.  Neural crest stem cell property of apical pulp cells derived from human developing tooth , 2012, Cell biology international.

[7]  E. Wolfs,et al.  Expression Pattern of Basal Markers in Human Dental Pulp Stem Cells and Tissue , 2012, Cells Tissues Organs.

[8]  P. Giannoudis,et al.  High abundance of CD271+ multipotential stromal cells (MSCs) in intramedullary cavities of long bones , 2012, Bone.

[9]  M. Ehinger,et al.  CD146 expression on primary nonhematopoietic bone marrow stem cells is correlated with in situ localization. , 2011, Blood.

[10]  Y. Ishii,et al.  CD271/p75(NTR) inhibits the differentiation of mesenchymal stem cells into osteogenic, adipogenic, chondrogenic, and myogenic lineages. , 2011, Stem cells and development.

[11]  G. Pirozzi,et al.  Ecto-mesenchymal stem cells from dental pulp are committed to differentiate into active melanocytes. , 2010, European cells & materials.

[12]  R. Tuan,et al.  Stem/progenitor cells from inflamed human dental pulp retain tissue regeneration potential. , 2010, Regenerative medicine.

[13]  P. Emery,et al.  Large-scale extraction and characterization of CD271+ multipotential stromal cells from trabecular bone in health and osteoarthritis: implications for bone regeneration strategies based on uncultured or minimally cultured multipotential stromal cells. , 2010, Arthritis and rheumatism.

[14]  Yuk-Kwan Chen,et al.  Isolation and characterization of human dental pulp stem/stromal cells from nonextracted crown-fractured teeth requiring root canal therapy. , 2009, Journal of endodontics.

[15]  L. Kanz,et al.  Isolation of functionally distinct mesenchymal stem cell subsets using antibodies against CD56, CD271, and mesenchymal stem cell antigen-1 , 2009, Haematologica.

[16]  S. Badylak,et al.  A perivascular origin for mesenchymal stem cells in multiple human organs. , 2008, Cell stem cell.

[17]  R. Waddington,et al.  Isolation of Distinct Progenitor Stem Cell Populations from Dental Pulp , 2008, Cells Tissues Organs.

[18]  R. Kelsh,et al.  The emergence of ectomesenchyme , 2008, Developmental dynamics : an official publication of the American Association of Anatomists.

[19]  W. Cui,et al.  Stem cell differentiation and expansion for clinical applications of tissue engineering , 2007, Journal of cellular and molecular medicine.

[20]  I. Kerkis,et al.  Isolation and Characterization of a Population of Immature Dental Pulp Stem Cells Expressing OCT-4 and Other Embryonic Stem Cell Markers , 2007, Cells Tissues Organs.

[21]  J. Jansen,et al.  Multilineage differentiation potential of stem cells derived from human dental pulp after cryopreservation. , 2006, Tissue engineering.

[22]  Paul Emery,et al.  Isolation and characterization of bone marrow multipotential mesenchymal progenitor cells. , 2002, Arthritis and rheumatism.

[23]  A. Boyde,et al.  Stem Cell Properties of Human Dental Pulp Stem Cells , 2002, Journal of dental research.

[24]  D. Soligo,et al.  Isolation of bone marrow mesenchymal stem cells by anti-nerve growth factor receptor antibodies. , 2002, Experimental hematology.

[25]  P. Krebsbach,et al.  Dental and skeletal stem cells: potential cellular therapeutics for craniofacial regeneration. , 2002, Journal of dental education.

[26]  B. Hansen,et al.  Spatiotemporal expression of NGFR during pre-natal human tooth development. , 2002, Orthodontics & craniofacial research.

[27]  S. Gronthos,et al.  Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. , 2000, Proceedings of the National Academy of Sciences of the United States of America.

[28]  E A Morris,et al.  Isolation, characterization, and chondrogenic potential of human bone marrow‐derived multipotential stromal cells , 2000, Journal of cellular physiology.

[29]  A. McMahon,et al.  Fate of the mammalian cranial neural crest during tooth and mandibular morphogenesis. , 2000, Development.

[30]  T. Okiji,et al.  Co-increase of Nerve Fibers and HLA-DRand/or Factor-XIIIa-expressing Dendritic Cells in Dentinal Caries-affected Regions of the Human Dental Pulp: An Immunohistochemical Study , 1999, Journal of dental research.

[31]  P. Couble,et al.  Patterns of nerve growth factor (NGF), proNGF, and p75 NGF receptor expression in the rat incisor: comparison with expression in the molar. , 1993, Differentiation; research in biological diversity.

[32]  M. Bothwell,et al.  Multiple functions for NGF receptor in developing, aging and injured rat teeth are suggested by epithelial, mesenchymal and neural immunoreactivity. , 1990, Development.

[33]  E. Diamanti,et al.  Dental Pulp cells enhance bone healing in a rat osteotomy model , 2014 .

[34]  A. Ho,et al.  Heterogeneity of mesenchymal stromal cell preparations. , 2008, Cytotherapy.

[35]  D. Prockop,et al.  Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. , 2006, Cytotherapy.

[36]  T. Iwanaga,et al.  Immunocytochemical localization of nerve growth factor receptor (NGFR) in human teeth. , 1992, Proceedings of the Finnish Dental Society. Suomen Hammaslaakariseuran toimituksia.