Penja fish is a group of Gobioidei fish and belongs to amphidromous migratory fish, being present throughout the waters that have access to the river as a route for its migration. One location that is currently still inhabited by this fish group is Leppangan River, West Sulawesi. This study was aimed to determine the species of penja fish (amphidromous goby) within the Leppangan river. Fish samples were collected using trap net and clove oil, further identified based on their morphometric and meristic characteristics. The number of penja fish caught in the Leppangan River was 174 individuals consisting of 6 genera and 9 species. The results of identification of all samples consisted of Sicyopterus lagocephalus, Sicyopterus longifilis, Stiphodon semoni, Stiphodon atropurpureus, Sicyopus zosterophorum, Smilosicyopus leprurus, Schismatogobius sp, Eleotris fusca and Eleotris sp. Species of S. zosterophorum has the largest number of 67 individuals and dominated about 38.5% of the total number of species caught in the Leppangan river during the study. The presence of penja fish in the Leppangan river adds information on the distribution of Gobioidei fish populations, particularly those which migrate amphidromously.
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