Effects of methionine on selenium embryotoxicity in cultured rat embryos.

Effects of methionine, an essential amino acid, on the embryotoxicity of selenium (Se) were examined using the rat embryo culture. Rat embryos at day 9.5 of gestation were cultured for 48 h in the presence of sodium selenite at 10 and 20 microM or sodium selenate at 30 and 100 microM with or without the addition of 1 mM DL-methionine. Selenite at 20 microM or selenate at 100 microM alone increased the incidence of embryonic malformation and inhibited the embryonic growth. The addition of methionine increased the incidence of embryonic malformation at 10 microM of selenite but decreased the incidence of embryonic malformation at 100 microM of selenate. On the other hand, the addition of methionine partially restored the inhibited embryonic growth at 20 microM of selenite or at 100 microM of selenate. It was considered from these results that the methionine availability in the embryonic environment and the oxidation state of Se are critical in Se embryotoxicity.

[1]  R. Brent,et al.  Effects of supplemental methionine on antiserum-induced dysmorphology in rat embryos cultured in vitro. , 2000, Teratology.

[2]  R. Brent,et al.  Effects of methionine supplement on methionine incorporation in rat embryos cultured in vitro. , 1999, Teratology.

[3]  M. Usami,et al.  Effects of ascorbic acid on selenium teratogenicity in cultured rat embryos. , 1999, Toxicology letters.

[4]  S. Bonassi,et al.  L-methionine induces stage-dependent changes of differentiation and oxidative activity in sea urchin embryogenesis. , 1997, Pharmacology & toxicology.

[5]  E. Stadtman,et al.  Methionine residues as endogenous antioxidants in proteins. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[6]  I. Palmer,et al.  Selenium detoxification by methylation. , 1995, Research communications in molecular pathology and pharmacology.

[7]  J. Noordhoek,et al.  Prevention of neural tube defects by and toxicity of L-homocysteine in cultured postimplantation rat embryos. , 1994, Teratology.

[8]  J. M. Baden,et al.  Methionine Prevents Nitrous Oxide‐induced Teratogenicity in Rat Embryos Grown in Culture , 1994, Anesthesiology.

[9]  C. Willhite.  Selenium Teratogenesis Species‐Dependent Response and Influence on Reproduction , 1993, Annals of the New York Academy of Sciences.

[10]  N. W. Klein,et al.  Methionine decreases the embryotoxicity of sodium valproate in the rat: in vivo and in vitro observations. , 1992, Teratology.

[11]  G. Pendleton,et al.  Interactive effects of selenium, methionine, and dietary protein on survival, growth, and physiology in mallard ducklings , 1992, Archives of environmental contamination and toxicology.

[12]  J. Weber,et al.  Whole rat embryos require methionine for neural tube closure when cultured on cow serum. , 1989, The Journal of nutrition.

[13]  T. Flynn,et al.  Methionine and iron as growth factors for rat embryos cultured in canine serum. , 1987, The Journal of experimental zoology.

[14]  D. Hoffman,et al.  Reproduction in mallards fed selenium , 1987 .

[15]  J. Högberg,et al.  Effects of selenite on O2 consumption, glutathione oxidation and NADPH levels in isolated hepatocytes and the role of redox changes in selenite toxicity. , 1984, Chemico-biological interactions.

[16]  H. Satoh,et al.  Toxic effects of sodium selenite on pregnant mice and modification of the effects by vitamin E or reduced glutathione. , 1983, Teratology.

[17]  J. Högberg,et al.  Involvement of glutathione reductase in selenite metabolism and toxicity, studied in isolated rat hepatocytes , 1982, Archives of Toxicology.

[18]  S. Fabro,et al.  Quantitation of rat embryonic development in vitro: a morphological scoring system. , 1981, Teratology.

[19]  C. Chatot,et al.  Successful culture of rat embryos on human serum: use in the detection of teratogens. , 1980, Science.

[20]  S. Orrenius,et al.  The role of methionine in glutathione biosynthesis by isolated hepatocytes. , 1977, Biochemical and biophysical research communications.

[21]  I. Palmer,et al.  Toxicity of various selenium derivatives to chick embryos. , 1973, Poultry science.

[22]  O. Levander,et al.  Interactions of methionine, vitamin E, and antioxidants in selenium toxicity in the rat. , 1970, The Journal of nutrition.

[23]  D. D. Fischer,et al.  Identification of a major selenium excretory product in rat urine. , 1969, Biochimica et biophysica acta.

[24]  H. Ganther.  Enzymic synthesis of dimethyl selenide from sodium selenite in mouse liver extracts. , 1966, Biochemistry.

[25]  D. Karnofsky,et al.  THE EFFECTS OF METALS ON THE CHICK EMBRYO: TOXICITY AND PRODUCTION OF ABNORMALITIES IN DEVELOPMENT , 1952, Annals of the New York Academy of Sciences.

[26]  O. Portman,et al.  Excretion of dimethyl selenide by the rat. , 1952, The Journal of biological chemistry.

[27]  W. Poley,et al.  Monstrosities produced by the injection of selenium salts into hens' eggs , 1936 .

[28]  M. Usami,et al.  Effects of glutathione depletion on selenite- and selenate-induced embryotoxicity in cultured rat embryos. , 1999, Teratogenesis, carcinogenesis, and mutagenesis.

[29]  M. Usami,et al.  Teratogenic effects of selenium compounds on cultured postimplantation rat embryos. , 1996, Teratogenesis, carcinogenesis, and mutagenesis.

[30]  H. Nau,et al.  Methionine reduces the valproic acid-induced spina bifida rate in mice without altering valproic acid kinetics. , 1996, The Journal of nutrition.

[31]  S A Book,et al.  Developmental toxicity of L-selenomethionine in Macaca fascicularis. , 1991, Fundamental and applied toxicology : official journal of the Society of Toxicology.

[32]  W. Choy,et al.  Embryotoxicity and dose-response relationships of selenium in hamsters. , 1990, Reproductive toxicology.

[33]  D. Hoffman,et al.  Embryotoxic and teratogenic effects of selenium in the diet of mallards. , 1988, Journal of toxicology and environmental health.