Efficient sugar release by the cellulose solvent-based lignocellulose fractionation technology and enzymatic cellulose hydrolysis.

Efficient liberation of fermentable soluble sugars from lignocellulosic biomass waste not only decreases solid waste handling but also produces value-added biofuels and biobased products. Industrial hemp, a special economic crop, is cultivated for its high-quality fibers and high-value seed oil, but its hollow stalk cords (hurds) are a cellulosic waste. The cellulose-solvent-based lignocellulose fractionation (CSLF) technology has been developed to separate lignocellulose components under modest reaction conditions (Zhang, Y.-H. P.; Ding, S.-Y.; Mielenz, J. R.; Elander, R.; Laser, M.; Himmel, M.; McMillan, J. D.; Lynd, L. R. Biotechnol. Bioeng. 2007, 97 (2), 214- 223). Three pretreatment conditions (acid concentration, reaction temperature, and reaction time) were investigated to treat industrial hemp hurds for a maximal sugar release: a combinatorial result of a maximal retention of solid cellulose and a maximal enzymatic cellulose hydrolysis. At the best treatment condition (84.0% H3PO4 at 50 degrees C for 60 min), the glucan digestibility was 96% at hour 24 at a cellulase loading of 15 filter paper units of cellulase per gram of glucan. The scanning electron microscopic images were presented for the CSLF-pretreated biomass for the first time, suggesting that CSLF can completely destruct the plant cell-wall structure, in a good agreement with the highest enzymatic cellulose digestibility and fastest hydrolysis rate. It was found that phosphoric acid only above a critical concentration (83%) with a sufficient reaction time can efficiently disrupt recalcitrant lignocellulose structures.

[1]  G. T. Tsao,et al.  Cellulose to Sugars: New Path Gives Quantitative Yield , 1978, Science.

[2]  G. Weiblen,et al.  Genetic Variation in Hemp and Marijuana (Cannabis sativa L.) According to Amplified Fragment Length Polymorphisms * , 2006, Journal of forensic sciences.

[3]  Alistair King,et al.  Dissolution of wood in ionic liquids. , 2007, Journal of agricultural and food chemistry.

[4]  Charles E Wyman,et al.  What is (and is not) vital to advancing cellulosic ethanol. , 2007, Trends in biotechnology.

[5]  J. E. Stone,et al.  Digestibility as a Simple Function of a Molecule of Similar Size to a Cellulase Enzyme , 1969 .

[6]  N. Reddy,et al.  Biofibers from agricultural byproducts for industrial applications. , 2005, Trends in biotechnology.

[7]  Mark Holtzapple,et al.  Coordinated development of leading biomass pretreatment technologies. , 2005, Bioresource technology.

[8]  Jerome F. Saeman,et al.  Kinetics of Wood Saccharification - Hydrolysis of Cellulose and Decomposition of Sugars in Dilute Acid at High Temperature , 1945 .

[9]  N. Mosier,et al.  Microscopic examination of changes of plant cell structure in corn stover due to hot water pretreatment and enzymatic hydrolysis , 2007, Biotechnology and bioengineering.

[10]  Lee R Lynd,et al.  Quantification of cell and cellulase mass concentrations during anaerobic cellulose fermentation: development of an enzyme-linked immunosorbent assay-based method with application to Clostridium thermocellum batch cultures. , 2003, Analytical chemistry.

[11]  David K. Johnson,et al.  Biomass Recalcitrance: Engineering Plants and Enzymes for Biofuels Production , 2007, Science.

[12]  Lee R Lynd,et al.  Enzyme–microbe synergy during cellulose hydrolysis by Clostridium thermocellum , 2006, Proceedings of the National Academy of Sciences.

[13]  L. Lynd,et al.  Toward an aggregated understanding of enzymatic hydrolysis of cellulose: Noncomplexed cellulase systems , 2004, Biotechnology and bioengineering.

[14]  A. Gutiérrez,et al.  Chemical characterization of lignin and lipid fractions in industrial hemp bast fibers used for manufacturing high-quality paper pulps. , 2006, Journal of agricultural and food chemistry.

[15]  I. S. Pretorius,et al.  Microbial Cellulose Utilization: Fundamentals and Biotechnology , 2002, Microbiology and Molecular Biology Reviews.

[16]  L. Fan,et al.  The nature of lignocellulosics and their pretreatments for enzymatic hydrolysis , 1982 .

[17]  L. Lynd,et al.  A functionally based model for hydrolysis of cellulose by fungal cellulase , 2006, Biotechnology and bioengineering.

[18]  Shiro Saka,et al.  Bioconversion of hybrid poplar to ethanol and co‐products using an organosolv fractionation process: Optimization of process yields , 2006, Biotechnology and bioengineering.

[19]  T. Kim,et al.  Pretreatment and fractionation of corn stover by ammonia recycle percolation process. , 2005, Bioresource technology.

[20]  David J. Gregg,et al.  Strategies to enhance the enzymatic hydrolysis of pretreated softwood with high residual lignin content , 2005, Applied biochemistry and biotechnology.

[21]  L. Lynd,et al.  How biotech can transform biofuels , 2008, Nature Biotechnology.

[22]  Ziniu Yu,et al.  Dissolution of cellulose with ionic liquids and its application : a mini-review , 2006 .

[23]  James D. McMillan,et al.  Pretreatment of lignocellulosic biomass , 1994 .

[24]  M. Chang,et al.  Structure, pretreatment and hydrolysis of cellulose , 1981 .

[25]  L. Lynd,et al.  Biocommodity Engineering , 1999, Biotechnology progress.

[26]  Y.‐H.P. Zhang,et al.  More Accurate Determination of Acid-Labile Carbohydrates in Lignocellulose by Modified Quantitative Saccharification , 2007 .

[27]  Y.‐H.P. Zhang.  Reviving the carbohydrate economy via multi-product lignocellulose biorefineries , 2008, Journal of Industrial Microbiology & Biotechnology.

[28]  Xinhao Ye,et al.  Quantitative determination of cellulose accessibility to cellulase based on adsorption of a nonhydrolytic fusion protein containing CBM and GFP with its applications. , 2007, Langmuir : the ACS journal of surfaces and colloids.

[29]  Mark Holtzapple,et al.  Comparative sugar recovery data from laboratory scale application of leading pretreatment technologies to corn stover. , 2005, Bioresource technology.

[30]  C. Wyman,et al.  Characterization of molecular weight distribution of oligomers from autocatalyzed batch hydrolysis of xylan , 2003, Applied biochemistry and biotechnology.

[31]  M. Himmel,et al.  Deposition of Lignin Droplets Produced During Dilute Acid Pretreatment of Maize Stems Retards Enzymatic Hydrolysis of Cellulose , 2007, Biotechnology progress.

[32]  M. Himmel,et al.  Outlook for cellulase improvement: screening and selection strategies. , 2006, Biotechnology advances.

[33]  Lee R Lynd,et al.  A transition from cellulose swelling to cellulose dissolution by o-phosphoric acid: evidence from enzymatic hydrolysis and supramolecular structure. , 2006, Biomacromolecules.

[34]  Mark Laser,et al.  Fractionating recalcitrant lignocellulose at modest reaction conditions. , 2007, Biotechnology and bioengineering.

[35]  C. A. Schall,et al.  Enhancement of cellulose saccharification kinetics using an ionic liquid pretreatment step , 2006, Biotechnology and bioengineering.

[36]  Lee R. Lynd,et al.  Regulation of Cellulase Synthesis in Batch and Continuous Cultures of Clostridium thermocellum , 2005, Journal of bacteriology.

[37]  Charlotte K. Williams,et al.  The Path Forward for Biofuels and Biomaterials , 2006, Science.

[38]  Xiaoquan Yang,et al.  Properties of cast films from hemp (Cannabis sativa L.) and soy protein isolates. A comparative study. , 2007, Journal of agricultural and food chemistry.

[39]  Y.‐H.P. Zhang,et al.  Bioseparation of recombinant cellulose-binding module-proteins by affinity adsorption on an ultra-high-capacity cellulosic adsorbent. , 2008, Analytica chimica acta.

[40]  Daniel J Schell,et al.  Methodological analysis for determination of enzymatic digestibility of cellulosic materials , 2007, Biotechnology and bioengineering.