Breaking barriers to transcription elongation

Hundreds of protein factors participate in transcription and its regulation in eukaryotes. Many of these proteins regulate specific genes by targeting upstream promoter regions, whereas a smaller but mechanistically diverse set of factors functions at most genes during RNA polymerase II (Pol II) elongation. These elongation factors can affect mRNA production at particular stages and in different ways during transcription. Some factors act directly on Pol II, whereas others manipulate the chromatin environment.

[1]  Saeed Tavazoie,et al.  Mapping Global Histone Acetylation Patterns to Gene Expression , 2004, Cell.

[2]  D. Reinberg,et al.  Recycling of the general transcription factors during RNA polymerase II transcription. , 1995, Genes & development.

[3]  K. Yano,et al.  Human Transcription Elongation Factor NELF: Identification of Novel Subunits and Reconstitution of the Functionally Active Complex , 2003, Molecular and Cellular Biology.

[4]  Kevin Struhl,et al.  Targeted recruitment of Set1 histone methylase by elongating Pol II provides a localized mark and memory of recent transcriptional activity. , 2003, Molecular cell.

[5]  D. Reinberg,et al.  Human Elongator facilitates RNA polymerase II transcription through chromatin , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[6]  James T Kadonaga,et al.  Chromatin remodeling by ATP-dependent molecular machines. , 2003, BioEssays : news and reviews in molecular, cellular and developmental biology.

[7]  G. Prelich.  RNA Polymerase II Carboxy-Terminal Domain Kinases: Emerging Clues to Their Function , 2002, Eukaryotic Cell.

[8]  Kevin Struhl,et al.  The FACT Complex Travels with Elongating RNA Polymerase II and Is Important for the Fidelity of Transcriptional Initiation In Vivo , 2003, Molecular and Cellular Biology.

[9]  L. Hsu,et al.  Promoter clearance and escape in prokaryotes. , 2002, Biochimica et biophysica acta.

[10]  D. Reinberg,et al.  Functional interactions of RNA-capping enzyme with factors that positively and negatively regulate promoter escape by RNA polymerase II. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[11]  Patrick Cramer,et al.  RNA polymerase II structure: from core to functional complexes. , 2004, Current opinion in genetics & development.

[12]  A. Rougvie,et al.  The RNA polymerase II molecule at the 5′ end of the uninduced hsp70 gene of D. melanogaster is transcriptionally engaged , 1988, Cell.

[13]  Brian D. Strahl,et al.  Dimethylation of Histone H3 at Lysine 36 DemarcatesRegulatory and Nonregulatory ChromatinGenome-Wide , 2005, Molecular and Cellular Biology.

[14]  S. Berger,et al.  Phosphorylation of serine 10 in histone H3 is functionally linked in vitro and in vivo to Gcn5-mediated acetylation at lysine 14. , 2000, Molecular cell.

[15]  M. J. Barratt,et al.  Phosphoacetylation of histone H3 on c‐fos‐ and c‐jun‐associated nucleosomes upon gene activation , 2000, The EMBO journal.

[16]  R. Ho,et al.  The elongation factors Pandora/Spt6 and Foggy/Spt5 promote transcription in the zebrafish embryo. , 2002, Development.

[17]  Brian D. Strahl,et al.  A Novel Domain in Set2 Mediates RNA Polymerase II Interaction and Couples Histone H3 K36 Methylation with Transcript Elongation , 2005, Molecular and Cellular Biology.

[18]  D. Eick,et al.  Transcriptional regulation of the Ig kappa gene by promoter-proximal pausing of RNA polymerase II. , 1999, Journal of immunology.

[19]  J. Lis,et al.  Transcription Factor and Polymerase Recruitment, Modification, and Movement on dhsp70 In Vivo in the Minutes following Heat Shock , 2003, Molecular and Cellular Biology.

[20]  Paul Tempst,et al.  The human PAF complex coordinates transcription with events downstream of RNA synthesis. , 2005, Genes & development.

[21]  G. Orphanides,et al.  FACT Facilitates Transcription-Dependent Nucleosome Alteration , 2003, Science.

[22]  E. Cho,et al.  Different phosphorylated forms of RNA polymerase II and associated mRNA processing factors during transcription. , 2000, Genes & development.

[23]  D. Reinberg,et al.  Histone H2B Monoubiquitination Functions Cooperatively with FACT to Regulate Elongation by RNA Polymerase II , 2006, Cell.

[24]  Steven J Altschuler,et al.  Genomic characterization reveals a simple histone H4 acetylation code. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[25]  Mahadeb Pal,et al.  The role of the transcription bubble and TFIIB in promoter clearance by RNA polymerase II. , 2005, Molecular cell.

[26]  S. Shuman,et al.  Interactions between Fission Yeast Cdk9, Its Cyclin Partner Pch1, and mRNA Capping Enzyme Pct1 Suggest an Elongation Checkpoint for mRNA Quality Control* , 2003, The Journal of Biological Chemistry.

[27]  A. Shilatifard,et al.  Drosophila ELL is associated with actively elongating RNA polymerase II on transcriptionally active sites in vivo , 2001, The EMBO journal.

[28]  Roger D Kornberg,et al.  Structural Basis of Transcription: An RNA Polymerase II-TFIIB Cocrystal at 4.5 Angstroms , 2004, Science.

[29]  D. Gilmour,et al.  Pcf11 is a termination factor in Drosophila that dismantles the elongation complex by bridging the CTD of RNA polymerase II to the nascent transcript. , 2006, Molecular cell.

[30]  D. Bentley,et al.  A Ribonucleolytic Rat Torpedoes RNA Polymerase II , 2004, Cell.

[31]  J. Lis,et al.  DNA melting on yeast RNA polymerase II promoters. , 1993, Science.

[32]  C. Ponting,et al.  Regulation of chromatin structure by site-specific histone H3 methyltransferases , 2000, Nature.

[33]  Craig D. Kaplan,et al.  Interaction between Transcription Elongation Factors and mRNA 3′-End Formation at the Saccharomyces cerevisiae GAL10-GAL7 Locus* , 2005, Journal of Biological Chemistry.

[34]  Peter A. Jones,et al.  Distinct localization of histone H3 acetylation and H3-K4 methylation to the transcription start sites in the human genome. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[35]  G. Michelotti,et al.  Evidence That Phosphorylation of the RNA Polymerase II Carboxyl-terminal Repeats Is Similar in Yeast and Humans* , 2005, Journal of Biological Chemistry.

[36]  Zu-Wen Sun,et al.  Ubiquitination of histone H2B regulates H3 methylation and gene silencing in yeast , 2002, Nature.

[37]  D. Luse,et al.  RNA Polymerase II Transcription Complexes May Become Arrested If the Nascent RNA Is Shortened to Less than 50 Nucleotides* , 2002, The Journal of Biological Chemistry.

[38]  A. Aguilera,et al.  Molecular evidence for a positive role of Spt4 in transcription elongation , 2003, The EMBO journal.

[39]  H. Handa,et al.  A regulator of transcriptional elongation controls vertebrate neuronal development , 2000, Nature.

[40]  T. Kundu,et al.  Human Histone Chaperone Nucleophosmin Enhances Acetylation-Dependent Chromatin Transcription , 2005, Molecular and Cellular Biology.

[41]  C. Allis,et al.  Synergistic coupling of histone H3 phosphorylation and acetylation in response to epidermal growth factor stimulation. , 2000, Molecular cell.

[42]  Tony Kouzarides,et al.  Histone H3 lysine 4 methylation patterns in higher eukaryotic genes , 2004, Nature Cell Biology.

[43]  Jeffrey W. Roberts,et al.  Antitermination by bacteriophage lambda Q protein. , 1998, Cold Spring Harbor symposia on quantitative biology.

[44]  Karl Henry,et al.  Rad6 plays a role in transcriptional activation through ubiquitylation of histone H2B. , 2004, Genes & development.

[45]  J. Lis,et al.  Efficient release from promoter-proximal stall sites requires transcript cleavage factor TFIIS. , 2005, Molecular cell.

[46]  Leah Barrera,et al.  A high-resolution map of active promoters in the human genome , 2005, Nature.

[47]  J. Manley,et al.  The mammalian RNA polymerase II C-terminal domain interacts with RNA to suppress transcription-coupled 3' end formation. , 2005, Molecular cell.

[48]  Chintamani,et al.  Qualitative and quantitative dermatoglyphic traits in patients with breast cancer: a prospective clinical study , 2007, BMC Cancer.

[49]  R. G. Keene,et al.  Initially Transcribed Sequences Strongly Affect the Extent of Abortive Initiation by RNA Polymerase II* , 1999, The Journal of Biological Chemistry.

[50]  J. Lis,et al.  Coordination of transcription, RNA processing, and surveillance by P-TEFb kinase on heat shock genes. , 2004, Molecular cell.

[51]  N. Krogan,et al.  Histone H2B Ubiquitylation Is Associated with Elongating RNA Polymerase II , 2005, Molecular and Cellular Biology.

[52]  M. Hampsey,et al.  Functional Interaction between TFIIB and the Rpb2 Subunit of RNA Polymerase II: Implications for the Mechanism of Transcription Initiation , 2004, Molecular and Cellular Biology.

[53]  Hiroshi Handa,et al.  P-TEFb-mediated phosphorylation of hSpt5 C-terminal repeats is critical for processive transcription elongation. , 2006, Molecular cell.

[54]  J. R. Morris,et al.  Spt5 and spt6 are associated with active transcription and have characteristics of general elongation factors in D. melanogaster. , 2000, Genes & development.

[55]  J. M. Lee,et al.  Locus-specific variation in phosphorylation state of RNA polymerase II in vivo: correlations with gene activity and transcript processing. , 1993, Genes & development.

[56]  V. Corces,et al.  Protein Phosphatase 2A Activity Affects Histone H3 Phosphorylation and Transcription in Drosophila melanogaster , 2003, Molecular and Cellular Biology.

[57]  Nicholas Proudfoot,et al.  Isw1 Chromatin Remodeling ATPase Coordinates Transcription Elongation and Termination by RNA Polymerase II , 2003, Cell.

[58]  D. Luse,et al.  RNA emerging from the active site of RNA polymerase II interacts with the Rpb7 subunit , 2006, Nature Structural &Molecular Biology.

[59]  R. Perry,et al.  CHD1 is concentrated in interbands and puffed regions of Drosophila polytene chromosomes. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[60]  Christoph H Borchers,et al.  Phosphorylation of RNA polymerase II CTD regulates H3 methylation in yeast. , 2003, Genes & development.

[61]  Megan F. Cole,et al.  Genome-wide Map of Nucleosome Acetylation and Methylation in Yeast , 2005, Cell.

[62]  A. Rougvie,et al.  Postinitiation transcriptional control in Drosophila melanogaster , 1990, Molecular and cellular biology.

[63]  J. Tamkun,et al.  The Drosophila trithorax group protein Kismet facilitates an early step in transcriptional elongation by RNA Polymerase II , 2005, Development.

[64]  J. Lis,et al.  In vivo transcriptional pausing and cap formation on three Drosophila heat shock genes. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[65]  R. Conaway,et al.  Dual Roles for Transcription Factor IIF in Promoter Escape by RNA Polymerase II* , 1999, The Journal of Biological Chemistry.

[66]  R. Conaway,et al.  Mechanism of Action of RNA Polymerase II Elongation Factor Elongin , 1998, The Journal of Biological Chemistry.

[67]  J. Goodrich,et al.  The Sequence at Specific Positions in the Early Transcribed Region Sets the Rate of Transcript Synthesis by RNA Polymerase II in Vitro* , 2005, Journal of Biological Chemistry.

[68]  Bing Li,et al.  Histone H3 Methylation by Set2 Directs Deacetylation of Coding Regions by Rpd3S to Suppress Spurious Intragenic Transcription , 2005, Cell.

[69]  G. Cagney,et al.  Methylation of Histone H3 by Set2 in Saccharomyces cerevisiae Is Linked to Transcriptional Elongation by RNA Polymerase II , 2003, Molecular and Cellular Biology.

[70]  D. S. Gross,et al.  Domain-Wide Displacement of Histones by Activated Heat Shock Factor Occurs Independently of Swi/Snf and Is Not Correlated with RNA Polymerase II Density , 2005, Molecular and Cellular Biology.

[71]  R. Landick,et al.  A negative elongation factor for human RNA polymerase II inhibits the anti-arrest transcript-cleavage factor TFIIS. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[72]  H. Cai,et al.  Transcription initiation by RNA polymerase II in vitro. Properties of preinitiation, initiation, and elongation complexes. , 1987, The Journal of biological chemistry.

[73]  Michael Grunstein,et al.  Requirement of Hos2 Histone Deacetylase for Gene Activity in Yeast , 2002, Science.

[74]  Thomas A. Milne,et al.  Physical Association and Coordinate Function of the H3 K4 Methyltransferase MLL1 and the H4 K16 Acetyltransferase MOF , 2005, Cell.

[75]  M. Scott,et al.  The Drosophila BRM complex facilitates global transcription by RNA polymerase II , 2002, The EMBO journal.

[76]  G. Kay,et al.  Menin associates with a trithorax family histone methyltransferase complex and with the hoxc8 locus. , 2004, Molecular cell.

[77]  X. Graña,et al.  Cellular control of gene expression by T-type cyclin/CDK9 complexes. , 2004, Gene.

[78]  M. Merville,et al.  Transcription impairment and cell migration defects in elongator-depleted cells: implication for familial dysautonomia. , 2006, Molecular cell.

[79]  R. Kingston,et al.  Localized recruitment of a chromatin-remodeling activity by an activator in vivo drives transcriptional elongation. , 2003, Genes & development.

[80]  J. Wang,et al.  Drosophila DNA topoisomerase I is associated with transcriptionally active regions of the genome. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[81]  Phan T. Tran,et al.  Dissociable Rpb4-Rpb7 Subassembly of RNA Polymerase II Binds to Single-strand Nucleic Acid and Mediates a Post-recruitment Step in Transcription Initiation* , 2001, The Journal of Biological Chemistry.

[82]  A. Shatkin,et al.  Transcription elongation factor hSPT5 stimulates mRNA capping. , 1999, Genes & development.

[83]  F. Winston,et al.  Evidence that Spt4, Spt5, and Spt6 control transcription elongation by RNA polymerase II in Saccharomyces cerevisiae. , 1998, Genes & development.

[84]  C. Moore,et al.  Kin28, the TFIIH-Associated Carboxy-Terminal Domain Kinase, Facilitates the Recruitment of mRNA Processing Machinery to RNA Polymerase II , 2000, Molecular and Cellular Biology.

[85]  Richard A Young,et al.  Global and Hox-specific roles for the MLL1 methyltransferase. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[86]  K. Struhl,et al.  ArticleAsf 1 Mediates Histone Eviction and Deposition during Elongation by RNA Polymerase II , 2022 .

[87]  J. Goodrich,et al.  An 8 nt RNA triggers a rate‐limiting shift of RNA polymerase II complexes into elongation , 2006, The EMBO journal.

[88]  H. Handa,et al.  Molecular characterization of Drosophila NELF , 2005, Nucleic acids research.

[89]  D. Reinberg,et al.  Drosophila Paf1 Modulates Chromatin Structure at Actively Transcribed Genes , 2006, Molecular and Cellular Biology.

[90]  Brian D. Strahl,et al.  Gene silencing: Trans-histone regulatory pathway in chromatin , 2002, Nature.

[91]  Hien G. Tran,et al.  Chromatin remodeling protein Chd1 interacts with transcription elongation factors and localizes to transcribed genes , 2003, The EMBO journal.

[92]  Richard A Young,et al.  Exchange of RNA polymerase II initiation and elongation factors during gene expression in vivo. , 2002, Molecular cell.

[93]  Yang Shi,et al.  Histone Demethylation Mediated by the Nuclear Amine Oxidase Homolog LSD1 , 2004, Cell.

[94]  D. Bushnell,et al.  Structural Basis of Transcription: Separation of RNA from DNA by RNA Polymerase II , 2004, Science.

[95]  Yi Zhang,et al.  Bre1, an E3 ubiquitin ligase required for recruitment and substrate selection of Rad6 at a promoter. , 2003, Molecular cell.

[96]  S. Henikoff,et al.  Assembly of variant histones into chromatin. , 2005, Annual review of cell and developmental biology.

[97]  J. Lis,et al.  RNA polymerase II interacts with the promoter region of the noninduced hsp70 gene in Drosophila melanogaster cells. , 1986, Molecular and cellular biology.

[98]  D. Price.  P-TEFb, a Cyclin-Dependent Kinase Controlling Elongation by RNA Polymerase II , 2000, Molecular and Cellular Biology.

[99]  B. Peterlin,et al.  P‐TEFb is not an essential elongation factor for the intronless human U2 snRNA and histone H2b genes , 2005, The EMBO journal.

[100]  R. Conaway,et al.  Transcription elongation and human disease. , 1999, Annual review of biochemistry.

[101]  Steven Hahn,et al.  Structure and mechanism of the RNA polymerase II transcription machinery , 2004, Nature Structural &Molecular Biology.

[102]  Craig D. Kaplan,et al.  Transcription Elongation Factors Repress Transcription Initiation from Cryptic Sites , 2003, Science.

[103]  Anton Meinhart,et al.  A structural perspective of CTD function. , 2005, Genes & development.

[104]  J. Svejstrup.  The RNA polymerase II transcription cycle: cycling through chromatin. , 2004, Biochimica et biophysica acta.

[105]  J. Lis,et al.  P-TEFb kinase recruitment and function at heat shock loci. , 2000, Genes & development.

[106]  B. Cairns,et al.  Transcriptional inhibition of genes with severe histone h3 hypoacetylation in the coding region. , 2002, Molecular cell.

[107]  S. Khorasanizadeh.  The Nucleosome From Genomic Organization to Genomic Regulation , 2004, Cell.

[108]  F. Winston,et al.  Evidence That Spt6p Controls Chromatin Structure by a Direct Interaction with Histones , 1996, Science.

[109]  B. Peterlin,et al.  A New Paradigm in Eukaryotic Biology: HIV Tat and the Control of Transcriptional Elongation , 2005, PLoS biology.

[110]  Kevin Struhl,et al.  Asf1 mediates histone eviction and deposition during elongation by RNA polymerase II. , 2006, Molecular cell.

[111]  J. Gralla,et al.  Polymerase II promoter activation: closed complex formation and ATP-driven start site opening. , 1992, Science.

[112]  Yang Shi,et al.  CDK-9/cyclin T (P-TEFb) is required in two postinitiation pathways for transcription in the C. elegans embryo. , 2002, Genes & development.

[113]  Paul Tempst,et al.  Monoubiquitination of human histone H2B: the factors involved and their roles in HOX gene regulation. , 2005, Molecular cell.

[114]  Xing Zhang,et al.  The SET-domain protein superfamily: protein lysine methyltransferases , 2005, Genome Biology.

[115]  Denys A. Khaperskyy,et al.  A Functional Role for the Switch 2 Region of Yeast RNA Polymerase II in Transcription Start Site Utilization and Abortive Initiation* , 2005, Journal of Biological Chemistry.

[116]  M. Osley,et al.  Rad6-dependent ubiquitination of histone H2B in yeast. , 2000, Science.

[117]  Charles Boone,et al.  A conserved RING finger protein required for histone H2B monoubiquitination and cell size control. , 2003, Molecular cell.

[118]  J. Greenblatt,et al.  Opposing effects of Ctk 1 kinase and Fcp 1 phosphatase at Ser 2 of the RNA polymerase II C-terminal domain , 2001 .

[119]  D. Reinberg,et al.  Tracking FACT and the RNA Polymerase II Elongation Complex Through Chromatin in Vivo , 2003, Science.

[120]  Kevin Struhl,et al.  Ubiquitination of Histone H2B by Rad6 Is Required for Efficient Dot1-mediated Methylation of Histone H3 Lysine 79* , 2002, The Journal of Biological Chemistry.

[121]  A. Dvir,et al.  Promoter escape by RNA polymerase II. , 2002, Biochimica et biophysica acta.

[122]  F. Holstege,et al.  Three transitions in the RNA polymerase II transcription complex during initiation , 1997, The EMBO journal.

[123]  Andrew Flaus,et al.  Histone H2A/H2B dimer exchange by ATP-dependent chromatin remodeling activities. , 2003, Molecular cell.

[124]  J. Greenblatt,et al.  Opposing effects of Ctk1 kinase and Fcp1 phosphatase at Ser 2 of the RNA polymerase II C-terminal domain. , 2001, Genes & development.

[125]  S. Tillib,et al.  Modulation of heat shock gene expression by the TAC1 chromatin-modifying complex , 2004, Nature Cell Biology.

[126]  Mihail Sarov,et al.  The histone 3 lysine 36 methyltransferase, SET2, is involved in transcriptional elongation. , 2003, Nucleic acids research.

[127]  A. Furger,et al.  Integrating mRNA Processing with Transcription , 2002, Cell.

[128]  J. Lis,et al.  High-resolution localization of Drosophila Spt5 and Spt6 at heat shock genes in vivo: roles in promoter proximal pausing and transcription elongation. , 2000, Genes & development.

[129]  A. Shilatifard,et al.  Regulation of Heat Shock Gene Expression by RNA Polymerase II Elongation Factor, Elongin A* , 2005, Journal of Biological Chemistry.

[130]  J. Lis,et al.  Promoter-associated pausing in promoter architecture and postinitiation transcriptional regulation. , 1998, Cold Spring Harbor symposia on quantitative biology.

[131]  D. Reinberg,et al.  Human Spt6 Stimulates Transcription Elongation by RNA Polymerase II In Vitro , 2004, Molecular and Cellular Biology.

[132]  Mark Johnston,et al.  The Paf1 Complex Is Essential for Histone Monoubiquitination by the Rad6-Bre1 Complex, Which Signals for Histone Methylation by COMPASS and Dot1p* , 2003, Journal of Biological Chemistry.

[133]  N. Friedman,et al.  Single-Nucleosome Mapping of Histone Modifications in S. cerevisiae , 2005, PLoS biology.

[134]  B. Peterlin,et al.  Dynamics of Human Immunodeficiency Virus Transcription: P-TEFb Phosphorylates RD and Dissociates Negative Effectors from the Transactivation Response Element , 2004, Molecular and Cellular Biology.

[135]  J. Lis,et al.  Promoter melting and TFIID complexes on Drosophila genes in vivo. , 1992, Genes & development.

[136]  Danny Reinberg,et al.  Elongation by RNA polymerase II: the short and long of it. , 2004, Genes & development.

[137]  V. Honti,et al.  Characterization of the grappa Gene, the Drosophila Histone H3 Lysine 79 Methyltransferase , 2005, Genetics.

[138]  P. Hanawalt,et al.  The anti-cancer drug camptothecin inhibits elongation but stimulates initiation of RNA polymerase II transcription. , 1996, Carcinogenesis.

[139]  Erratum: Chromatin disassembly mediated by the histone chaperone asf1 is essential for transcriptional activation of the yeast PHO5 and PHO8 genes (Molecular Cell (June 4, 2004) 14 (657-666)) , 2004 .

[140]  J Seth Strattan,et al.  Removal of promoter nucleosomes by disassembly rather than sliding in vivo. , 2004, Molecular cell.

[141]  V. Iyer,et al.  The chromo domain protein Chd1p from budding yeast is an ATP‐dependent chromatin‐modifying factor , 2000, The EMBO journal.

[142]  Mark Johnston,et al.  Methylation of Histone H3 by COMPASS Requires Ubiquitination of Histone H2B by Rad6* , 2002, The Journal of Biological Chemistry.

[143]  D. Price,et al.  Flavopiridol Inactivates P-TEFb and Blocks Most RNA Polymerase II Transcription in Vivo * , 2001, The Journal of Biological Chemistry.

[144]  J. Goodrich,et al.  A Kinetic Model for the Early Steps of RNA Synthesis by Human RNA Polymerase II* , 2000, The Journal of Biological Chemistry.

[145]  H. Erdjument-Bromage,et al.  Multiple Mechanisms Confining RNA Polymerase II Ubiquitylation to Polymerases Undergoing Transcriptional Arrest , 2005, Cell.

[146]  Kevin Struhl,et al.  Distinction and relationship between elongation rate and processivity of RNA polymerase II in vivo. , 2005, Molecular cell.

[147]  J. Brady,et al.  The bromodomain protein Brd4 is a positive regulatory component of P-TEFb and stimulates RNA polymerase II-dependent transcription. , 2005, Molecular cell.

[148]  Mahadeb Pal,et al.  The initiation–elongation transition: Lateral mobility of RNA in RNA polymerase II complexes is greatly reduced at +8/+9 and absent by +23 , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[149]  C. Kane,et al.  Promoting elongation with transcript cleavage stimulatory factors. , 2002, Biochimica et biophysica acta.

[150]  M. Pazin,et al.  Histone H4-K16 Acetylation Controls Chromatin Structure and Protein Interactions , 2006, Science.

[151]  G. Cagney,et al.  RNA Polymerase II Elongation Factors of Saccharomyces cerevisiae: a Targeted Proteomics Approach , 2002, Molecular and Cellular Biology.

[152]  Andrés Aguilera,et al.  Cotranscriptionally formed DNA:RNA hybrids mediate transcription elongation impairment and transcription-associated recombination. , 2003, Molecular cell.

[153]  M. Johnston,et al.  The Paf1 complex is required for histone H3 methylation by COMPASS and Dot1p: linking transcriptional elongation to histone methylation. , 2003, Molecular cell.

[154]  N. Krogan,et al.  Histone H 2 B Ubiquitylation Is Associated with Elongating RNA Polymerase II , 2004 .