Action of secretagogues on a new preparation of functionally intact, isolated pancreatic acini.

Isolated pancreatic acini were prepared by a new method from mouse and rat pancreases by digestion with purified collagenase and chymotrypsin followed by mechanical shearing. Acini were structurally similar to those of the intact pancreas, having a normal luminal structure but with the basal acinar cell membranes exposed to the incubation medium. Amylase release in response to both cholinergic analogues and the cholecystokinin analogues caerulein and pentagastrin was comparable to that of the intact pancreas, but was much greater than previously reported for isolated acinar cells. Cholinergic-stimulated release was inhibited by atropine with a Ki value of 1.4 nM which is comparable to other muscarinic receptors. All agonists tested, when added at supramaximal concentrations, produced a submaximal release of amylase even though ATP levels and the release of slowly exchanging 45Ca2+ were normal or increased. Acini releasing amylase submaximally after being exposed to supramaximal concentrations of carbachol failed to respond to a maximal amount of caerulein or to the Ca2+ ionophore A23187. It is concluded that the decreased response (desensitization) is a postreceptor phenomenon and possibly mediated by Ca2+ itself.

[1]  D. Chandler.  Control of pancreatic enzyme secretion: a critique on the role of calcium. , 1978, Life sciences.

[2]  D. Chandler,et al.  Intracellular divalent cation release in pancreatic acinar cells during stimulus-secretion coupling. I. Use of chlorotetracycline as fluorescent probe , 1978, The Journal of cell biology.

[3]  P. D. Webster,et al.  Pancreatic acinar cell metabolism and function. , 1977, Gastroenterology.

[4]  J. Gardner,et al.  Regulation of amylase release from dispersed pancreatic acinar cells. , 1977, The Journal of physiology.

[5]  J. Putney.  Muscarinic, alpha‐adrenergic and peptide receptors regulate the same calcium influx sites in the parotid gland. , 1977, The Journal of physiology.

[6]  P. Robberecht,et al.  "In vitro" effects of secretin and vasoactive intestinal polypeptide on hydrolase secretion and cylic AMP levels in the pancreas of five animal species. A comparison with caerulein. , 1977, Gastroenterologie clinique et biologique.

[7]  P. Cary,et al.  Effects of ions on amylase release by dissociated pancreatic acinar cells. , 1976, The American journal of physiology.

[8]  J. Gardner,et al.  Action of cholecystokinin, cholinergic agents, and A-23187 on accumulation of guanosine 3':5'-monophosphate in dispersed guinea pig pancreatic acinar cells. , 1976, The Journal of biological chemistry.

[9]  J. Gardner,et al.  Interaction of porcine vasoactive intestinal peptide with dispersed pancreatic acinar cells from the guinea pig. Binding of radioiodinated peptide. , 1976, The Journal of biological chemistry.

[10]  S. Kondo,et al.  Calcium ion uptake in isolated pancreas cells induced by secretagogues. , 1976, Biochimica et biophysica acta.

[11]  J. Williams,et al.  An In Vitro Evaluation of Possible Cholinergic and Adrenergic Receptors Affecting Pancreatic Amylase Secretion , 1975, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[12]  George Palade,et al.  Intracellular Aspects of the Process of Protein Synthesis , 1975, Science.

[13]  J. Gardner,et al.  Action of cholecystokinin and cholinergic agents on calcium transport in isolated pancreatic acinar cells. , 1975, The Journal of clinical investigation.

[14]  A. Amsterdam,et al.  Studies on Dispersed Pancreatic Exocrine Cells I. Dissociation Technique and Morphologic Characteristics of Separated Cells , 1974 .

[15]  A. Amsterdam,et al.  Studies on dispersed pancreatic exocrine cells. II. Functional characteristics of separated cells. , 1974 .

[16]  John A. Williams,et al.  Pancreatic acinar cells: use of Ca++ ionophore to separate enzyme release from the earlier steps in stimulus-secretion coupling. , 1974, Biochemical and biophysical research communications.

[17]  D. J. Wolff,et al.  Calcium-binding phosphoprotein from pig brain: identification as a calcium-dependent regulator of brain cyclic nucleotide phosphodiesterase. , 1974, Archives of biochemistry and biophysics.

[18]  A Amsterdam,et al.  Structural and functional characterization of isolated pancreatic exocrine cells. , 1972, Proceedings of the National Academy of Sciences of the United States of America.

[19]  J. Dumont,et al.  Role of cyclic 3',5'-amp in the action of physiological secretagogues on the metabolism of rat pancreas in vitro. , 1971, Biochimica et biophysica acta.

[20]  G. Palade,et al.  CONDENSING VACUOLE CONVERSION AND ZYMOGEN GRANULE DISCHARGE IN PANCREATIC EXOCRINE CELLS: METABOLIC STUDIES , 1971, The Journal of cell biology.

[21]  P. Stanley,et al.  Use of the liquid scintillation spectrometer for determining adenosine triphosphate by the luciferase enzyme. , 1969, Analytical biochemistry.

[22]  J. Dumont,et al.  Energy sources for protein synthesis and enzymatic secretion in rat pancreas in vitro. , 1969, Biochimica et biophysica acta.

[23]  H. Rang,et al.  The uptake of atropine and related drugs by intestinal smooth muscle of the guinea-pig in relation to acetylcholine receptors , 1965, Proceedings of the Royal Society of London. Series B. Biological Sciences.

[24]  Oliver H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.

[25]  M. Korc,et al.  Evidence that calcium is a hormonal second messenger , 1978 .

[26]  J. E. Jorpes.  [Secretin and cholecystokinin]. , 1974, Tidsskrift for den Norske laegeforening : tidsskrift for praktisk medicin, ny raekke.