The Inhibition of Seedling Growth by Streptomycin Sulfate

The action of streptomycin sulfate (SS) on the growth of germinating seeds was investigated. It inhibited root growth more than shoot growth. Chemical analysis of roots indicated that nucleic acids decreased but their elution pattern from a column of methylated albumin kieselghur showed no qualitative differences. SS decreased the number of cells undergoing mitosis per root tip. This number was correlated with root length. One nM N5-benzyladenine reversed the effect of SS on both root length and mitosis. Thus the effect of SS appears specific to cell division. SS failed to affect oxidative phosphorylation in isolated mitochondria so its effect on cell division would not be secondary to the inhibition of energy conservation. It s possible inhibition of enzyme activity is selective since it also failed to affect RNase activity in vitro.

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