Evaluating effectiveness of ultracentrifugation for removal of interference caused by lipemia in estimation of amylase, urea, creatinine, glucose and uric acid

Introduction: Lipemic samples are affecting sample integrity and may raise questions on accuracy of test results. To overcome lipemic interference ultracentrifugation, high speed centrifugation, lipid clearing agent, dilution with normal saline or sample blanking can be used. Out of these, ultracentrifugation is likely to cause least interference as there is minimal exposure to additional chemicals. Aims and Objectives: The study was conducted to compare the results obtained for some commonly asked biochemical parameters, before and after ultracentrifugation and to assess the need of removal of lipemia using ultracentrifugation as a tool for these parameters. Materials and Methods: From 50 lipemic samples, 2 aliquots were prepared each having 500 microliter of serum. Out of these 2, one was used to perform amylase, urea, creatinine, glucose and uric acid. From Second aliquot, same parameters were performed after ultracentrifugation and comparison of results obtained, was done by Student’s t-test. Moreover, ratio of % lipemic bias to CLIA criteria, biological variation and % coefficient of variance of laboratory were also calculated to see whether the difference is clinically significant too. Results: Statistically significant difference between lipemic and ultracentrifuge samples was observed in amylase, urea, creatinine, uric acid and glucose while only glucose and uric acid showed clinically significant difference. Conclusion: Lipemia causes statistically significant interference in estimation of amylase, urea, creatinine, glucose and uric acid and ultracentrifugation is very effective process in removal interference caused by lipemia. When this statistically significant interference was studied in the light of clinical need, only glucose and uric acid showed clinically significant interference. Laboratories not equipped with ultracentrifuge, can use kinetic method, two point kinetic method or at least two reagents method as alternative. © 2019 Published by Innovative Publication. This is an open access article under the CC BY-NC-ND license (https://creativecommons.org/licenses/by/4.0/)