Optimal antigen retrieval for ethanol‐fixed cytologic smears

Antigen retrieval, a crucial technique for immunostaining, is often carried out on formalin‐fixed, paraffin‐embedded (FFPE) tissue sections. The role of antigen retrieval in immunostaining of ethanol‐fixed smears remains unclear. The authors evaluated the effects of 2 common antigen retrieval procedures, heat‐induced antigen retrieval and protease‐induced antigen retrieval, for immunostaining using a broad panel of antibodies.

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