The leader polypeptide of Theiler's virus is essential for neurovirulence but not for virus growth in BHK cells

A leader polypeptide of unknown function is encoded by cardioviruses, such as Theiler's murine encephalomyelitis virus. Although the deletion of this polypeptide has little effect on the growth of parental GDVII virus in baby hamster kidney (BHK) cells, the mutant virus is completely attenuated and fails to kill mice receiving intracerebral inoculations of high doses of the virus.

[1]  R. Grant,et al.  A single amino acid change determines persistence of a chimeric Theiler's virus , 1994, Journal of virology.

[2]  W. Kong,et al.  Involvement of cardiovirus leader in host cell-restricted virus expression. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[3]  R. Fujinami,et al.  Importance of amino acid 101 within capsid protein VP1 for modulation of Theiler's virus-induced disease , 1994, Journal of virology.

[4]  J. Peterson,et al.  Class II-restricted T cell responses in Theiler's murine encephalomyelitis virus-induced demyelinating disease. V. Mapping of a dominant immunopathologic VP2 T cell epitope in susceptible SJL/J mice. , 1994, Journal of immunology.

[5]  R. Roos,et al.  Chimeric cDNA studies of Theiler's murine encephalomyelitis virus neurovirulence , 1993, Journal of virology.

[6]  K. Jensen,et al.  A single base deletion in the 5' noncoding region of Theiler's virus attenuates neurovirulence , 1992, Journal of virology.

[7]  R. Roos,et al.  Pathogenesis of early and late disease in mice infected with Theiler's virus, using intratypic recombinant GDVII/DA viruses , 1992, Journal of virology.

[8]  K. Jensen,et al.  The 5' noncoding sequences from a less virulent Theiler's virus dramatically attenuate GDVII neurovirulence , 1991, Journal of virology.

[9]  W. Kong,et al.  Alternative translation initiation site in the DA strain of Theiler's murine encephalomyelitis virus , 1991, Journal of virology.

[10]  R. Fujinami,et al.  Direct evidence of a role for amino acid 101 of VP-1 in central nervous system disease in Theiler's murine encephalomyelitis virus infection , 1991, Journal of virology.

[11]  R. Roos,et al.  Strains from both Theiler's virus subgroups encode a determinant for demyelination , 1990, Journal of virology.

[12]  B. Semler,et al.  Neurovirulence determinants of genetically engineered Theiler viruses. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[13]  H. Lipton,et al.  Genomic regions of neurovirulence and attenuation in Theiler murine encephalomyelitis virus. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[14]  B. Semler,et al.  Infectious cDNA clones of the DA strain of Theiler's murine encephalomyelitis virus , 1989, Journal of virology.

[15]  B. Semler,et al.  Polyprotein processing of Theiler's murine encephalomyelitis virus , 1989, Journal of virology.

[16]  R. Fujinami,et al.  Alteration of amino acid 101 within capsid protein VP-1 changes the pathogenicity of Theiler's murine encephalomyelitis virus , 1989, The Journal of experimental medicine.

[17]  R. Lloyd,et al.  Leader protein of foot-and-mouth disease virus is required for cleavage of the p220 component of the cap-binding protein complex , 1988, Journal of virology.

[18]  J. Borkowski,et al.  Insights into Theiler's virus neurovirulence based on a genomic comparison of the neurovirulent GDVII and less virulent BeAn strains. , 1988, Virology.

[19]  S. Miller,et al.  Class II-restricted T cell responses in Theiler's murine encephalomyelitis virus (TMEV)-induced demyelinating disease. II. Survey of host immune responses and central nervous system virus titers in inbred mouse strains. , 1987, Microbial pathogenesis.

[20]  H. Lipton,et al.  Analysis of the complete nucleotide sequence of the picornavirus Theiler's murine encephalomyelitis virus indicates that it is closely related to cardioviruses , 1987, Journal of virology.

[21]  P. Chomczyński,et al.  Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. , 1987, Analytical biochemistry.

[22]  G. Parks,et al.  Encephalomyocarditis virus 3C protease: efficient cell-free expression from clones which link viral 5' noncoding sequences to the P3 region , 1986, Journal of virology.

[23]  E. Beck,et al.  A second protease of foot-and-mouth disease virus , 1986, Journal of virology.

[24]  A. Haase,et al.  Simultaneous in situ detection of viral RNA and antigens. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[25]  H. Lipton,et al.  Theiler's virus antigen detected in mouse spinal cord 2½ years after infection , 1984, Neurology.

[26]  H. Lipton,et al.  Replication of Theiler's murine encephalomyelitis viruses in BHK21 cells: an electron microscopic study. , 1980, Virology.

[27]  H. Lipton,et al.  The TO strains of Theiler's viruses cause “slow virus—like” infections in mice , 1979, Annals of neurology.

[28]  P. Tegtmeyer,et al.  Identification of simian virus 40 protein A , 1977, Journal of virology.

[29]  H. Lipton,et al.  Chronic neurologic disease in Theiler's virus infection of SJL/J mice , 1976, Journal of the Neurological Sciences.

[30]  F. Hochberg,et al.  Demyelinative myelopathy in mice induced by the DA virus , 1976, Journal of the Neurological Sciences.

[31]  H. Lipton Theiler's virus infection in mice: an unusual biphasic disease process leading to demyelination , 1975, Infection and immunity.

[32]  M. Theiler,et al.  ENCEPHALOMYELITIS OF MICE : I. CHARACTERISTICS AND PATHOGENESIS OF THE VIRUS. , 1940 .

[33]  M. Theiler,et al.  ENCEPHALOMYELITIS OF MICE , 1940, The Journal of experimental medicine.

[34]  H. Lipton Persistent Theiler's murine encephalomyelitis virus infection in mice depends on plaque size. , 1980, The Journal of general virology.